Recovery liquid and method for cryopreserved epidermal stem cells

Abstract

The invention discloses a recovery liquid and a recovery method for cryopreserved epidermal stem cells. According to the key point of the technical scheme, the recovery liquid is prepared from the following components in parts by weight: 20-25 parts of carbohydrates, 0.06-0.08 part of epidermal growth factors, 0.06-0.08 part of alkaline fibroblast growth factors, 0.01-0.02 part of polylysine, 0.01-0.02 part of glial cell line derived neurotrophic factors, 0.1-0.25 part of trypsin and 10-15 parts of fetal calf serum. According to the technical scheme, good recovery effect of the epidermal stem cells is achieved, and the growth environment of the epidermal stem cells is relatively proper.

Description

technical field [0001] The invention relates to the field of cells, in particular to a recovery method for cryopreserved epidermal stem cells. Background technique [0002] Stem cells with the ability to continuously proliferate and differentiate at the base of the epidermis are called epidermal stem cells. Epidermal stem cells are multipotent stem cells in skin tissue. They are adult tissue stem cells that can proliferate and differentiate into various cell components in the epidermis to maintain the normal epidermal structure of the skin. [0003] Epidermal stem cells, as tissue-specific stem cells, have high proliferative ability and the ability to differentiate into full-thickness epidermis, and are important seed cells for tissue-engineered skin. [0004] Cryopreservation is one of the core technologies in the research and application of stem cells. A safe and efficient cryopreservation method for epidermal stem cells is a necessary condition for the widespread use of ...

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Problems solved by technology

[0005] Ma Yingzhi. Using human epidermal stem cells and human dermal fibroblasts to construct tissue engineering conformable skin in vitro[D]. Jilin University, 2009. In the paper, human epidermal stem cells were used as seed cells, and rat tail collagen was used to prepare dermal scaffold materials. In vitro construction of tissue engineered composite skin, page 28 of which introduces the cryopreservation and recovery of epidermal stem cells. In practical applications, the effect of freezing and recovery of epidermal stem cells is not ideal. Epidermal stem cells cultured in vitro often rupture and are relatively fragile. The survival rate is relatively low

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