39 results about "Hucho taimen" patented technology

The invention provides a feedstuff for sturgeon and hucho taimen at mouth - open period, which solves the problems of the conventional feedstuff that the growth rates of the sturgeon and hucho taimen are slow and the survival rates are low. The feedstuff comprises: dextrin, full cream milk powder, blood powder, white fish meal, soy protein, L-alanyl-L-glutamine, methylol cellulose and ore-mixing agent. When the feedstuff is used the for sturgeon and hucho taimen at mouth - open period, the growth rates and the survival rates of the sturgeon and hucho taimen are increased and the special growth rates of the sturgeon and hucho taimen are 1.5%-6.2% and the survival rates are more than 98%.

An artificial culture and domestication method for a fingerling of Hucho taimen relates to the aquatic product culturing field which solves the problem that the current culturing technology is not suitable for the fingerling of Hucho taimen and can be finished by the following four phases: 1. cleaning a hatching slot 2 to 10 times a day and picking out dead eggs, dead fingerling and egg skin; 2. feeding zooplanktons for 4 to 7 days and 6 to 8 times one day; 3. feeding the zooplanktons and water earthworms for the first 3 to 5 days; then feeding the water earthworms for 4 to 5 times a day for the next one week; then feeding massive materials once every morning for three weeks and feeding the water earthworms in the daytime and at night for 4 to 5 times a day; 4. feeding artificial feedstock in the morning and massive materials in the daytime for 2 to 3 times a day and the water earthworms at night during the first 2 to 3 weeks; and feeding the artificial feedstock for 3 to 4 times a day by every 2.5 to 3 hours for one time and feeding the water earthworms at night after the next 2 to 4 weeks; feeding the artificial feedstock four times a day for the last 15 days and finishing the domestication work. The domestication method of the invention has the advantages of simple and convenient operation, low cost and good domestication fingerling quality.

A method for rearing hybrid salmon seeds is used for solving the problems that presently, death rate of hybrid salmon seeds obtained by mating Brachymystax lenok with taimen is high, and techniques of rearing seeds of other salmon species is highly complex and difficult to industrialize and is unsuitable for rearing hybrid seeds of Brachymystax lenok and taimen. The method includes: placing fertilized eggs of hybrid salmon into sterilized parallel tanks, incubating the fertilized eggs in the shade till fry begin hatching, and removing egg skin, dead eggs and dead fry during hatching; and using water fleas as biological bait for feeding when hybrid salmon fry open mouths, using Tubificidae to feed the fry on the seventh day to the tenth day after mouth opening, mixing Tubificidae with complete feed into muddy feed for feeding, and using the complete feed to feed the fry. Hybrid salmon seeds reared by the method are high in quality, and the survival rate can be increased to more than 80%.

The invention discloses a mitochondrion complete genome sequence of hucho taimen and amplification primers of the mitochondrion complete genome sequence, which relate to the mitochondrion complete genome sequence and the amplification primer of the mitochondrion complete genome sequence. The mitochondrion complete genome sequence of the hucho taimen determined by the invention provides a material basis for conservation genetics, evolutionary genetics and molecular marker assisted selection of good breeding species of the hucho taimen. The mitochondrion complete genome sequence of the hucho taimen is as shown by SEQ ID NO:1. The mitochondrion complete genome sequence of the hucho taimen comprises 33 pairs of the amplification primers. The mitochondrion complete genome sequence of the hucho taimen and the amplification primers of the mitochondrion complete genome sequence provide the material basis for the conservation genetics, the evolutionary genetics and the molecular marker assisted selection of the good breeding species of the hucho taimen.

The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.

