Lung cancer DNA methylation molecular markers and application thereof in preparation of kit for early diagnosis of lung cancer

A technology for molecular markers and early diagnosis, which is applied in the determination/testing of microorganisms, biochemical equipment and methods, and biological systems, etc. Diagnosis and other issues, to achieve the effect of good specificity, good diagnostic index characteristics, and good diagnostic effect

Pending Publication Date: 2021-06-11
ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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Problems solved by technology

Non-small cell lung cancer proliferates and invades more slowly, making it less likely to be detected and diagnosed
When most patients with non-smal

Method used

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  • Lung cancer DNA methylation molecular markers and application thereof in preparation of kit for early diagnosis of lung cancer
  • Lung cancer DNA methylation molecular markers and application thereof in preparation of kit for early diagnosis of lung cancer
  • Lung cancer DNA methylation molecular markers and application thereof in preparation of kit for early diagnosis of lung cancer

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Embodiment Construction

[0019] The present invention will be further elaborated below through specific implementation examples. It should be noted that the following descriptions are only for explaining the present invention, and do not limit its content.

[0020] Such as figure 1 As shown, the method for obtaining the molecular markers of the present invention is as follows:

[0021] 1. Sequencing library construction and high-throughput sequencing: 18 samples of stage I non-small cell lung cancer were collected, and the total DNA in each patient's lung tumor tissue and its adjacent normal lung tissue samples was separated, and the genome was enzymatically analyzed with MspI. cutting; then perform end repair on the resulting DNA fragment; add base A to the 3' end after end repair; connect the methylated adapter to the sticky end A of the DNA fragment; then select a DNA fragment with a size of 40-220bp; select The fragments are treated with bisulfite, so that the non-methylated cytosine in the DNA f...

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Abstract

The invention discloses a group of DNA methylation molecular markers for lung cancer and application of the DNA methylation molecular markers in preparation of a kit for early diagnosis of lung cancer. The marker is obtained through methylation of 20 gene sequences of CDO1, SOX17, TCF21, TRIM58, ITGA9, CYYR1, CLEC14A, SLIT2, ZNF677, IRX2, ACVRL1, OSR1, ADCY8, GALNT13, HSPB6, IRX1, ITGA5, PCDH17, TBX5 and TTEX1D1. Eight of the gene sequences are methylated fingerprint genes newly found in lung cancer, and the methylated fingerprint genes comprise ADCY8, GALNT13, HSPB6, IRX1, ITGA5, PCDH17, TBX5 and TTEX1D1. On the basis of the markers, a mathematical model for lung cancer diagnosis is constructed; and the model is high in sensitivity and good in specificity, the AUC can reach 0.998, and the diagnosis effect is good. The invention also discloses a method for detecting the DNA methylation marker. The DNA methylation molecular marker disclosed by the invention has good diagnostic index characteristics, can be effectively used for lung cancer diagnosis, and has relatively high clinical use and popularization values.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a group of DNA methylation molecular markers of lung cancer and their application in preparing a diagnostic kit for lung cancer. Background technique [0002] Lung cancer is one of the biggest malignant tumors that threaten people's health and life. In 2020, about 1.8 million people will die of lung cancer in the world. . Lung cancer is mainly divided into two types: non-small cell lung cancer (NSCLC) and small cell lung cancer. NSCLC is the most important type, accounting for more than 80% of all lung cancers. NSCLC mainly includes two subtypes, adenocarcinoma and squamous cell carcinoma. Adenocarcinoma accounts for 40% of all lung cancer types and about 55% of NSCLC. Non-small cell lung cancer proliferates and invades slower, so it is more difficult to be detected and diagnosed. When most patients with non-small cell lung cancer are diagnosed, they have alread...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6809G16B25/20G16B20/30G16B5/00G16B40/00
CPCC12Q1/6886C12Q1/6809G16B25/20G16B20/30G16B5/00G16B40/00C12Q2600/154
Inventor 陆燕孙喜伟周莉媛刘鹏渊陈恩国
Owner ATTACHED OBSTETRICS & GYNECOLOGY OSPITAL MEDICALCOLLEGE ZHEJIANG UNIV
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