Hucho taimen sperm activation solution and preparation method thereof
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A sperm and liquid technology, applied in the preparation of salmon sperm activation solution, the field of salmon sperm activation solution
Inactive Publication Date: 2015-12-09
XINJIANG AGRI UNIV
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[0004] The invention provides a jelly salmon sperm activation solution and a preparation method. The invention solves how to reduce the energy consumed in osmotic pressure regulation, provide sufficient energy for the movement of sperm flagella, prolong the movement time of jelly salmon sperm, and greatly improve The chance of its sperm and egg combining, improving the fertilization rate
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Embodiment 1
[0020] A preparation method of jelly salmon sperm activation solution, the activation solution should be used as it is prepared, and the preparation method is as follows:
[0021] The activation solution is made of the following raw materials: glucose 40.64-81.27mmol / L, sodium chloride 10.12-20.24mmol / L, water temperature: 8-11°C, osmotic pressure: 142.41kpa-284.81kpa; the solvent for preparing the activation solution is deionized water or distilled water;
[0022] Solute mass calculation formula: solute mass = solute mass concentration x activation solution volume x solute molecular weight ÷ 1000;
[0023] Preparation steps:
[0024] (1) Calculate the glucose mass required for the volume of the activation solution according to the solute mass calculation formula, and use an electronic balance with an accuracy of 0.01g to accurately weigh the analytically pure glucose of this mass and put it into a beaker;
[0025] (2) Calculate the mass of glucose required for the volume of...
Embodiment 2
[0028] 1. Broodstock selection
[0029] There are 3 parent fishes selected in this experiment, with an average body weight of 5.5kg and an age of 6. + above. The broodstock is healthy, without trauma, and the gonads are mature, and the semen can flow out automatically when the abdomen is lightly pressed.
[0030] 2. Preparation of activation solution
[0031] 2.1. Raw material of activation solution
[0032] Analytical pure glucose, analytical pure sodium chloride, distilled water. The molecular weight of glucose is 180.16g / mol; the molecular weight of sodium chloride is 58.44g / mol.
[0033] According to calculation formula (calculation formula of parts by weight: parts by weight=solute mass concentration X activation solution volume (L) X solute molecular weight ÷ 1000), calculate the weight of glucose and sodium chloride required for preparing 50ml activation solution, glucose is 0.61g , sodium chloride is 0.05g, using an electronic balance to accurately weigh the two r...
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Abstract
The invention provides a Hucho taimen sperm activation solution and a preparation method thereof, and relates to the technical field of sperms of Hucho taimen species. The Hucho taimen sperm activation solution comprises the following raw materials: 40.64-81.27 mmol / L of glucose and 10.12-20.24 mmol / L of sodium chloride, wherein water temperature is 8-11 DEG C and osmotic pressure is 142.41 kPa to 284.81 kPa. The method comprises: weighing pure glucose and putting the pure glucose in a beaker; weighing analytically pure sodium chloride in the mass and putting the analytically pure sodium chloride in same beaker with the weighed glucose; adding deionized water or distilled water in half volume of the activation solution in the beaker, and stirring, transferring the dissolved solution in a volumetric flask whose capacity is the same with the prepared activation solution, pouring washed solution in the volumetric flask, and finally diluting with deionized water or distilled water to a scale mark, and shaking the volumetric flask. The activation solution and the preparation method solve problems of how to prolong movement time of Hucho taimen sperms and improve fertility rate.
Description
technical field [0001] The invention relates to the technical field of the sperm of the salmon species, in particular to a sperm activation solution of the salmon, and also to a preparation method of the sperm activation solution of the salmon. Background technique [0002] Huchotaimen, also known as big red fish, belongs to the order Salmonformes, Salmonidae, and the genus Huchotaimen. It is a famous large-scale ferocious carnivorous cold-water freshwater fish in my country. value. Due to the destruction of the living environment and a large number of overfishing, there are only a small number of them distributed in Kanas Lake in Xinjiang, the upper reaches of the Ussuri River and the Huma River in Heilongjiang in China. They are now national second-class protected animals and listed in the "China Red Book of Endangered Animals". [0003] In order to protect the germplasm resources of jerky salmon, it is necessary to carry out artificial domestication and breeding of jerky ...
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