A special feedstuff used for hucho taimen larvae relates to a fish feedstuff. The invention solves the problems of low growth speed and high death rate of hucho taimen by feeding the hucho taimen withrainbow trout feedstuff. The hucho taimen special feedstuff comprises the following components in weight percentage: 2-5 percent beer yeast, 2-6 percent wheat-middling, 2-8 percent spray drying bloodflour, 60-70 percent fish flour, 10-20 percent fish oil, 1-4 percent soybean lecithin, 0.2-0.8 percent sodium alginate, 0.01-0.05 percent L-carnitine, 0.05-0.2 percent KCL, 0.02-0.1 percent inositol,0.02-0.07 percent enzyme preparation, 0.01-0.04 percent high stability VC, 0.1-0.5 percent magnesite, 0.01-0.05 percent antioxidant, 0.05-0.02 percent Kemei, 0.8-1.5 percent zeolite. Compared the special feedstuff with the imported rainbow trout feedstuff, by feeding the hucho taimen with the special feedstuff, the survival rate of the hucho taimen can reach above 95% after 4 weeks, and the survivalrate of the hucho taimen can reach above 92% after 8 weeks; the average weight is increased by 18.35%, and the weight gain rate is improved by 9.49%.

Provided is a factory-like recirculating aquaculture system for Hucho taimen, pertaining to the technical field of aquaculture. The key points of the system are described in that outlet ends of aquaculture ponds communicate with a sedimentation pond through pipelines; the sedimentation pond is located on the side of the aquaculture ponds; a microfiltration machine pond is arranged on the side of the aquaculture pond; additionally, an outlet end of the sedimentation pond communicates with an inlet end of the microfiltration machine pond; the side of the microfiltration machine pond is equippedwith a water return pond; aquaculture water at an outlet end of the microfiltration machine pond and an inlet end of the water return pond as well as in the water return pond enter a floating ball-type biological filter through a pipeline and a water pump; an outlet of the floating ball-type biological filter communicates with a trickling filter through a pipeline and a water pump; the trickling filter is located above an oxygenating tank; the oxygenating tank is disposed on the side of the water return pond; the interior of the oxygenating tank is equipped with an ultraviolet radiation disinfection device; the ultraviolet radiation disinfection device communicates with the oxygenating tank; water in the oxygenating tank communicates with the aquaculture ponds through pipelines. The factory-like recirculating aquaculture system for Hucho taimen is used for factory-like aquaculture of Hucho taimen.

The invention relates to a factory-like circulating water breeding method for hucho taimen, and belongs to the technical field of aquaculture. The method is characterized in that a hucho taimen circulating water breeding system is connected with a hucho taimen juvenile fish breeding system, juvenile fishes are subjected to tameness and cultivation and parallel screening in sequence, fish fry of different sizes are put into multiple breeding ponds respectively, water drained from the breeding ponds passes through a settling pond and a micro filter tank, and then residual feed and fecal suspensions are filtered and removed; detection is conducted by means of a PH online monitoring system, and the PH value of breeding water is adjusted; the breeding water enters a floating type biofilter through a water pump to remove organic matter such as ammonia nitrogen, nitrate and nitrite; harmful gas such as CO2 is removed in a trickling filter; pure oxygen is increased in an oxygen increasing groove; water obtained after oxygen increasing is subjected to ultraviolet ray disinfection; finally, the breeding water automatically flows into the breeding ponds through an oxygen increasing high-levelwater tank, and the whole hucho taimen circulating water breeding process is completed. The method is used for factory-like hucho taimen breeding.

The invention discloses methods for acquiring a micro-satellite sequence and polymorphic micro-satellite markers of hucho taimen and the polymorphic micro-satellite markers of the hucho taimen, which relate to a method for acquiring the micro-satellite sequence and the polymorphic micro-satellite markers and the polymorphic micro-satellite markers. The method for acquiring the micro-satellite sequence comprises the following steps: firstly, extracting a hucho taimen genome DNA; secondly, performing enzyme cutting on the hucho taimen genome DNA and constructing a micro-satellite enrichment library; and thirdly, performing colony PCR amplification and performing positive clone detection and sequencing. The method for acquiring the polymorphic micro-satellite markers of the hucho taimen comprises the following steps: performing the first step to the third step the same as that of the method for acquiring the micro-satellite sequence; fourthly, analyzing the micro-satellite sequence of the hucho taimen and designing a primer; and fifth, performing polymorphic identification on the primer of the micro-satellite sequence. The method acquires seven pairs of the polymorphic micro-satellite markers of the hucho taimen. The biological materials obtained by the methods can be used for conservation genetics, genetic relationship analysis, linkage map construction and genetic management of cultured populations of the hucho taimen.

