Preparing method of photoelectrochemical sensor based on sandwich cardiac troponin T marked by Ag2Se@CdSe and application

A cardiac troponin and photoelectrochemical technology, applied in the field of photoelectrochemical sensor preparation, can solve the problems of difficult application and low photoelectric conversion efficiency, and achieve the effects of high sensitivity detection, excellent photoelectric conversion characteristics, and increased sensitivity

Inactive Publication Date: 2015-08-19
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]TiO2 is a semiconductor material widely used in photocatalysis, solar cells and fuel cells, etc. However, due to TiO 2 wide bandgap (3.2 eV), it can only be effectively excited in the ultraviolet region, so the photoelectric conversion efficiency in the visible region is extremely low, and TiO2 nanoribbons have relatively low High electron-hole recombination efficiency, which brings certain difficulties to its application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0029](1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen.

[0030] (2) Add 6 μL, 4 mg / mL CeO dropwise 2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0031] (3) Add 6 μL, 8 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0032] (4) Drop 3 μL of BSA solution with a mass fraction of 0.5% on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0033] (5) Wash with ultra-pure water, dry to a wet state, apply 6 μL of cardiac troponin T ant...

Embodiment 2

[0035] Example 2 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0036] (1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen.

[0037] (2) Add 6 μL, 5 mg / mL CeO dropwise 2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0038] (3) Add 6 μL, 10 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0039] (4) Drop 3 μL of BSA solution with a mass fraction of 1% on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0040] (5) Wash with ultra-pure water, dry to a wet state, apply 6 μL of cardiac troponin T ant...

Embodiment 3

[0042] Example 3 An Ag-based 2 The preparation method of the photoelectrochemical sensor of the sandwich-type cardiac troponin T labeled with SeCdSe:

[0043] (1) Pretreatment of ITO electrodes: Cut the ITO conductive glass to a size of 2 cm × 0.5 cm, ultrasonically clean it with detergent, acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen.

[0044] (2) Add dropwise 6 μL, 6 mg / mL CeO 2 -TiO 2 The solution was placed on the surface of the electrode and dried at room temperature.

[0045] (3) Add 6 μL, 12 μg / mL cardiac troponin T capture antibody Ab 1 On the surface of the electrode, dry it in a refrigerator at 4°C, and rinse it with ultrapure water;

[0046] (4) Drop 3 μL of BSA solution with a mass fraction of 2.0% on the surface of the electrode to seal the non-specific active sites on the surface of the electrode, and dry it in a refrigerator at 4°C;

[0047] (5) Wash with ultra-pure water, dry to a wet state, apply 6 μL of cardiac troponin T a...

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PUM

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Abstract

The invention relates to a preparing method of a photoelectrochemical sensor based on sandwich cardiac troponin T marked by Ag2Se@CdSe and application, and belongs to the technical field of novel functional materials and biosensing detection. According to concrete contents of the preparing method and the application, CeO2-TiO2 composite nanometer materials with photoelectrocatalytic activity are used as optical activity substrate materials; Ag2Se@CdSe is used as an antibody marker; the sandwich photoelectrochemical sensor is prepared through the signal amplification effect of the marker Ag2Se@CdSe on the substrate materials, and is used for the high-sensitivity detection of the cardiac troponin T of myocardial damage specific marker. The method has the important significance on the early diagnosis and treatment of acute myocardial infarction.

Description

technical field [0001] The present invention relates to an Ag-based 2 Preparation method and application of SeCdSe-labeled sandwich cardiac troponin T photoelectrochemical sensor. specifically CeO 2 -TiO 2 Composite nanomaterials as photoactive substrate materials, with Ag 2 SeCdSe is an antibody label, through the label Ag 2 The invention relates to the signal amplification effect of SeCdSe on a base material, and prepares a sandwich-type photoelectrochemical sensor for detecting cardiac troponin T, which belongs to the technical field of new functional materials and biosensing detection. Background technique [0002] Photoelectrochemical detection is an emerging bioanalytical detection method. In the photoelectrochemical detection process, light is the excitation signal, and the electrical signal is the detection signal. The background signal is reduced due to the complete separation of the excitation signal and the detection signal, so that photoelectrochemistry has ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26G01N27/327G01N33/68G01N33/543G01N33/58
Inventor 柳懿芯魏琴马洪敏范大伟王晓东胡丽华杜斌曹伟李贺吴丹
Owner UNIV OF JINAN
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