Detection of nucleic acids

A target nucleic acid and detector technology, which is applied in the determination/inspection of microorganisms, measurement devices, preparation of samples for testing, etc., can solve problems such as inability to detect target nucleic acids
CN1434874AInactive Publication Date: 2003-08-06ORTHO-CLINICAL DIAGNOSTICS
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ORTHO-CLINICAL DIAGNOSTICS
Publication Date
2003-08-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

Homogeneous and multiplex assays for target nucleic acids hybridized to oligonucleotide probes immobilized on particulate and non-particulate substrates are described. Target nucleic acids are determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle, or by spatial resolution of oligonucleotides immobilized at specific loci on a substrate. Target-correlated fluorescence signal, originating from a target nucleic acid hybridized to the substrate-immobilized oligonucleotide is determined as a measure of the presence or amount of the target nucleic acid.
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Description

field of invention

[0001] The present invention relates to the detection of nucleic acids. More precisely, the present invention relates to the use of support-immobilized oligonucleotides and providing detectable signals in association with said support or support-immobilized oligonucleotides and in association with target nucleic acids hybridized thereto. Signaling methods to detect target nucleic acids. Background of the invention

[0002] Detection of nucleic acids is widely used to determine the presence and copy number of specific genes, known sequences, and to identify and quantify viruses, prokaryotic and eukaryotic pathogens in clinical and environmental samples. An important characteristic of nucleic acids is their ability to form sequence-specific hydrogen bonds with nucleic acids having complementary nucleotide sequences. This ability of nucleic acids to hybridize to their complementary strands has been used to advantage in techniques known ...

Claims

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