Hucho taimen seminal fluid ultralow-temperature cryopreservation fluid and hucho taimen seminal fluid freezing and preserving method

Abstract

The invention discloses a hucho taimen seminal fluid ultralow-temperature cryopreservation fluid and a hucho taimen seminal fluid cryopreservation method, and relates to a fish seminal fluid ultralow-temperature cryopreservation fluid and a hucho taimen seminal fluid cryopreservation method, which are used for solving the problems that in an existing hucho taimen sperm long-term freezing technique, the motility rate is low, viability of alive sperms is low, and the activity ratio is reduced greatly. In the hucho taimen seminal fluid ultralow-temperature cryopreservation fluid, the molar ratio of K<+> to Na<+> is smaller than 1 to 16, and the concentration of K<+> in the hucho taimen seminal fluid ultralow-temperature cryopreservation fluid is 0.00480mol / L to 0.00682mol / L. The cryopreservation method comprises the following steps: firstly, collecting fresh hucho taimen seminal fluids; secondly, preparing a seminal cryopreservation mixed solution; thirdly, subpackaging the seminal fluid cryopreservation mixed solution; fourthly, performing cryopreservation; and fifthly, unfreezing by adopting a rapid water bath unfreezing method. The method is simple and feasible, and has the advantages of long cryopreservation time, high sperm quality after unfreezing.

Description

technical field [0001] The invention relates to a low-temperature cryopreservation solution of fish semen and a cryopreservation method of jelly salmon semen. Background technique [0002] Salmonidae belongs to the family Salmonidae and belongs to the genus Salmonidae; the endangered level is Vulnerable. Due to its rich nutrition, tender meat and delicious meat taste, Jerome salmon is favored by consumers; therefore, the market demand for Jerome salmon has been increasing in recent years. Although the commercial farming and breeding of jelly salmon has been vigorously developed, the protection of germplasm resources has been neglected in the selection and breeding work, resulting in the inbreeding germplasm degeneration intensified in the process of jelly salmon hybrid breeding, genetic diversity Sexuality is reduced, quality is reduced, growth performance is low, and the defense ability of most aquaculture species to disease and environmental stress is reduced, and disease...

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of low viability, low viability of surviving sperm and a sharp decline in the activity rate of the existing jelly salmon sperm long-term freezing technology, and to provide a jelly salmon semen ultra-low temperature freezing preservation solution and z The cryopreservation method of salmon semen