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Methylation molecular markers for diagnosing lung adenocarcinoma and application of methylation molecular markers for diagnosing lung adenocarcinoma

A molecular marker and methylation technology, applied in the field of biomedical detection, can solve problems such as the survival rate of less than 20%, unsatisfactory survival of lung adenocarcinoma patients, and no report on the EID3 gene, achieving high detection accuracy, Accurately detect the effect of the method

Pending Publication Date: 2020-05-22
HUADONG HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the pathological subtypes of lung adenocarcinoma are diverse, which brings challenges to the diagnosis of lung adenocarcinoma
Low-dose lung CT is currently the main method for screening lung cancer, which enables a large number of patients with lung adenocarcinoma to be detected in the early stage of tumor occurrence. The prognosis of patients with lung adenocarcinoma has improved to some extent, but the overall survival period of patients with lung adenocarcinoma is still not ideal, and the 5-year survival rate is less than 20%.
[0004] At present, the effect of EID3 gene and its methylation in lung adenocarcinoma has not been reported
And there is no complete kit for detecting EID3 gene expression and methylation expression in tissues

Method used

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  • Methylation molecular markers for diagnosing lung adenocarcinoma and application of methylation molecular markers for diagnosing lung adenocarcinoma
  • Methylation molecular markers for diagnosing lung adenocarcinoma and application of methylation molecular markers for diagnosing lung adenocarcinoma
  • Methylation molecular markers for diagnosing lung adenocarcinoma and application of methylation molecular markers for diagnosing lung adenocarcinoma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1 TCGA methylation database research

[0027] 1. Data download:

[0028] The TCGA lung adenocarcinoma methylation database was downloaded from the official website of the TCGA database at https: / / tcga-data.nci.nih.gov / tcga / , and the search keywords were lung adenocarcinoma and DNA methylation. The data download date is 2019-03-27. All data were normalized and the total sample size of the DNA methylation cohort was 616 (53 normal, 563 lung adenocarcinoma).

[0029] 2. Data processing:

[0030] (1) All data were normalized before further analysis.

[0031] (2) Chi-square test was used to analyze the difference of gene methylation in lung adenocarcinoma and adjacent lung tissue.

[0032] (3) Differentially methylated genes were screened using p=0.05 and |logFc|=0.5 as criteria.

[0033] (4) The TCGA data download software is Perl, and the software used for data processing and analysis is R with version number 3.3.1.

[0034] 3. Data analysis results:

[0035...

Embodiment 2

[0035] In the present invention, 616 sample information were included in the TCGA gene methylation cohort, including 563 lung adenocarcinoma cases and 53 control cases. We used p-value 0.5 for screening, and found that among the 20204 genes with methylation data, a total of 2084 genes were differentially methylated between lung adenocarcinoma tissue and paracancerous tissue, of which There were 369 hypermethylated tumor tissues and 1715 relatively hypomethylated tumor tissues compared with paracancerous tissues. We show a heat map of the top 100 differentially methylated genes ( figure 1 ). Example 2 Quantitative study on tissue EID3 methylation level

[0036] 1. Research object:

[0037] The 30 cases of lung adenocarcinoma and adjacent normal lung tissue samples we used were from patients who were diagnosed with lung adenocarcinoma by the Department of Thoracic Surgery of East China Hospital and underwent surgical resection. Immediately after resection, the obtained tissu...

Embodiment 3

[0097] Embodiment 3: clinical validation

[0098] According to the above method, we detected the methylation level of EID3 gene in 30 samples (including lung adenocarcinoma tissue and adjacent normal tissue) in East China Hospital, and further analyzed the methylation level of each methylation site included in the tumor and adjacent normal tissue. difference in methylation. The inventors found that, in East China Hospital samples, EID3 showed significant hypermethylation in the lung adenocarcinoma group relative to the paracancerous tissues ( image 3 ). And among the detected methylation sites, except for 194, 204, and 208 sites, the methylation levels of other sites are relatively low. The methylation of all sites in the T group was significantly higher than that in the N group ( Figure 4 ). Through cluster analysis, the present inventors found that in lung adenocarcinoma and adjacent tissues, there is a significant difference in the level of RHOH methylation, and it ca...

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Abstract

The invention provides methylation molecular markers for diagnosing lung adenocarcinoma and application of the methylation molecular markers for diagnosing lung adenocarcinoma. The methylation molecular markers are methylated EID3 genes, and by measuring expression of EID3 genes and the methylation levels in samples, lung adenocarcinoma is diagnosed. According to the methylation molecular markersfor diagnosing lung adenocarcinoma and application of the methylation molecular markers for diagnosing lung adenocarcinoma, the fact that the methylated EID3 genes can be used as diagnosis markers oflung adenocarcinoma is proven for the first time due to the fact that the EID3 are significantly hypermethylated in lung adenocarcinoma tissue relative to the EID3 in paracancerous normal lung tissue;and specific high expression of the methylated EID3 genes in the lung adenocarcinoma tissue provides a novel path for diagnosis of lung adenocarcinoma. A kit used for detecting the methylated EID3 genes is provided. The kit used for detecting the methylated EID3 genes is high in detection accuracy, so that a faster and more accurate detection method is provided for diagnosis of lung adenocarcinoma.

Description

technical field [0001] The invention belongs to the technical field of biomedical detection, and relates to the application of methylated EID3 as a molecular marker for diagnosing lung adenocarcinoma and preparing reagents or kits for diagnosing lung adenocarcinoma. Background technique [0002] The latest clinical data show that lung cancer is one of the malignant tumors with the highest morbidity and mortality in the world. Lung adenocarcinoma is the most common pathological type. A study on non-smoking lung adenocarcinoma in my country showed that compared with other pathological types of lung adenocarcinoma, lung adenocarcinoma has more gene mutations. In addition, the pathological subtypes of lung adenocarcinoma are diverse, which brings challenges to the diagnosis of lung adenocarcinoma. Low-dose lung CT is currently the main method for screening lung cancer, which enables a large number of patients with lung adenocarcinoma to be detected in the early stage of tumor ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/154C12Q2600/158
Inventor 匡牧宇张辉标孙艺华周子琅肖健曹佳实
Owner HUADONG HOSPITAL
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