Bacillus cereus, and preparation and application of bacillus cereus

A technology for Bacillus cereus and microbial preparations is applied in the field of preparation of Bacillus cereus and the production of microbial preparations by using the Bacillus cereus, and can solve problems such as restricting the healthy and sustainable development of the industry, lagging disease prevention technology, economic losses and the like, To achieve the effect of fast reproduction, food safety, and reduction of drug residues

Inactive Publication Date: 2012-07-11
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, due to the high-density breeding and non-standard operation of sea cucumbers and the relative lag of disease prevention and control technology, disease problems have become increasingly prominent. Healthy and sustainable development

Method used

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  • Bacillus cereus, and preparation and application of bacillus cereus
  • Bacillus cereus, and preparation and application of bacillus cereus
  • Bacillus cereus, and preparation and application of bacillus cereus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Screening and isolation process of Aj080319IA-16 strain:

[0042] The Aj080319IA-16 strain was isolated from the intestines of wild sea cucumbers by the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences. In 2008, the Yellow Sea Fisheries Research Institute of the Chinese Academy of Fishery Sciences captured wild sea cucumbers from the waters of Yantai. Under sterile conditions, the sea cucumbers were dissected, and the intestines were taken, and the contents of the intestines were squeezed out, and sterilized with 15‰ Wash the intestinal tract with normal saline three times, homogenate the washed intestinal tract, spread it on the TSB medium and 2216E seawater agar medium after equal proportion dilution, select the appropriate dilution plate to separate the bacteria and pass multiple strokes Bacteria were purified by line culture, and the morphological structure and quantity of bacteria were recorded and photographed; the common pathogenic...

Embodiment 2

[0047] 16SrDNA sequence determination:

[0048] Such as figure 1 Shown is the results of the phylogenetic tree obtained by using the PHYLIP software according to the 16S rDNA sequence described in the embodiment of the present invention.

[0049] The general primers used to amplify bacterial 16S rDNA were 27F: 5’-AGAGTTTGATC(C / A)TGGCTCAG-3’ and the reverse primer 1492R: 5’-TACGG(C / T)TACCTTGTTACGACTT-3’. The 16S rDNA was amplified using the genomic DNA of the Aj080319IA-16 strain as a template, and then transformed and sequenced. See the sequence table for the obtained sequence. The obtained 16S rDNA sequence of the Aj080319IA-16 strain was compared in Genbank for homology, and Clustalw software was used for multiple sequence matching alignment, and the phylogenetic inference software package PHYLIP4.0 was used for statistical analysis and clustering analysis, and the neighbor linking method was used Obtain a phylogenetic tree ( figure 1 ), the phylogenetic tree was evaluate...

Embodiment 3

[0051] Physiological and biochemical characteristics

[0052] The physiological and biochemical characteristics of the strains were analyzed with the APIID32E identification reagent strip of the French Mérieux company. For uncertain reactions, the strain identification tubes of Beijing Land Bridge Technology Co., Ltd. were used for determination. The results are shown in Table 1. According to the characteristics of "Bergey's Manual of Determinative Bacteriology" (9th edition) and Gordon's "Bacillus" genus, the strain was classified and identified. The -16 strain belongs to a species of Bacillus cereus.

[0053] Table 1: Physiological and biochemical reaction results of Aj080319IA-16 strain APIID32E

[0054]

[0055]

[0056] Note: +: Indicates positive reaction; -: Indicates negative reaction; ND: Indicates indeterminate reaction.

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Abstract

The invention discloses a bacillus cereus, and preparation and application of the bacillus cereus. A bacillus cereus strain Aj080319IA-16 is separated from an intestinal tract of a wild stichopus japonicus selenka and collected in China Center for Type Culture Collection (CCTCC) along with a collection number of CCTCC M 2010315. The screened bacillus cereus has remarkable antagonistic action on pathogenic bacteria of a sea cucumber, and has the effects of increasing the growth speed, immunity and intestinal digestive enzyme activity of the stichopus japonicus selenka. The screened bacillus cereus is safe and non-toxic for the sea cucumber, fast in breeding speed, easy to culture and strong in adaptability on environment, and is capable of being bred quickly to form predominant bacteria. The researched and developed microbiological preparation is simple and convenient to use and operate and only needs to be added into a feed for feeding, so that the preparation is suitable for young stichopus japonicus selenka breeding and disease control in a variety of stichopus japonicus selenka breeding processes. By means of the preparation, the use of antibiotics can be reduced or replaced, and an important role is played in reducing chemical residues, improving the product quality of the sea cucumber and guaranteeing the food safety.

Description

technical field [0001] The invention relates to the technical field of aquatic application microorganisms, in particular to a bacillus cereus and a preparation method and application of using the bacillus cereus to produce microbial preparations. Background technique [0002] Intestinal probiotics are a live bacterial preparation that mostly originates from the digestive tract and acts on the digestive tract. It can effectively supplement the beneficial microorganisms in the digestive tract of animals, inhibit the growth of pathogenic bacteria, improve the balance of digestive tract flora, and improve the immune function of aquatic animals. or growth rate. [0003] Sea cucumber (Apostichopus japonicusLiao, also known as imitation sea cucumber), belongs to invertebrates, Echinodermata, sea cucumber class, and sea cucumber genus. Because of its high nutritional value, the scale of cultivation continues to expand, and it has developed into one of the largest marine aquaculture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/085
Inventor 荣小军王岚王印庚张正廖梅杰李彬陈贵平陈霞薛太山
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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