The invention discloses a fresh and alive Hucho taimen big fish transporting method. The method includes the following steps of firstly, preparing a container for containing fresh and alive Hucho taimen big fish, wherein the volume of the container ranges from 100 L to 300 L; secondly, adding one volume of water to the container, placing a fresh and alive Hucho taimen big fish in the container so that the body of the big fish is totally immersed in water; thirdly, sealing the opening after 5 volumes to 6 volumes of pure oxygen is injected into the container; fourthly, transporting the big fish through a bubble chamber at a low temperature. The fresh and alive Hucho taimen big fish transporting method can solve the problem that the death rate is high during Hucho taimen big fish transportation.

The invention discloses a method for inducing hucho taimen young fishes to open mouths and to be domesticated. The method includes the steps: stabilizing the young fishes for 1-2 days and then pouring the young fishes into a disinfected hatching tank by a bolting silk after the young fishes completely float in a floating management period; mixing trout feed and krill powder according to the ratio of 2-3:1, feeding the young fishes for 4-5 days, changing the mixing ratio into 3-4:1, feeding the young fishes for 3-4 days, changing the mixing ratio into 6-8:1 and feeding the young fishes for 2-3 days in a food calling and feeding period; feeding the young fishes with 0.6-0.8mm trout feed for 3-4 days at a first stage, training the young fishes in scrambling for taking food, feeding the young fishes for 3-4 days at a second stage and training the food intake rhythm of the young fishes scrambling for pure total artificial feed in a total artificial feed feeding period. The method can solve the problem that large-scale domestication of the hucho taimen young fishes lack easily preserved conventional baits with a wide source and high palatability and without pathogenic pollution.

The invention provides a feed additive for freshwater fishes and a preparation method of the feed additive. The feed additive is prepared from, by weight, 10-15 parts of radix angelica sinensis, 5-10 parts of cyrtomium rhizomes, 8-12 parts of medicated leaven, 6-8 parts of hawthorn fruits, 5-8 parts of dried tangerine or orange peel and 8-15 parts of composite microbial thalli. The preparation method comprises the following steps that 1, radix angelica sinensis, cyrtomium rhizomes, medicated leaven, hawthorn fruits and dried tangerine or orange peel are weighed according to the compatibility quantity to serve as raw materials in the group A, the raw materials in the group A are mixed and ground into crude powder, water is added according to 10-20 times of the weight of the medicinal materials, extraction is carried out at the temperature of 95 DEG C to 100 DEG C, filtering is carried out to obtain medicine liquid, and the medicine liquid is concentrated to 1:1 to obtain extract; 2, the composite microbial thalli are weighed according to the compatibility quantity, the extract in the step 1 and the composite microbial thalli are mixed and stirred to be uniform, pile turning fermentation is carried out for 2-3 days, a fermentation product is obtained, and the fermentation product is crushed to obtain the feed additive. According to the feed additive, the weight of Hucho taimen can be greatly increased, and the Hucho taimen cannot die after being cultured for a long time.

