Long-acting protein solution stabilizing agent

A protein solution and stabilizer technology, applied in biological testing, material inspection products, etc., can solve the problems of Tween 20 color depth, complex components, peculiar smell, etc., and achieve the effect of obvious effect, simple composition and maintenance of effectiveness.

Inactive Publication Date: 2013-12-25
BIOLOGY INST OF HEBEI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this patent has a good stabilizing effect, the composition is relatively complex. Among them, surfactants are widely used in the field of biological sciences, but they still have some biological toxicity. For example, Tween surfactants have hemolysis, and Tween 20 Dark color and odor; although the addition of antipyrine and gentamicin sulfate can help improve the stability of the enzyme and inhibit bacterial growth, it may increase the background in the enzyme binding test and affect the test results

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Bovine Serum Albumin 0.5g

[0033] Mannose 10g

[0034] Sorbitol 10g

[0035] ProClin300 0.05% (m / v)

[0036] Phosphate buffer 100mM, pH7.4

[0037] EDTA Disodium Salt 5mmol

[0038] Add water to make up to 100ml.

[0039] Preparation:

[0040] Prepare 50ml of 200mM, pH7.4 phosphate buffer buffer; add the above-mentioned weight of mannose, sorbitol, ProClin300 and edetate disodium salt to the prepared buffer; after fully dissolving, add the above-mentioned weight Bovine serum albumin, add deionized water to make up to 100ml. Finally, adding bovine serum albumin can further improve the stability of the solution in this example.

[0041] Detection method:

[0042] Dilute 1 μL of horseradish peroxidase (protein) concentrated solution with 1000 μL of the stabilizing solution of Example 1, and use the working solution of horseradish peroxidase without stabilizer as a control group, and package normally.

[0043] The two solutions were placed in an environment of 37°...

Embodiment 2

[0048] Recombinant Human Serum Albumin 1g

[0049] Inulin 8g

[0050] Mannitol 5g

[0051] ProClin200 0.1% (m / v)

[0052] Phosphate buffer 150mM, pH7.2

[0053] EDTA disodium salt 1mmol

[0054] Add water to make up to 100ml.

[0055] Preparation:

[0056] Prepare 50ml of 300mM, pH7.2 phosphate buffer buffer; add the above-mentioned weight of inulin, mannitol, ProClin200 and EDTA disodium salt to the prepared buffer; after fully dissolving, add the above-mentioned weight Recombinant human serum albumin, add deionized water to make up to 100ml. Finally, adding recombinant human serum albumin can further improve the stability of the solution in this example.

[0057] Detection method:

[0058] Dilute 1 μL of horseradish peroxidase (protein) concentrated solution with 1000 μL of the stabilized solution of Example 2, and use the horseradish peroxidase working solution without stabilizer as a control group, and package normally.

[0059] The two solutions were placed in an...

Embodiment 3~6

[0064]

[0065] Detection method:

[0066] Dilute 1 μL of horseradish peroxidase (protein) concentrated solution with 1000 μL of the stabilized solution of Examples 3-6, and use the horseradish peroxidase working solution without stabilizer as a control group, and package normally.

[0067] The resulting solution was placed in a 37°C environment for 20 days, and the 0-day horseradish peroxidase solution was used as a control, and the enzyme-linked immunoassay (using an anti-thyroid hormone antibody detection kit) was used to detect and calculate the enzyme levels of the two solutions. residual activity.

[0068] As can be seen from the data in Table 3, the solution that does not add stabilizer loses activity very soon, and the solution that adds stabilizer as time goes on, its activity remains unchanged substantially, shows the stabilizer effect of the present invention very good.

[0069] Table 3. The results of the detection of the stability of the microtiter plate of...

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Abstract

The invention relates to a stabilizing agent for protein solutions, in particular to a long-acting protein solution stabilizing agent. The agent comprises 0.1-5g protein, 0.1-10g sugar, 0.1-10g organic matter, 0.01-0.1g preservative, and 10-200mmol buffer solution salt per 100ml water, and a pH (Potential of Hydrogen) value is 7.2-7.4. The agent has simple constituents, protects the stability of the protein, prevents the protein from degeneration, does not interfere with target reaction, and has general applicability to the most protein solutions. The agent has obvious effects, and can keep the effectiveness of the protein solutions for a long time, and the protein solutions are to be used for the fields of reagents for clinical diagnosis, life science research and the like.

Description

technical field [0001] The present invention relates to a stabilizer for protein solutions. Background technique [0002] In clinical testing and life science research, protein detection reagents not only have a certain sensitivity and specificity, but also their repeatability and stability are also very important. Good stability is an important symbol of its data reliability. However, some enzymes and protein markers have very poor stability and are easily degraded or denatured under normal conditions, resulting in the overall failure of the detection reagent. For example, in chemiluminescent immunoassay (CLIA), enzyme conjugates are easily inactivated after dilution, and the reduction or loss of enzyme activity will directly affect the accuracy of the detection results. Therefore, how to maintain the stability of protein, especially enzyme activity, is a key technology in protein detection. [0003] In recent years, people have discovered some substances that help to im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
Inventor 程华闫静辉李春生张小兵陈英珠
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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