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Preparation method of Hucho taimen triploid fry

A technology of triploid and jelly salmon, which is applied in the field of preparation of jerky salmon triploid seedlings, to achieve the effects of low cost, improved disease resistance, and good market value

Inactive Publication Date: 2019-11-19
北京市水生野生动植物救护中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technique of triploid induction in Derma salmon has not been reported at home and abroad

Method used

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Embodiment 1

[0036] A preparation method for triploid salmon seedlings, specifically comprising the steps of:

[0037] 1. Breeding of broodstock: select 6 + Above, strong physique, well-developed broodstock artificially bred, the size of male broodstock is over 3000g, and the size of female broodstock is over 3500g. The ratio of male to female fish is 1:1. The feed is a special compound feed for salmon and trout broodstock, with a protein content of more than 40%, and fresh bait is fed regularly. 1 month before delivery to strengthen cultivation.

[0038] 2. Obtaining diploid fertilized eggs: the minimum water temperature for oxytocin is 6°C, and the oxytocin agent is LHRH-A produced by Ningbo Hormone General Factory 2 and DOM. LHRH-A per kilogram of fish in female fish 1 month before birth 2 1ug ripening. One week before birth, the dose to induce labor is 5ug LHRH-A per kilogram of fish for females 2 +5mg DOM; male fish halved. The effect period is 7-10 days. Broodstock were anes...

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Abstract

The invention provides a preparation method of a Hucho taimen triploid fry. The preparation method comprises: performing triploid heat shock induction, specifically, 15 min after fertilizing a diploidoosperm, putting the oosperm into a water bath at 17 DEG C for transition for 1 min, then putting the oosperm into a water bath at 26-27 DEG C for heat treatment for 15 min and then putting the oosperm into a water bath at 17 DEG C for transition for 1 min; incubating the oosperm, i.e., putting the oosperm subjected to triploid heat shock induction into a container at an incubation water temperature of 7-9 DEG C for incubation. By adopting the method, after 30- 40 days, the Hucho taimen triploid fry can be obtained; during incubation, the eyed rate of the oosperm is 80% or more, the incubation rate is 70% or more, and the triploid rate is up to 90% or more. The method provided by the invention effectively alleviates the problems of reduced growth rate after sexual maturity, reduced fullness, high mortality after broodstock reproduction and the like, improves disease resistance, and is suitable for producing large-sized fish for preparing sashimi, has the advantages of low cost, easiness in operation and the like, and has good market value.

Description

technical field [0001] The invention relates to the technical field of jellyfish breeding, in particular to a method for preparing jellyfish triploid seedlings. Background technique [0002] Hucho taimen, also known as Hucho taimen, belongs to Salmoniformes, Salmonidae, and Hucho taimen. It is a rare and endangered cold-water fish in the world. It is mainly distributed in northern Asia and in the northeast of China. The waters of Heilongjiang, Yalu River, Tumen River and Irtysh River in Xinjiang are currently extremely rare in the wild and have important protection and economic value. The broodstock of Jerome salmon has the habit of burying eggs and protecting the nest. After spawning, the broodstock dies in large numbers, especially the male fish. Due to the ferocious temperament and the indistinct sex characteristics of male and female, it is difficult to master the artificial reproduction technology. Breeding large-scale triploid salmon can increase the ratio of fish to...

Claims

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Application Information

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IPC IPC(8): A01K67/027A01K61/10A01K61/17
CPCA01K67/0275A01K2227/40A01K61/10A01K61/17Y02A40/81
Inventor 魏凯陈春山马龙耿琰郑伟
Owner 北京市水生野生动植物救护中心
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