The invention discloses a complete extruded formula feed for Hucho taimen and a preparation method thereof. The complete extruded formula feed is made from the following raw materials: fish meal, cottonseed protein powders, fermented bean cake, bread flour, beer yeast, calcium dihydrogen phosphate, a vitamin-mineral salt mixture, choline chloride, dimethylpropiothetin (DMPT), or a cotton cake/rape cake mixture, wherein the cotton cake/rape cake mixture is composed of cotton cake and rape cake at a weight ratio of 1:1. The preparation method of the complete extruded formula feed for Hucho taimen comprises the steps as follows: roughly grinding large-sized raw materials to coarse powders, mixing all materials, micronizing to powders which can pass through a 100-mesh sieve, compounding, extruding to make pellets, and oven-drying. The preparation method is simple and practical and is favorable to industrial production. The complete extruded formula feed for Hucho taimen has the advantages of good palatability, high domestication speed, lower feed coefficient and good stability.

The invention relates to a molecular marker for identifying genetic sex of fishes and an identifying method, in particular to a molecular marker for identifying genetic sex of hucho taimen and an identifying method. A nucleotide sequence of specific stripes of male hucho taimen in the molecular marker for identifying genetic sex of hucho taimen is shown in SEQ ID NO:1, and the nucleotide sequenceof common stripes of male hucho taimen is shown in SEQ ID NO:2. The indentifying method comprises the steps as follows: 1, extraction of genomic DNA; 2, PCR amplification; 3, gel electrophoresis: hucho taimen showing one stripe is female while hucho taimen showing two stripes is male. By use of the method, the sex of juvenile hucho taimen can be rapidly and efficiently identified, only a pair of primers is needed for amplification, the sex of the hucho taimen can be identified without sequencing according to the number of stripes of an agarose gel electrophoresis amplified product.

The invention discloses a hucho taimen seminal fluid ultralow-temperature cryopreservation fluid and a hucho taimen seminal fluid cryopreservation method, and relates to a fish seminal fluid ultralow-temperature cryopreservation fluid and a hucho taimen seminal fluid cryopreservation method, which are used for solving the problems that in an existing hucho taimen sperm long-term freezing technique, the motility rate is low, viability of alive sperms is low, and the activity ratio is reduced greatly. In the hucho taimen seminal fluid ultralow-temperature cryopreservation fluid, the molar ratio of K<+> to Na<+> is smaller than 1 to 16, and the concentration of K<+> in the hucho taimen seminal fluid ultralow-temperature cryopreservation fluid is 0.00480mol/L to 0.00682mol/L. The cryopreservation method comprises the following steps: firstly, collecting fresh hucho taimen seminal fluids; secondly, preparing a seminal cryopreservation mixed solution; thirdly, subpackaging the seminal fluid cryopreservation mixed solution; fourthly, performing cryopreservation; and fifthly, unfreezing by adopting a rapid water bath unfreezing method. The method is simple and feasible, and has the advantages of long cryopreservation time, high sperm quality after unfreezing.

The invention relates to a method for obtaining high-quality Hucho taimen sperms through screening. The problems that the fertilization rate and the hatching rate are low, and the malformation rate is high in the actual production process due to the fact that the sperm quality cannot be determined in the prior art are solved. The method comprises the steps that (1) the content of Se and Ze in seminal fluid and testosterone in serums of Hucho taimen is measured; (2) seminal fluid of the male Hucho taimen with serums with testosterone being larger than 4.9175 microgram/mg and smaller than 12.4951 microgram/mg is selected, wherein the content of Se in the seminal fluid is larger than 0.1024 microgram/mg and the content of Ze in the seminal fluid is larger than 11.8725 microgram/mg; the high-quality Hucho taimen sperms are obtained. The high-quality Hucho taimen sperms obtained through screening according to the method are high in fertilization rate and hatching rate, low in malformation rate, the artificial Hucho taimen feeding efficiency is greatly improved, and the feeding cost is saved.

