Microsatellite marker, primer and identification method for genetic sex identification of hucho taimen and application of microsatellite marker and primer for genetic sex identification of hucho taimen

Abstract

The invention relates to a microsatellite marker, a primer and an identification method for genetic sex identification of hucho taimen and an application thereof. The nucleotide sequence of the microsatellite marker is as shown in SEQ ID NO: 1. The method for identifying the hereditary sex of hucho taimen comprises the following steps: collecting fin ray samples of female and male hucho taimen; extracting genomic DNA of the fin ray sample, and carrying out PCR amplification; and carrying out electrophoresis detection on the obtained PCR amplification product; wherein when one strip of 208bp is displayed through electrophoresis, the Hucho taimen is female, and when strips of 208bp and 331bp2 are displayed, the Hucho taimen is male. The microsatellite marker and the identification method can accurately and efficiently identify the sex of the hucho taimen, fish killing is not needed, the method is simple and rapid, tedious sequencing steps are omitted, the identification accuracy is improved, and the microsatellite marker and the identification method can also be used for early sex identification of the hucho taimen.

Description

technical field [0001] The invention relates to the identification of the genetic sex of the salmon, in particular to a microsatellite marker, a primer and an application for accurately identifying the genetic sex of the salmon. Background technique [0002] Hucho taimen (pallas) belongs to the order Salmoniformes, the family Salmonidae, and the genus Hucho. It is a rare and valuable cold-water fish in my country, with tender meat and delicious taste. Zheluo salmon is the largest fish in the family Salmonidae. It grows rapidly and has no secondary sexual characteristics when it is sexually mature. In the wild, female fish are sexually mature at 5 to 6 years old, and male fish are sexually mature at 4 to 5 years old. Sexually mature Jero salmon have marriage color only during the breeding season, and the tail of the fish body is red, and the marriage color of some parent fish is not obvious. The marriage color of Jelo salmon in different waters is also different, and it is di...

AI Technical Summary

Problems solved by technology

Sexually mature Jero salmon have marriage color only during the breeding season, the tail of the fish body is red, and the marriage color of some parent fish is not obvious, and the marriage color of Jelo salmon in different waters is also different, so it is difficult to identify gender by marriage color

[0003] Zheluo salmon is a rare and precious cold-water fish with scarce resources. When identifying the sex of wild fish, it is necessary to kill the fish, take gonads, and paraffin sections to identify females and males. Not only is the process cumbersome, but it is also impossible to fully understand the sex ratio. Juvenile fish could not be identified

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