In vitro culture method for hucho taimen body cells

A culture method and cell culture technology are applied in the field of in vitro culturing of salmonella somatic cells, which can solve the problem that cell culture research has not yet been reported, and achieve the effects of good cell growth, increasing the number of cell divisions, and promoting fishery health.

Inactive Publication Date: 2012-07-25
HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, although scholars at home and abroad have done a lot of research on the ecological habits, growth and reproduction, biochemistry and genetics of the salmon, the research on its cell culture has not yet been reported.

Method used

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  • In vitro culture method for hucho taimen body cells
  • In vitro culture method for hucho taimen body cells
  • In vitro culture method for hucho taimen body cells

Examples

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specific Embodiment approach 1

[0008] Specific embodiment one: the method for in vitro culture of salmon somatic cells in this embodiment is carried out according to the following steps: one, the neck of the salmon is executed; two, the body tissue of the salmon is collected, and the tissue is put into a solution containing 100IU / mL penicillin and Soak in 100IU / mL streptomycin in D-Hanks buffer for 20 seconds, then rinse with D-Hanks buffer 3 times to obtain the cleaned tissue; 3. Primary cultured tissue block: cut the cleaned tissue into pieces 0.8~1.2mm 3 block, then move the shredded tissue block into the cell culture bottle, spread the tissue block evenly on the bottom of the cell culture bottle, add 0.5mL cell culture solution to the cell culture bottle, and place the cell culture bottle upside down at 19°C After culturing for 4 hours, turn the cell culture flask over, add 8 mL of cell culture solution to the cell culture flask, and continue culturing in an incubator at 19°C until the tissue block cove...

specific Embodiment approach 2

[0009] Embodiment 2: The difference between this embodiment and Embodiment 1 is that in step 1, the specific method of killing the Jerome by cutting off the neck is as follows: select a Jerome with a body length of 12-14 cm, and insert it into the Jerome with medical pointed tweezers. In the mouth of the salmon, the other hand holds the body of the fish, and the neck is cut off by dorsal displacement. Others are the same as in the first embodiment.

specific Embodiment approach 3

[0010] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that: the body tissue of the salmon in step 2 is liver, kidney, fin edge or snout. Others are the same as in the first or second embodiment.

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Abstract

The invention relates to and provides an in vitro culture method for hucho taimen body cells. The in vitro culture method for the hucho taimen body cells comprises the following steps of: 1, killing a hucho taimen by braking the neck; 2, collecting hucho taimen body tissues; 3, performing primary culture on tissue blocks; 4, performing primary culture on cells; and 5, performing secondary cultureon the body cells, thus finishing the in vitro culture for the hucho taimen body cells. The cells cultured by the method have good cell growing state. The cell division times can be effectively increased by the method, so that the practical division times of the hucho taimen body cells are up to over 30.

Description

technical field [0001] The invention relates to a method for in vitro culturing of somatic cells of jellyfish. Background technique [0002] Hucho taimen is one of the rare and cold-water fishes in my country. It is mainly distributed in northern Asia, from the Volga River Basin in the west, the Pechora River Basin in the east, the Heilongjiang River Basin in the south, and the Lena River in the north. watershed. Jero salmon live in fast-flowing streams most of the time, overwinter in deeper water bodies such as main streams of major rivers and lakes in winter, and migrate to streams to lay eggs in spring. Due to overfishing during the breeding season, the number of supplementary populations of jelly salmon has been greatly reduced, as well as man-made environmental pollution and the destruction of living environment conditions, etc., the natural resources of jelly salmon have dropped significantly. At present, the number of jelly salmon in Heilongjiang has decreased signif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071
Inventor 卢彤岩郭振华赵吉伟王荻李绍戊刘红柏
Owner HEILONGJIANG RIVER FISHERY RES INST CHINESE ACADEMY OF FISHERIES SCI
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