Group of LncRNA biological markers for diagnosing human lung squamous carcinoma

A biomarker, lung squamous cell carcinoma technology, applied in the biological field, can solve problems such as the lack of a good evaluation of the potential of lncRNA

Active Publication Date: 2016-01-06
SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the expression of lncRNA related to lung squamous cell carcinoma is still in the preliminary stage, and the potential of lncRNA as a tumor marker of lung squamous cell carcinoma has not yet been well evaluated.

Method used

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  • Group of LncRNA biological markers for diagnosing human lung squamous carcinoma
  • Group of LncRNA biological markers for diagnosing human lung squamous carcinoma
  • Group of LncRNA biological markers for diagnosing human lung squamous carcinoma

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1 Clinical sample collection:

[0043] 63 pairs of fresh frozen tissue specimens of early lung squamous cell carcinoma and their paired normal lung tissues were collected from Zhongshan Hospital Affiliated to Fudan University and the Institute of Cells, Chinese Academy of Sciences during 2013-2014. All patients gave informed consent to participate in the scientific research.

[0044] 2LncRNA quantitative PCR experiment:

[0045] 2.1 RNA extraction and quantitative PCR

[0046] Total RNA was extracted from frozen tissues using Trizol, and RNA was quantified using a microvolume spectrophotometer.

[0047] 2.2 Internal reference genes and primers

[0048] The internal reference gene selects β-actin, β-actin and a set of primer sequences of LncRNA biomarkers provided by the present invention as shown in Table 1:

[0049] Table 1 Primer sequences used in quantitative PCR

[0050]

[0051]

[0052] 2.3 Reverse transcription reaction

[0053] Use the SuperScriptⅢFi...

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Abstract

The invention relates to the field of biotechnologies, and in particular relates to a group of LncRNA biological markers for diagnosing human lung squamous carcinoma, a related kit and applications thereof. The group of LncRNA biological markers for diagnosing the human lung squamous carcinoma, provided by the invention, comprises ENST00000453324, ENST00000441841, uc011cly.2, NR_028500 and NR_046326. The LncRNA biological markers for diagnosing the human lung squamous carcinoma can be used for distinguishing human early lung squamous carcinoma, the AUC value of the LncRNA biological markers for distinguishing the early lung squamous carcinoma and pairing normal lung tissues can reach 0.932, and the sensitivity and the specificity can be 92% and 87% respectively.

Description

technical field [0001] The present invention relates to the field of biotechnology, in particular to a group of LncRNA biomarkers for diagnosis of human lung squamous cell carcinoma, related kits and their uses. Background technique [0002] Non-small cell lung cancer is one of the leading causes of cancer-related death worldwide. It mainly includes four histological subtypes: adenocarcinoma, squamous cell carcinoma, large cell carcinoma, and others (neuroendocrine carcinoma, benign tumor, etc.). Squamous cell carcinoma accounts for about 30% of all non-small cell lung cancers, and its lethality is second only to adenocarcinoma. Squamous cell carcinoma of the lung is more common in elderly men and is closely related to smoking. Since the five-year survival rate of non-small cell lung cancer is less than 15%, early diagnosis can effectively reduce its mortality. [0003] Low-dose spiral CT has high sensitivity in the screening of early lung cancer, especially in pulmonary n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68C40B40/06
Inventor 毛红菊程祖乐冒海蕾白亚楠张宏莲金庆辉赵建龙
Owner SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI
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