Application of novel molecular marker to preparation of kit for diagnosis and prognosis of renal clear cell carcinoma

A technique for renal clear cell carcinoma and molecular markers, applied in the field of biological detection, can solve problems such as uncertain prognosis and variable behavior, and achieve high sensitivity, good specificity, high clinical use and promotion value

Active Publication Date: 2018-09-21
ZHEJIANG UNIV
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Problems solved by technology

However, the histological behavior of clear cell renal cell carcinoma is variable, the prognosis is uncertain, and nearly 30% of patients have tumor recurrence after surgery

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  • Application of novel molecular marker to preparation of kit for diagnosis and prognosis of renal clear cell carcinoma
  • Application of novel molecular marker to preparation of kit for diagnosis and prognosis of renal clear cell carcinoma
  • Application of novel molecular marker to preparation of kit for diagnosis and prognosis of renal clear cell carcinoma

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Embodiment Construction

[0015] The present invention is further elaborated below by specific embodiment, it should be noted that, following description is only for explaining the present invention, and its content is not limited.

[0016] The method for obtaining the molecular marker of the present invention is as follows:

[0017] 1. Download 620 tRNA gene annotation information and their sequences from the GtRNAdb (http: / / gtrnadb.ucsc.edu / genomes / eukaryota / Hsapi19) database. Annotations and sequence information for newly composed CCA-tRNAs were obtained by adding "CCA" at the 3′ end of the mature tRNA sequence. Then, starting from the 5' or 3' end of the CCA-tRNAs sequence, all fragment sequences in the range of 15-30 nt were obtained. After further merging the same sequences, 8681 fragment sequences and 9285 fragment sequences from the 5' end of CCA-tRNAs were finally obtained. A fragment sequence from the 3′ end of CCA-tRNAs. In addition, we used the bedtools tool to obtain the annotation and s...

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Abstract

The invention discloses application of a novel molecular marker to the preparation of a kit for diagnosis and prognosis of renal clear cell carcinoma. The RNA sequence of the novel molecular marker isshown in SEQ ID NO: 1 to SEQ ID NO: 57. A mathematical model for diagnosis of renal clear cell carcinoma is constructed by taking the marker as a base; the model is high in sensitivity and good in specificity, AUC can be as high as 0.997, and the diagnosis effect is good. In addition, 57 tRF fragments can serve molecular markers for classifying renal clear cell carcinoma and predicating the survival periods of patients; in test data, a renal clear cell carcinoma tumor sample is clustered into 3 subtypes according to rRFs expression, and survivorship curve analysis shows that the survival periods of the subtypes have obvious difference. The novel tRF molecular marker has excellent diagnosis index characteristic, can be effectively applied to the diagnosis, classification and prognosis of renal clear cell carcinoma, and has high clinical application and promotion values.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a novel molecular marker and its application in the preparation of a kit for the diagnosis and prognosis of renal clear cell carcinoma. Background technique [0002] Renal cell carcinoma (renal cell carcinoma) is one of the most lethal tumors in the urinary system. It is a malignant tumor originating from the urinary tubular epithelial system of the renal parenchyma. It is also known as renal adenocarcinoma and is one of the most common tumors. Its incidence and The mortality rate accounts for about 2-3% of all tumors. Kidney clear cell renal carcinoma (KIRC) is the most common subtype of renal cancer, accounting for about 70-80% of renal cancer. Worldwide, approximately 102,000 patients die from it. Clear cell renal cell carcinoma is a tumor that develops relatively slowly, and its early symptoms often lack specificity. Many clear cell renal cell carcinomas are fo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G06F19/18
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178G16B20/00
Inventor 刘鹏渊王平孙喜伟陆燕周莉媛
Owner ZHEJIANG UNIV
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