Probes for improved melt discrimination and multiplexing in nucleic acid assays

a nucleic acid assay and detection method technology, applied in the field of molecular biology, can solve the problems of limited multiplexing capability of current real-time pcr, difficulty in quantifying the starting template, and unparallel amplification and precision capability, and achieve the effect of increasing the multiplexing capability of real-time nucleic acid amplification
US20150044680A1Active Publication Date: 2015-02-12LUMINEX
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
LUMINEX
Publication Date
2015-02-12

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Abstract

Methods and compositions for the detection and quantification of nucleic acids are provided. In certain embodiments, methods involve the use of primers or probes that comprise a non-natural nucleotide linked to a reporter. Target nucleic acids are detected by the polymerization of a complementary probe or primer that incorporated a cognate non-natural nucleotide linked to a quencher.
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Description

[0001] This application claims benefit of priority to U.S. Provisional Application Ser. No. 61 / 864,128, filed Aug. 9, 2013, the entire contents of which are hereby incorporated by reference.INCORPORATION OF SEQUENCE LISTING

[0002] The sequence listing that is contained in the file named “LUMNP0119US_ST25.txt”, which is 5 KB (as measured in Microsoft Windows®) and was created on Jul. 31, 2014, is filed herewith by electronic submission and is incorporated by reference herein.BACKGROUND OF THE INVENTION

[0003] 1. Field of the Invention

[0004] The present invention relates generally to the field of molecular biology. More particularly, it concerns the detection of nucleic acids.

[0005] 2. Description of Related Art

[0006] Polymerase chain reaction (PCR) is a molecular biology technique for enzymatically replicating DNA without using a living organism. PCR is commonly used in medical and biological research labs for a variety of tasks, such as the detection of hereditary diseases, the identificati...

Claims

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