Hydroponic method for promoting growth of radix ranunculi ternati roots
By adding bergamot fruit enzyme, glycine and iron sulfate complex, and a mixture of polyoxyethylene lauryl ether and saponin into the hydroponic solution, the permeability and uniformity of the nutrient solution are improved, solving the problems of slow growth of cat's claw root tubers and low nutrient absorption efficiency, and achieving rapid growth and healthy development of the root tubers.
Patent Information
- Application Number
- CN202510822402.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-19
- Publication Date
- 2025-09-09
AI Technical Summary
In existing hydroponic methods, the root tubers of cat's claw grow slowly and the efficiency of nutrient absorption is low, which makes it difficult to meet its needs at different growth stages.
A complex of bergamot fruit enzyme, glycine and ferric sulfate is used with a mixture of polyoxyethylene lauryl ether and saponin to form stable complexes and mixed micelles, thereby improving the permeability and uniformity of the nutrient solution, and increasing the concentration of effective ingredients in the nutrient solution through activation and fermentation of yeast powder.
Significantly improve the biomass accumulation and nutrient absorption efficiency of cat's claw root tubers, promote the growth and development of root tubers, reduce root oxidative damage, and increase the healthy growth rate of plants.
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Figure CN120604730A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of plant cultivation, and in particular to a hydroponic method for promoting the growth of cat's claw root tubers. Background Art
[0002] Cat's claw, also known as small buttercup and three-scattered grass, is an annual herbaceous plant in the genus Ranunculaceae. Its spindle-shaped rhizomes, often clustered in 5-6 pieces, resemble a cat's claw, hence the name. It is primarily found in central, southern, and eastern my country, growing in wet meadows, field edges, and hillside grasses. Its rhizomes have numerous medicinal properties, including expectorant, detoxification, swelling, antibacterial, anti-inflammatory, analgesic, and immune-boosting, and are valued in traditional medicine.
[0003] There are two methods for cultivating cat's claw: soil culture and hydroponics. According to the conventional soil culture method, the rhizomes of cat's claw begin to germinate seedlings in early October, and the seeds emerge from early October to March of the following year. December to April of the following year is the rapid growth period of cat's claw plants. Soil-cultured plants have the advantages of rich nutrition, strong water and fertilizer retention capacity, high stability, suitability for a variety of plants and low cost, but there are also disadvantages such as high risk of pests and diseases, unbalanced nutrients, soil compaction, complex management and large environmental impact. Therefore, hydroponics has become a new method for cultivating plants, especially plant roots and rhizomes. Hydroponic plants do not require soil, avoiding possible pathogens, insect eggs and other harmful organisms in the soil, reducing the chance of pests and diseases, and reducing the risk of plant illness. Moreover, the nutrient solution used for hydroponic plants is scientifically proportioned and can provide plants with comprehensive and balanced nutrients to meet the needs of plants at different growth stages, which is conducive to the healthy growth and development of plants.
[0004] Under suitable hydroponic conditions, the growth rate of cat's claw root tubers is relatively slow, but can gradually increase. The specific growth amount is affected by factors such as environmental conditions and the initial root tuber size. Hydroponics has high requirements for water quality and requires regular water replacement and nutrient solution addition, otherwise it is easy to cause root rot. Existing hydroponic solutions generally add nutrients, plant growth regulators and other ingredients, and require careful management to ensure the growth rate of cat's claw. This is because cat's claw is originally a native plant, and conventional nutrient solutions can only provide nutrition and cannot promote cat's claw to absorb nutrients in the water. Therefore, the present invention provides a hydroponic method for promoting the growth of cat's claw root tubers, improving the biomass accumulation and nutrient absorption efficiency of cat's claw root tubers, thereby solving the problems existing in the prior art. Summary of the Invention
[0005] In view of the shortcomings of the prior art, the object of the present invention is to provide a hydroponic method for promoting the growth of cat's claw rhizomes.