The invention discloses a primer pair, reagent kit and method for distinguishing brachymystax lenok from Hucho taimen. The primer pair comprises a primer pair 1 and a primer pair 2, wherein the primerpair 1 comprises an upstream primer ITS-F and a downstream primer ITS-R, and the primer pair 2 comprises an upstream primer mtDNA-F and a downstream primer mtDNA-R; the sequence of the ITS-F is shownas SEQID NO.1, the sequence of the ITS-R is shown as SEQID NO.2, the sequence of the mtDNA-F is shown as SEQID NO.3, and the sequence of the mtDNA-R is shown as SEQID NO.4. A new method for distinguishing brachymystax lenok from Hucho taimen based on a molecular biology technique principle is adopted for the first time at home and abroad, 1 pair of specific primers is designed by using ITS genesand mitochondrial genome of the brachymystax lenok and the Hucho taimen, and a new method for identifying the brachymystax lenok and the Hucho taimen can be realized by a PCR reaction. The primer pair, the reagent kit and the method can be used for distinguishing the brachymystax lenok from the Hucho taimen complete individual and processed products thereof, and have the characteristics of being accurate, quick, objective, fair, economical and practical.

The invention relates to a microsatellite marker, a primer and an identification method for genetic sex identification of hucho taimen and an application thereof. The nucleotide sequence of the microsatellite marker is as shown in SEQ ID NO: 1. The method for identifying the hereditary sex of hucho taimen comprises the following steps: collecting fin ray samples of female and male hucho taimen; extracting genomic DNA of the fin ray sample, and carrying out PCR amplification; and carrying out electrophoresis detection on the obtained PCR amplification product; wherein when one strip of 208bp is displayed through electrophoresis, the Hucho taimen is female, and when strips of 208bp and 331bp2 are displayed, the Hucho taimen is male. The microsatellite marker and the identification method can accurately and efficiently identify the sex of the hucho taimen, fish killing is not needed, the method is simple and rapid, tedious sequencing steps are omitted, the identification accuracy is improved, and the microsatellite marker and the identification method can also be used for early sex identification of the hucho taimen.

The invention discloses a hucho taimen trapping device, which comprises a fish trapping box enclosed by mesh walls, wherein a sliding door is arranged on the middle part of the mesh wall at the front end of the fish trapping box; a sliding door rope is connected to the middle part of each of the two ends of the sliding door; the sliding door can slide to and fro to be opened and closed under the action of the sliding door ropes; a fish storage net bag is connected to the rear end of the mesh wall at the rear end of the fish trapping box, and is fixedly connected through upper and lower net ropes, and an internal space of the fish storage net bag is supported by two steel bar support rings until the fish storage net bag extends to a zipper bag pulling stake; a fish inlet of the fish storage net bag is formed in the middle part of the mesh wall at the rear end of the fish trapping box; fish inlet barbs are connected to the fish storage net bag, and internally extend to be connected with a fish inlet barb opening which is fixed by a fish inlet barb opening tie; a fish trapping bag is arranged on the middle part of the fish trapping box, and is fixed by a fish trapping bag tie; the fish trapping box, the sliding door and the fish storage net bag are fixedly connected through the mesh walls, upper and lower net ropes, set stakes and the bag pulling stake.

The invention provides a preparation method of a Hucho taimen triploid fry. The preparation method comprises: performing triploid heat shock induction, specifically, 15 min after fertilizing a diploidoosperm, putting the oosperm into a water bath at 17 DEG C for transition for 1 min, then putting the oosperm into a water bath at 26-27 DEG C for heat treatment for 15 min and then putting the oosperm into a water bath at 17 DEG C for transition for 1 min; incubating the oosperm, i.e., putting the oosperm subjected to triploid heat shock induction into a container at an incubation water temperature of 7-9 DEG C for incubation. By adopting the method, after 30- 40 days, the Hucho taimen triploid fry can be obtained; during incubation, the eyed rate of the oosperm is 80% or more, the incubation rate is 70% or more, and the triploid rate is up to 90% or more. The method provided by the invention effectively alleviates the problems of reduced growth rate after sexual maturity, reduced fullness, high mortality after broodstock reproduction and the like, improves disease resistance, and is suitable for producing large-sized fish for preparing sashimi, has the advantages of low cost, easiness in operation and the like, and has good market value.