Novel molecular marker and application thereof in preparing kit for diagnosis and prognosis of head and neck cancer

Abstract

The invention discloses a novel molecular marker and application thereof in preparing a kit for diagnosis and prognosis of head and neck cancer. RNA (Ribonucleic Acid) sequences of the novel molecularmarker are shown as SEQ ID NO:1 to SEQ ID NO:48. A mathematical model for diagnosing the head and neck cancer is constructed by taking the marker disclosed by the invention as a basis, and has the advantages of high sensitivity, good specificity and good diagnosis effect; AUC can be as high as 0.981; besides, 48 tRF fragments can be used as molecular markers for typing the head and neck cancer and predicting life expectancy of patients; head and neck cancer tumor samples are clustered into four subtypes according to tRFs expression in test data; the analysis of a survival curve shows that thelife expectancies of the subtypes have significant difference. The novel tRF molecular marker disclosed by the invention has good characteristics of diagnostic indicators and higher clinical use andpopularization value, and can be effectively used for diagnosis, typing and prognosis of the head and neck cancer.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a novel molecular marker and its application in preparing a kit for diagnosis and prognosis of head and neck cancer. Background technique [0002] Head and neck cancer includes tumors originating from any tissue or organ in the head and neck except the eye, brain, ear, thyroid and esophagus, including neck tumors, ENT tumors, and oral and maxillofacial tumors (oral cancer). Since more than 90% of head and neck tumors are squamous cell carcinomas, head and neck cancers are also called head and neck squamous cell carcinomas (HNSC). Head and neck cancer is a malignant tumor with a high incidence in China and even the global cancer spectrum. It ranks sixth among common tumors. It has a high degree of malignancy and an average 5-year survival rate of less than 50%. After decades of development, surgery, radiotherapy and chemotherapy have not significantly improved the 5-...

AI Technical Summary

Problems solved by technology

After decades of development, surgery, radiotherapy and chemotherapy have not significantly improved the 5-year survival rate of head and neck cancer patients, and due to the special anatomical site, the resection range of head and neck cancer surgical treatment is severely limited, and the quality of life of patients after treatment generally declines , often disfiguring or disabling

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