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Method for fast and physically distinguishing quality of mature ova of hucho taimen

A mature egg, physical identification technology, applied in the field of physical evaluation of the egg quality of Taimenzello, can solve the problem of inability to achieve early, rapid and accurate evaluation, abnormal rate of ruptured seedlings, increased seedling mortality, fertilization rate, Problems such as reduced hatchability

Inactive Publication Date: 2014-02-19
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention aims to solve the problem that the evaluation method of the mature ovum in the existing Taimen Zhe salmon breeding process is intuitively felt by the operator based on experience, and cannot realize early, fast and accurate evaluation, thereby resulting in a decrease in fertilization rate and hatching rate. Due to the technical problems of increased deformity rate of membrane-ruptured seedlings and increased mortality of floating seedlings, a method for quickly physically distinguishing the mature eggs of Taimenzhe salmon is provided.

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  • Method for fast and physically distinguishing quality of mature ova of hucho taimen

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specific Embodiment approach 1

[0015] Specific embodiment one: a kind of quick physical method of the present embodiment to distinguish the quality of the mature eggs of Taimen zebra salmon is carried out according to the following steps:

[0016] 1. Add the salmon egg buffer wash solution to the clean spawning basin;

[0017] 2. Anesthetize the sexually mature female broodstock of Taimen zebra salmon, extrude the mature eggs from the abdominal cavity of the fish into the spawning basin filled with the egg buffer cleaning solution for salmon, and place only one mature broodstock in one spawning basin. eggs, and then place the spawning pot in a ventilated place away from light;

[0018] 3. Randomly take out a few fish eggs from the spawning basin, add them to the clear water for 25-30 minutes, measure the weight and diameter of a single fish egg, and then calculate the average value of the single fish egg weight X 1 and standard deviation SV 1 , the mean of the diameter of a single fish egg X 2 and standa...

specific Embodiment approach 2

[0023] Embodiment 2: The difference between this embodiment and Embodiment 1 is: the egg buffer cleaning solution for salmon described in step 1 is prepared in the following manner: NaCl8.0g, KCl0.3g, CaCl8. 2 0.3g, NaHCO 3 0.04g, stir until dissolved, and obtain the egg buffer cleaning solution for salmon. Others are the same as the first embodiment.

specific Embodiment approach 3

[0024] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the weight of a single fish egg, the average value of fish egg weight x 1The measurement method is as follows: randomly select fish eggs from the tested fish eggs to form n groups, each group of m eggs, dry the water in the eggs with filter paper, and then put m eggs in the i group on the analytical balance Measure and record the total weight G of the eggs in each group i , and then according to the weight of a single fish egg A i =G i / m Calculate the weight A of a single fish egg in the i-th group i ; where i=1, 2, 3, ..., n; the average weight of single fish eggs X 1 = (A 1 +A 2 +……+A i +……+A n ) / n. Others are the same as in the first or second embodiment.

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Abstract

The invention discloses a method for fast and physically distinguishing the quality of mature ova of hucho taimen and relates to a physical evaluation method for the quality of the ova of hucho taimen. The method aims to solve the technical problems that in the existing multiplicative process of hucho taimen, the mature ova are evaluated by operators through intuitionistic senses from experience, the ova of hucho taimen cannot be evaluated at the early stage fast and accurately, consequently, the fertility rate and the hatchability decrease, and the membrane rupture offspring seed malformation rate and the floating offspring seed death rate increase. According to the method, the pubescent female parent fishes of hucho taimen are narcotized, the mature ova are extruded to a spawning basin containing an ovum buffer cleaning solution for trout from the abdominal cavities of the fish bodies, the average weight of the fish ova and the dispersion coefficient of the average weight, the average diameter of the fish ova and the dispersion coefficient of the average diameter and the average distribution density of fat droplets of the fish ova are measured, judgment is made on the basis of the physically-measured values to divide the fish ova into the superior grade, the medium grade and the inferior grade, the judgment can be completed in 1.5h, and the method has the advantage of being capable of being used for distinguishing the quality of the ova conveniently at the early stage. The method can be used in the field of aquaculture of hucho taimen.

Description

technical field [0001] The invention relates to a physical evaluation method for the quality of Taimenzello eggs. Background technique [0002] The genus Salmonisdae, the genus Salmonisdae, and the genus Hucho. Taimen salmon is mainly distributed in Russia, China, Mongolia and Japan. At present, Zheluo salmon is endangered in my country, and only a small number of breeding groups survive in the Ussuri River, Heilongjiang and Hanas Lake in Xinjiang. As a typical representative of my country's indigenous cold-water fish, Taimen salmon is of great significance and social value in terms of protection of germplasm resources and breeding benefits. Taimen trout is the only large fish among the few indigenous salmonids in my country. It is a rare and precious species. After research, the artificial breeding of Taimen trout has been successful, and the large-scale domestication of larvae feeding artificial feed has been realized. After more than 10 years of research and promotion,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/00G01B11/08G01G17/00G01N21/00
CPCY02A40/81
Inventor 张永泉尹家胜张颖佟广香郭文学
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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