17 results about "Nucleic acid assay" patented technology

The present invention relates to CRISPR / Cas based biosensing materials, assays, and methods. Specifically, the present technology relates to CRISPR / Cas-based ultra-sensitive detection methods for nucleic acid assays using specific molecular constructs, including constructs referred to as nucleic acid mediators, comprising single-stranded and double-stranded nucleic acid sequences of a cyclic conformation, and palindromic oligonucleotides. The materials and methods according to the invention can also be used to enhance the sensitivity of existing bioassays.

Disclosed herein are methods and systems of determining a cancer status or an organ health status of a subject, the method comprising: obtaining a biological sample obtained or derived from the subject; enriching a population of cells in the biological sample, wherein the population of cells comprises stem cells and / or progenitor cells; extracting nucleic acids from the enriched population of cells; assaying the extracted nucleic acids to generate at least one of a transcriptomic profile of the subject, a genomic profile of the subject, and / or an exomic profile of the subject; computer processing the at least one of the transcriptomic profile of the subject, the genomic profile of the subject, and / or the exomic profile of the subject; and determining, based at least in part on the computer processing, the cancer status or the organ health status of the subject, wherein the cancer status or the organ health status comprises a presence or an absence of an organ and / or a tissue impacted by the cancer or impacted by a disease, disorder, or condition.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The invention discloses a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application of the functional molecular marker. The invention belongs to the field of nucleic acid determination and detection, and particularly relates to a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application of the functional molecular marker. The invention provides an application of a Pm61 gene InDel molecular marker or a substance for detecting the molecular marker, wherein the InDel molecular marker is a double-stranded DNA (deoxyribonucleic acid) molecule of 19-123 sites of which the nucleotide sequence of one chain is SEQ ID No: 1; the InDel molecular marker can be applied to any one of the following applications: 1) application in identification or auxiliary identification of wheat powdery mildew resistance; and 2) application in wheat breeding.

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and / or quenching moiety.

The present disclosure provides a protein having luciferase activity comprising a secondary structural arrangement H1-L1-H2-L2-B1-L3-B2-L4-H3-L5-B3-L6-L4-L7-L5-L8-B6 wherein (i) "H" is a helical domain, "L" is a loop domain, and "B" is a beta chain domain wherein the length of the B5 domain is at least 11, 12, 13, or 14 amino acids, and wherein "L" is a cyclic domain, and "B" is a beta chain domain. And residue 11 of the B5 domain is F, Y or L; and the length of the B4 domain is at least 12 amino acids, and the residue 10 of the B4 domain is F, Y, L, I, K or M; and / or residue 12 of the B4 domain is F, L, R, D, M, Q or V; (ii) the H1 domain is at least 18 or 19 amino acids in length; the residue 9 of the H1 domain is T, S, H, R, C, L, D, V, A, Q, G, E, K, I, N or M; (iii) the length of the B3 domain is at least 6, 7, 8, 9 or 10 amino acids, and the residue 1 of the B3 domain is W or H; (iv) the length of the B4 domain is at least 12 amino acids, and the residue 10 of the B4 domain is F, Y, L, I, K or M; or (v) the length of the B4 domain is at least 12 amino acids, and the residue 12 of the B4 domain is L, R, D, M, Q, or V. Additional proteins disclosed herein are illustrated in the claims. Also provided herein are luciferase substrates, assay buffers, and kits comprising one or more of a protein having luciferase activity or a nucleic acid encoding the protein, an assay buffer, and a luciferase substrate.

The present invention generally relates to systems and methods for imaging or determining nucleic acids, for instance, within cells. In some embodiments, the transcriptome of a cell may be determined. Certain embodiments are directed to determining nucleic acids, such as mRNA, within cells at relatively high resolutions. In some embodiments, a plurality of nucleic acid probes may be applied to a sample, and their binding within the sample determined, e.g., using fluorescence, to determine locations of the nucleic acid probes within the sample. In some embodiments, codewords may be based on the binding of the plurality of nucleic acid probes, and in some cases, the codewords may define an error-correcting code to reduce or prevent misidentification of the nucleic acids. In certain cases, a relatively large number of different targets may be identified using a relatively small number of labels, e.g., by using various combinatorial approaches.

