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New molecular markers for detection of squamous cell carcinomas and adenocarcinomas and high-grade precursor lesions thereof

A technology for squamous cell carcinoma, grade carcinoma, applied in the field of diagnostics, can solve the problem of lack of sensitivity in malignant diseases

Inactive Publication Date: 2010-09-08
VER VOOR CHRISTELIJK HOGER ONDERWIJS WETENSCHAPPELIJK ONDERZOEK & PATIENTENZORG
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  • Application Information

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Problems solved by technology

However, the heterogeneity of events capable of driving malignant transformation of cells with perturbed p53 and Rb pathways suggests that a single marker lacks sufficient sensitivity for malignancy

Method used

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  • New molecular markers for detection of squamous cell carcinomas and adenocarcinomas and high-grade precursor lesions thereof
  • New molecular markers for detection of squamous cell carcinomas and adenocarcinomas and high-grade precursor lesions thereof
  • New molecular markers for detection of squamous cell carcinomas and adenocarcinomas and high-grade precursor lesions thereof

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Experimental program
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Embodiment

[0081] 1. Differentially expressed genes in tissues and cell lines

[0082] To confirm the differential expression of the genes listed in Tables 1 and 2, we measured the mRNA expression of the following genes by real-time RT-PCR analysis: ITGAV, SYCP2, DTX3L, SEMP1, DEK, ATP2C1, SLC25A36, and PIK3R4. For this purpose, samples from 22 cervical squamous cell carcinomas, 8 cervical adenocarcinomas, 15 high-grade cervical epithelial neoplasia (CIN) lesions, and 24 normal epithelial controls (n = 13 for normal RNA was extracted from neck n=11). ITGAV, SYCP2, DTX3L, SEMP1, DEK, ATP2C1, PIK3R4, and SLC25A36 showed significant overexpression in squamous cell carcinomas compared with normal ectocervix (see e.g. figure 1 ). Additionally, DTX3L showed significant overexpression in adenocarcinoma compared with normal endocervix ( figure 1 ). Analysis of RNA isolated from 15 high-grade CIN lesions enriched in dysplastic cells by laser capture microdissection confirmed increased express...

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Abstract

The present invention provides for new markers for the detection of HPV-induced or lung squamous cell carcinoma and / or adenocarcinoma and high-grade precursor lesions thereof, comprising the genes of the present invention. Detection can take place by detection over expression or down regulation of said genes either by nucleic acid assay or by assays for the proteins produced by said genes.

Description

technical field [0001] The present invention relates to the field of diagnostics, especially the diagnosis of cancer, more particularly, the present invention relates to the diagnosis of squamous cell carcinoma and adenocarcinoma and their high-grade precursor lesions. Background technique [0002] Squamous cell and adenocarcinoma development is characterized by a series of premalignant lesions graded as mild dysplasia, moderate dysplasia, and severe dysplasia / carcinoma in situ in situ). Squamous cell and adenocarcinomas can grow from the mucosal lining of several organs, such as the cervix, vagina, anus, head and neck, and lungs. [0003] During the past decade, it has been well established that squamous cell carcinoma and even a subset of adenocarcinomas can be initiated by infection with high-risk human papillomavirus (hrHPV). Epidemiological and functional studies involving cervical cancer have made this causality more apparent (zurHausen, Nat Rev Cancer 2002; 2:342-35...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6886A61P35/00C12Q2600/158
Inventor 克里斯托弗鲁斯·琼内斯·拉姆贝图斯·玛丽亚·迈耶彼得鲁斯·约瑟夫斯·费丁安杜斯·斯尼杰德尔斯伦斯克·丹尼拉·玛丽亚·斯泰恩贝尔根
Owner VER VOOR CHRISTELIJK HOGER ONDERWIJS WETENSCHAPPELIJK ONDERZOEK & PATIENTENZORG
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