Ciliate desert-grass regeneration method via green spherical body and special culture medium thereof

A technology of green spheroids and growth medium is applied in the field of centipede grass regeneration plants through green spheroids, can solve the problem of not establishing a centipede grass genetically transformed plant regeneration system, etc., and achieves the effects of low cost, short time and simple operation.

Inactive Publication Date: 2009-05-06
INST OF BOTANY CHINESE ACAD OF SCI
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  • Abstract
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AI Technical Summary

Problems solved by technology

Although some people have used Centipede grass spores to carry out rapid tissue culture propagation to provide enough seedlings for the remediation of soil arsenic pollutio

Method used

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  • Ciliate desert-grass regeneration method via green spherical body and special culture medium thereof
  • Ciliate desert-grass regeneration method via green spherical body and special culture medium thereof
  • Ciliate desert-grass regeneration method via green spherical body and special culture medium thereof

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Embodiment 1

[0026] The plant regeneration of embodiment 1, centipede grass

[0027] One, plant regeneration I of centipede grass

[0028] 1. Spore germination and gametophyte and sporophyte culture of Centipede grass

[0029] Collect 0.1 g of mature centipede grass (Pteris vittata L) spores from the greenhouse, sterilize them with 0.3% NaClO for 10 min in a 1.5 ml centrifuge tube, then centrifuge at 13,000 g / min for 5 min, collect the bottom spores after centrifugation and rinse with sterile water 4 times, suspended in 0.5ml sterile water, and then inoculated in the following spore germination, gametophyte, sporophyte medium, cultured at 25°C, with a photoperiod of 15h light / 9h dark, and a light intensity of 2400lux.

[0030] The composition of the spore germination, gametophyte and sporophyte growth medium is as follows: 1 / 2 MS+20g / L sucrose+5g / L agar; pH value is 5.9.

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Abstract

The invention discloses a method for culturing the green spheroids of a pteris vittata and a special medium thereof. In the method, the rhizomes of ferns are used as explants and are cultured by a plant tissue medium to obtain the green spheroids; the plant tissue medium is a medium obtained by adding cane sugar with a final concentration of 18 to 22g/L, agar with a final concentration of 4 to 6g/L, GA with a final concentration of 0.3 to 0.7g/L and 6-BA with a final concentration of 0.4 to 0.6g/L into a 1/2MS-MS medium; wherein, the final concentration refers to the concentration in the plant tissue medium. Experiments show that the induction rate of the green spheroids obtained by the medium and the culturing method can achieve more than 70 percent. The differentiation rate of the green spheroids to carry out bud initiation can achieve 98 percent. Moreover, the method has the characteristics of short time, low cost and simple operation.

Description

technical field [0001] The invention relates to a method for regenerating plants of centipede grass through green spheroids and a special culture medium thereof. Background technique [0002] Arsenic contamination of soil and water has become a major global environmental problem (Meharg, 2004). Tens of millions of people in the world are currently threatened by the chronic toxic effects of arsenic-contaminated soil, groundwater, and various foods (Smith et al., 1992). The fern centipede grass (Pteris vittata L), as the first plant reported to have the function of arsenic hyperaccumulation, can be applied to the phytoremediation of arsenic-contaminated soil (Ma et al., 2001). However, so far, the mechanism of accumulation and detoxification of arsenic by Centipede grass is not clear. The important reason is that the sporophyte of Centipede grass is a plant with a large genome and slow growth, so it is difficult to detect it with genetic methods such as mutant induction. To ...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 麻密戴晓静郑永强徐文忠何振艳
Owner INST OF BOTANY CHINESE ACAD OF SCI
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