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Method for fast and physically distinguishing quality of mature ova of hucho taimen

A technology of mature eggs and physical identification, which is applied in the field of physical evaluation of egg quality in Temendello, can solve the problem of increased mortality of seedlings with ruptured membranes, abnormal rate of seedlings, increased mortality of seedlings, inability to achieve early, rapid and accurate evaluation, fertilization rate, Hatch rate reduction and other issues

Inactive Publication Date: 2015-06-10
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the evaluation method of the mature ovum in the existing Taimen Zhe salmon breeding process is intuitively felt by the operator based on experience, and cannot realize early, fast and accurate evaluation, thereby resulting in a decrease in fertilization rate and hatching rate. Due to the technical problems of increased deformity rate of membrane-ruptured seedlings and increased mortality of floating seedlings, a method for quickly physically distinguishing the mature eggs of Taimenzhe salmon is provided.

Method used

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  • Method for fast and physically distinguishing quality of mature ova of hucho taimen

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specific Embodiment approach 1

[0015] Specific implementation mode one: a kind of method of fast physical discrimination Taimenzhe salmon mature egg of this embodiment is carried out according to the following steps:

[0016] 1. Add the egg buffer cleaning solution for salmon into the clean spawning basin;

[0017] 2. Anesthetize the sexually mature female broodstock of Taimenzhe salmon, extrude the mature eggs from the abdominal cavity of the fish into the spawning basin filled with the egg buffer cleaning solution for salmon, and put only one mature broodstock in one spawning basin Eggs, then place the spawning basin in a ventilated and dark place;

[0018] 3. Randomly take out several fish eggs from the spawning basin, put them into clear water and stand still for 25-30 minutes, measure the weight and diameter of a single fish egg, and then calculate the average weight of a single fish egg X 1 and standard deviation SV 1 , the mean value of single egg diameter X 2 and standard deviation SV 2 ;Press C...

specific Embodiment approach 2

[0023] Specific embodiment two: the difference between this embodiment and specific embodiment one is: the salmon ovum buffer cleaning solution described in step one is prepared according to the following method: add NaCl 8.0g, KCl 0.3g, CaCl to 1 liter of distilled water successively 2 0.3g, NaHCO 3 0.04g, stirred until dissolved, to obtain salmon ovum buffer cleaning solution. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0024] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: single fish roe weight, average value of fish roe weight X 1The measurement method is as follows: randomly select fish eggs from the tested fish eggs to form n groups, m eggs in each group, blot the moisture of the eggs with filter paper, and then put the m eggs of group i into the analytical balance Measure and record the total weight G of eggs in each group i , and then according to the weight of a single fish egg A i =G i / m to calculate the weight A of a single fish egg of the i group i ; Wherein i=1, 2, 3, ..., n; the average weight of a single fish egg X 1 =(A 1 +A 2 +...+A i +...+A n ) / n. Others are the same as those in Embodiment 1 or 2.

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Abstract

The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.

Description

technical field [0001] The invention relates to a physical evaluation method for the quality of Temenjero's ovum. Background technique [0002] Taimen Salmon belongs to Salmonisdae and Salmon belongs to Hucho. Taimenzhe salmon are mainly distributed in Russia, China, Mongolia and Japan. At present, Zheluo salmon is endangered in my country, and only a small number of breeding populations survive in Wusuli River, Heilongjiang and Hanas Lake in Xinjiang. As a typical representative of the indigenous cold-water fishes in my country, Taimenzhe salmon is of great significance and social value in terms of the protection of germplasm resources and the consideration of breeding benefits. Taimenzhe salmon is the only large-scale fish among the few indigenous salmonids in my country. It is a rare and valuable species. After research, Taimenzhe salmon has been successfully artificially propagated, and large-scale domestication of larvae eating artificial feed has been realized. Afte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00G01B11/08G01G17/00G01N21/00
CPCY02A40/81
Inventor 张永泉尹家胜张颖佟广香郭文学
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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