Long-chain non-coding RNA sequence for early diagnosis of human lung adenocarcinoma, preparation and application

Abstract

The invention discloses an overall-length cDNA sequence for long-chain non-coding RNA, preparation and application. The cDNA sequence of the long-chain non-coding RNA is as shown in SEQ ID NO. 1, the overall-length gene sequence of accurate linc00857 is cloned for the first time, a foundation is laid for research of functions of linc00857 in a generation and development process of adenocarcinoma of lung, high expression of the long-chain non-coding RNA in the adenocarcinoma of lung is obtained by analysis, expression in normal lung tissues is relatively low, expression of the long-chain non-coding RNA is remarkably related to progression of the adenocarcinoma of lung, therefore, the invention further discloses application of the long-chain non-coding RNA in preparation of products for diagnosing or treating the adenocarcinoma of lung, for example, a short hairpin fragment of an RNAi target site of the long-chain non-coding RNA and expression of recombinant plasmids of transcribed short hairpin RNA in cells of the adenocarcinoma of lung can obviously degrade cell endogenous long-chain non-coding RNA and inhibit malignant growth of the cells of the adenocarcinoma of lung.

Description

technical field [0001] The invention belongs to the field of tumor molecular biology, and specifically relates to a precise cDNA sequence of a lung adenocarcinoma-specific long-chain non-coding RNA, a preparation method of the sequence, a short hairpin fragment for RNA interference of the gene, and an application thereof. Background technique [0002] Lung cancer is one of the cancers with the highest morbidity and mortality in the world, and it shows an increasing trend year by year. In my country, the morbidity and mortality of lung cancer also rank first among all cancers. Small cell lung cancer (Small Cell Lung Cancer, SCLC) accounts for about 15% of lung cancer patients, and non-small cell lung cancer (Non-Small Cell Lung Cancer, NSCLC) accounts for about 80%. Lung adenocarcinoma is a type of non-small cell lung cancer. Most adenocarcinomas originate from the smaller bronchi and are peripheral lung cancers, which tend to occur in women and non-smokers. The five-year su...

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Problems solved by technology

However, the technical solutions in the above documents still have the following defects: 1. The accurate full-length sequence of linc00857 is of great significance for studying the function of linc00857 in the occurrence and development of lung adenocarcinoma, but the above-mentioned documents fail to provide the accurate full-length sequence of linc00857 sequence, it is difficult to meet the requirements of functional research in the occurrence and development of lung adenocarcinoma; 2. The molecular marker linc00857 provided in the above literature has few RNA interference targets, which cannot provide a more effective treatment plan and affects its gene therapy effect, as mentioned above Although the literature provides siRNA sequences and shRNA plasmids for interfering with linc00857 in lung adenocarcinoma cells, the interference efficiency of shRNA plasmids is low, less than 50%. Although siRNA can achieve higher interference efficiency, it is the same as shRNA Compared with interference, siRNA can only perform transient interference on cells, and is highly unstable and prone to off-target effects. This off-target effect seriously affects the application of siRNA in clinical gene therapy of tumors.

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