Microsatellite marker, primer and identification method for genetic sex identification of hucho taimen and application of microsatellite marker and primer for genetic sex identification of hucho taimen

A technology of microsatellite markers and salmonids, applied in the fields of microsatellite markers and primers and applications, can solve the problems of identifying sex by marriage color, failing to fully understand the sex ratio, and inconspicuous marriage color of broodstock, so as to save the sequencing step. Effect

Pending Publication Date: 2021-06-01
HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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Problems solved by technology

Sexually mature Jero salmon have marriage color only during the breeding season, the tail of the fish body is red, and the marriage color of some parent fish is not obvious, and the marriage color of Jelo salmon in different waters is also different, so it is difficult to identify gender by marriage color
[0003] Zheluo salmon is a

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  • Microsatellite marker, primer and identification method for genetic sex identification of hucho taimen and application of microsatellite marker and primer for genetic sex identification of hucho taimen

Examples

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Embodiment 1

[0029] Example 1 A Microsatellite Marker and Primers for Accurately Identifying the Genetic Sex of Salmon

[0030] A microsatellite marker for accurately identifying the genetic sex of salmon salmon, the nucleotide sequence of the microsatellite marker is shown in SEQ ID NO:1. The repeating unit is GGGTTA, and the core sequence is (GGGTTA) 16 GGTTA (GGGTTA) 5 GGTTA (GGGTTA) 7 .

[0031] A microsatellite marker primer for accurately identifying the genetic sex of salmon salmon, the upstream primer of the microsatellite marker is: 1F 5'-CACTGGGGTACAAAAAGAGCAA-3' (SEQ ID NO: 2);

[0032] The downstream primer is: 1R 5'-ACGGGCCTTTTTAAGCACT-3' (SEQ ID NO: 3).

Embodiment 2

[0033] Embodiment 2 utilizes microsatellite markers to identify the method for the genetic sex of jellyfish

[0034] A method for accurately identifying the genetic sex of jellyfish, comprising the following steps:

[0035] (1) Collect samples of female and male salmon, cut the fin rays and store them in ethanol with a volume content of 95%, replace the ethanol every 24 hours, store at -20°C, and store them for later use;

[0036] (2) extract the genomic DNA of the female and male fin ray samples of jellyfish, measure the DNA concentration with an ultraviolet spectrophotometer, dilute the DNA concentration to 50ng / L, and set aside;

[0037] (3) performing PCR amplification with the genomic DNA obtained in step (2) as a template;

[0038] The reaction system for PCR amplification was 25 μL, and 4 concentration gradients (1 μmol / L, 2 μmol / L, 5 μmol / L, and 10 μmol / L) were set for 18s with reference to the primers for PCR amplification; the 25 μL amplification system included 2×P...

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Abstract

The invention relates to a microsatellite marker, a primer and an identification method for genetic sex identification of hucho taimen and an application thereof. The nucleotide sequence of the microsatellite marker is as shown in SEQ ID NO: 1. The method for identifying the hereditary sex of hucho taimen comprises the following steps: collecting fin ray samples of female and male hucho taimen; extracting genomic DNA of the fin ray sample, and carrying out PCR amplification; and carrying out electrophoresis detection on the obtained PCR amplification product; wherein when one strip of 208bp is displayed through electrophoresis, the Hucho taimen is female, and when strips of 208bp and 331bp2 are displayed, the Hucho taimen is male. The microsatellite marker and the identification method can accurately and efficiently identify the sex of the hucho taimen, fish killing is not needed, the method is simple and rapid, tedious sequencing steps are omitted, the identification accuracy is improved, and the microsatellite marker and the identification method can also be used for early sex identification of the hucho taimen.

Description

technical field [0001] The invention relates to the identification of the genetic sex of the salmon, in particular to a microsatellite marker, a primer and an application for accurately identifying the genetic sex of the salmon. Background technique [0002] Hucho taimen (pallas) belongs to the order Salmoniformes, the family Salmonidae, and the genus Hucho. It is a rare and valuable cold-water fish in my country, with tender meat and delicious taste. Zheluo salmon is the largest fish in the family Salmonidae. It grows rapidly and has no secondary sexual characteristics when it is sexually mature. In the wild, female fish are sexually mature at 5 to 6 years old, and male fish are sexually mature at 4 to 5 years old. Sexually mature Jero salmon have marriage color only during the breeding season, and the tail of the fish body is red, and the marriage color of some parent fish is not obvious. The marriage color of Jelo salmon in different waters is also different, and it is di...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6879C12Q1/686C12N15/11
CPCC12Q1/6888C12Q1/6879C12Q1/686C12Q2600/156C12Q2525/151Y02A40/81
Inventor 唐国盘周晶路畅王新华张潇刘晓晓赵文科张士彪张福圆武文一郭国军徐文彦齐子鑫段嘉段硕硕任慧格程雅惠
Owner HENAN UNIV OF ANIMAL HUSBANDRY & ECONOMY
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