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Method for obtaining high-quality Hucho taimen sperms through screening

A technology of Menjero salmon and Zheluo salmon, which is applied in the field of screening high-quality sperm of Zheluo salmon, can solve the problems of high deformity rate, low fertilization rate and hatching rate, and inability to determine the quality of sperm, so as to achieve a simple method and improve breeding efficiency , The effect of saving feeding costs

Active Publication Date: 2015-03-25
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims to solve the problems that the sperm quality cannot be determined at present, resulting in low fertilization rate, hatching rate and high deformity rate in the actual production process, and provides a method for screening high-quality sperm of Taimen jellyfish

Method used

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Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0013] Specific implementation mode 1: In this implementation mode, the high-quality sperm of Taimenzhe salmon are screened according to the following steps:

[0014] 1. Measure the content of Se and Ze in the testosterone in the serum of Taimenzhe salmon and in the semen;

[0015] 2. Select the semen of Taimen Zhe salmon whose serum is 4.9175μg / mg0.1024μg / mg and Ze>11.8725μg / mg, that is, complete Taimen Zhe Screening of high-quality spermatozoa from Taimenzhe salmon.

specific Embodiment approach 2

[0016] Specific embodiment two: the difference between this embodiment and specific embodiment one is: step one measures the concentration of trace element Se and Ze in the seminal fluid and carries out according to the following steps:

[0017] 1. Shake and mix the semen sample, then take 0.5ml into the microwave digestion tank, and then add 2.5ml HNO 3 and 2.5ml H 2 o 2 Carry out microwave digestion, the microwave digestion temperature rises from room temperature to 180°C within 15 minutes, then heat-preserve and digest at 180°C for 10 minutes, filter after digestion, and clear the solution to constant volume for testing;

[0018] 2. Using the standard curve method, use 1% HNO 3 Dilute the multi-element standard solution to make a series of standard working solutions with a concentration of 0, 10, 20, 50, 100 and 200 μg / L, and use the American Agilent7500 inductively coupled plasma mass spectrometer for measurement. Each fish semen sample is measured in 3 parallel . Othe...

specific Embodiment approach 3

[0019] Specific embodiment three: the difference between this embodiment and specific embodiment one is: the concentration of testosterone in step one measurement serum is carried out according to the following steps:

[0020] Collect 5ml of serum from each male fish, put it in a centrifuge at 3500r / min for 15min, take it out and measure the concentration of serum testosterone with Beckman Access Ⅱ chemiluminescence immunoassay analyzer and detection kit, and measure 3 serum samples of each male fish parallel. Other steps and parameters are the same as those in Embodiment 1.

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Abstract

The invention relates to a method for obtaining high-quality Hucho taimen sperms through screening. The problems that the fertilization rate and the hatching rate are low, and the malformation rate is high in the actual production process due to the fact that the sperm quality cannot be determined in the prior art are solved. The method comprises the steps that (1) the content of Se and Ze in seminal fluid and testosterone in serums of Hucho taimen is measured; (2) seminal fluid of the male Hucho taimen with serums with testosterone being larger than 4.9175 microgram / mg and smaller than 12.4951 microgram / mg is selected, wherein the content of Se in the seminal fluid is larger than 0.1024 microgram / mg and the content of Ze in the seminal fluid is larger than 11.8725 microgram / mg; the high-quality Hucho taimen sperms are obtained. The high-quality Hucho taimen sperms obtained through screening according to the method are high in fertilization rate and hatching rate, low in malformation rate, the artificial Hucho taimen feeding efficiency is greatly improved, and the feeding cost is saved.

Description

technical field [0001] The invention relates to a method for screening high-quality sperm of jelly salmon. Background technique [0002] Salmonisdae and Hucho belong to the family Salmonisdae. Taimenzhe salmon are mainly distributed in Russia, China, Mongolia and Japan. In history, Zheluo salmon were widely distributed in my country. In 2003, archaeologists found pictures of people capturing Zheluo salmon from the murals of the ancient city of Loulan buried in the desert. At present, wild salmon is an endangered species in my country, and only a small number of breeding populations exist in Wusuli River, Heilongjiang River and Hanas Lake in Xinjiang. As a typical representative of my country's indigenous cold-water fish, Taimenzhe salmon has extremely important scientific research significance and social value in terms of germplasm resource protection and breeding benefits. At the same time, Taimeng salmon is also a tourist fishing species with an international brand, whi...

Claims

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Application Information

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IPC IPC(8): A01K61/00G01N27/62
CPCA01K61/00G01N21/76G01N27/62G01N33/53Y02A40/81
Inventor 张永泉尹家胜张颖佟广香王海涛
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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