The present disclosure provides methods, systems, and chips for nucleic acid determination. Methods as described herein may comprise providing a surface comprising a pixel with a nucleic acid coupled thereto. Methods as described herein may further comprising directing a light to the pixel. Methods as described herein may further comprise detecting a Raman optical signal from the surface. The Raman optical signal may be generated upon the light interacting with the nucleotide. Methods as described herein may further comprise processing the Raman optical signal to determine a nucleotide of the nucleic acid.

The application discloses a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application thereof. The application belongs to the field of nucleic acid determination and detection, and particularly relates to a functional molecular marker of a wheat powdery mildew resistance related gene Pm61 and application thereof. The application provides application of a Pm61 gene InDel molecular marker or a substance for detecting the molecular marker, wherein the InDel molecular marker is a double-stranded DNA molecule with a nucleotide sequence of a strand being 19-123 of SEQ ID No:1; the InDel molecular marker can be applied in any one of the following: 1) application in identification or auxiliary identification of wheat powdery mildew resistance; 2) application in wheat breeding.

Provided herein are compositions and methods for isolating cell-free nucleic acid, e.g., cell-free DNA, from a sample. In particular embodiments, provided herein are compositions and methods using anti-dsDNA antibodies for isolating cell-free DNA from a sample, and for providing a sample of isolated cell-free DNA, e.g., for a nucleic acid assay. In particular embodiments, the technology relates to providing cell-free DNA from a maternal sample that is enriched for fetal cell-free fetal DNA.

Provided herein are methods of sequencing comprising click chemistry bioconjugation. In some embodiments, target polynucleotide sequences on the same or different molecules are contacted with and hybridize to probes comprising click functional groups. Probes (e.g., reading probes) hybridizing to adaptor sequences adjacent to different sequences of interest (e.g., barcodes to be sequenced) can be hybridized simultaneously in large pools. In some embodiments, the provided methods achieve multiplexing without requiring separate hybridization of probes (e.g., reading probes) for each sequencing-by-ligation cycle, thereby reducing total hybridization time which is typically a most time-consuming step in in situ technologies. In some aspects, the hybridized probes (e.g., reading probes) are clicked onto detectable probes to analyze a sequence of a target polynucleotide in a sequencing-by-ligation fashion.

Methods, systems, and articles of manufacture are provided for multiplex assays of target nucleic acids using thermal barcodes. In some cases, the thermal barcodes may be used with one or more signaling entities for improving multiplex assays. At least some of the methods described herein can be performed at relatively high throughput without the need for expensive and slow nucleic acid sequencing. In some cases, these methods may be implemented using a system or article comprising a plurality of droplets containing a target nucleic acid.

A transport medium is disclosed that can be utilized for both sample collection and molecular diagnostic applications. The transport medium can be utilized with multiple types of biological samples and maintains the stability of nucleic acid present in the biological samples so that one or more nucleic acid assay target(s) present in the biological sample is not substantially degraded during storage and shipping. Also disclosed are kits containing the transport medium, mixtures that include a biological sample disposed in the transport medium, and methods of producing and using the medium.

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.

The invention relates to the technical field of nucleic acid kits, and discloses a portable palm nucleic acid kit which comprises a bottom box, a circuit board arranged in the bottom box, a clamping groove formed in the center of the surface of the upper end of the bottom box, a micro-fluidic chip clamped in the clamping groove, a sample box arranged on the surface of the upper end of the micro-fluidic chip, and a detection card arranged on one side of the sample box. And a positioning frame is arranged on the upper end surface of the bottom box and outside the clamping groove. According to the portable palm nucleic acid kit, the micro-fluidic chip and the circuit board are arranged to control fluid movement in the micro-fluidic channel, so that a sample automatically flows into a reaction area of the reagent card, manual operation errors are reduced, the detection precision is improved, the positioning frame is arranged, the sample box and the detection card can be effectively limited, and after detection is completed, the detection accuracy is improved. The sample box and the detection card are convenient to disassemble.

Disclosed are compositions and methods for performing nucleic-acid-based assays to determine the presence or absence of a target nucleic acid in a biological sample. The disclosed methods include the use of one or more oligomers that specifically hybridize to a nucleolin nucleic acid to assess the cellularity of the biological sample. Also disclosed are related composition and methods for assessing the cellularity of a biological sample for use in a molecular diagnostic assay.

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of cleavable probes that comprise a ribonucleotide position that is susceptible to endoribonuclease (e.g., RNase H) cleavage in the presence of target nucleic acid molecules. Probes of the embodiments may also comprise non-natural nucleotide linked to a reporter and / or quenching moiety.