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Monoclone antibody against human cardiac troponin I and its use

A cardiac troponin and monoclonal antibody technology, applied in the direction of anti-animal/human immunoglobulin, material inspection products, microorganisms, etc., can solve the problems of weak immunogenicity and increase the difficulty of monoclonal antibodies, and achieve specificity High and stable effect

Inactive Publication Date: 2005-07-27
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

cTnI is only weakly immunogenic, which increases the difficulty of preparing monoclonal antibodies

Method used

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Examples

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Embodiment Construction

[0022] The following examples will help those of ordinary skill in the art to further understand the present invention, but do not limit the present invention in any form.

[0023] 1. Immune mice: immunize BALB / c mice with human cardiac troponin I protein expressed and purified by laboratory genetic engineering, the first intraperitoneal injection, 50 μg each; the second intrasplenic immunization, each mouse 30μg; then use subcutaneous multi-point injection, 60μg per mouse. The serum antibody titer was measured by ELISA, and after reaching a certain value, a shock immunization was performed, and the splenocytes of the immunized mice were collected three days later for fusion.

[0024] 2. Preparation of myeloma cells: resuscitate SP2 / 0 tumor cells 7-10 days before cell fusion to ensure that the cells are in the logarithmic growth phase and have the best vitality when they are fused.

[0025] 3. Cell fusion: One day before fusion, 6-8 week-old BALB / c mice were killed by neck pu...

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PUM

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Abstract

This invention relates to a cTnI monocloned antibody and its hybridoma cell system. The said monocloned antibody has high specificity sensitivity and is easily prepared. It can identify different antigenic determinants on amino acid peptide segments 28-42, so as to be used for preparation of kits for diagnosis of cardiac infarction, angina pectoris and myocarditis.

Description

technical field [0001] The invention relates to a new anti-human cardiac troponin I (cTnI) monoclonal antibody and its application. Background technique [0002] Acute myocardial infarction (AMI) is a disease with acute onset and critical condition, which requires rapid and accurate laboratory diagnosis. The detection of cardiac troponin has the advantages of convenience, high sensitivity, strong specificity, and long duration. In 1987, Cummions et al first reported the use of radioimmunoassay to measure the concentration of cardiac troponin in peripheral blood to diagnose acute myocardial infarction. cTnI is only expressed in the myocardium, and its concentration in the blood increases, which highly specifically indicates myocardial injury. Therefore cTnI is a myocardial specific antigen. The sensitivity and specificity of cTnI in the diagnosis of AMI are higher than those of widely used markers such as CK-MB, and it is a new specific marker of myocardial injury. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18C12N5/18G01N33/53
Inventor 朱乃硕李瑞
Owner FUDAN UNIV
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