[0006] A hydroponic method for promoting the growth of cat's claw root tubers comprises the following steps: S1: Preparation of cat's claw hydroponic solution Weigh dry yeast powder and add it to a glucose solution, then add calcium glycinate and maltodextrin to obtain activated yeast powder, slice fresh bergamot fruit into thin slices, dry the slices to obtain bergamot fruit dry powder, weigh bergamot fruit dry powder, add distilled water, add pectinase, let stand, then add glucose solution, heat and stir, sterilize, let stand and cool, inoculate activated yeast powder and ferment to obtain bergamot fruit enzyme liquid, weigh bergamot fruit enzyme liquid and ferric sulfate solution, then add glycine, stir, centrifuge and concentrate, freeze-dry to obtain enzyme chelated iron, mix purified water, calcium nitrate, potassium nitrate and magnesium nitrate to obtain a nutrient solution, add enzyme chelated iron to the nutrient solution, then add a mixture of polyoxyethylene lauryl ether and saponin, and stir evenly to obtain a cat's claw hydroponic solution; S2: Hydroponics of cat's claw root tubers After the cat's claw rhizomes are propagated into seedlings, they are moved into hydroponic pots for cultivation. Add cat's claw hydroponic solution into the hydroponic pots and cultivate at room temperature.
[0007] Furthermore, step S1 of preparing a cat's claw hydroponic solution comprises the following steps: S1.1: Weigh 5-10 parts by weight of dry yeast powder and add it to 50-60 parts by weight of glucose solution. Then add 5-8 parts by weight of calcium glycinate and 10-15 parts by weight of maltodextrin. Oscillate on a constant temperature shaker at 28-30°C for 3-5 hours to obtain activated yeast powder. S1.2: Cut fresh bergamot into thin slices, place in a constant temperature drying oven at 60-65℃ and dry for 50-55h, crush and pass through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 5-6 parts by mass of bergamot dry powder, add 20-22 parts by mass of distilled water, then add 0.2-0.4 parts by mass of pectinase, let stand for 15-20min, then add 10-15 parts by mass of glucose solution, heat to 100-105℃ at a speed of 4-5℃ / min, and stir at a speed of 50-60r / min until the required temperature is reached, then place Sterilize in an autoclave at 115-120°C for 30-35 minutes, then cool to room temperature of 25-28°C, inoculate with activated yeast powder, and ferment in a constant temperature box for 40-48 hours to obtain bergamot fruit enzyme liquid. Weigh 15-20 parts by weight of bergamot fruit enzyme liquid and 5-8 parts by weight of ferric sulfate solution, then add 4-6 parts by weight of glycine, stir at room temperature of 25-28°C for 30-35 minutes, then centrifuge and concentrate at a speed of 3000-3400 r / min for 10-15 minutes, and then freeze-dry for 5-6 hours to obtain enzyme chelated iron; S1.3: Mix purified water, calcium nitrate, potassium nitrate and magnesium nitrate in a mass ratio of 1000: (0.1-0.2): (0.1-0.2): (0.1-0.2) to prepare a nutrient solution, then add 3-5% of the system weight of enzyme-chelated iron to the nutrient solution, stir for 10-20 minutes, and then add 5-10% of the system weight of a mixture of polyoxyethylene lauryl ether and saponin, stir evenly to obtain a cat's claw hydroponic solution.
[0008] Furthermore, step S2 of hydroponically cultivating cat's claw root pieces comprises the following steps: After the cat's claw root tubers are propagated into seedlings, they are moved into hydroponic pots for cultivation. Cat's claw hydroponic solution is added to the hydroponic pot, and the depth of the cat's claw hydroponic solution is set to 3-5 cm. The root tubers of the cat's claw seedlings are wrapped with sponge blocks and placed in the hydroponic pot for cultivation at room temperature of 18-22°C. The cat's claw hydroponic solution is controlled to circulate in the pot and the depth of the cat's claw hydroponic solution is maintained. The light intensity is 2800-3000 Lux, and the lighting time is 9:00-21:00 every day.
[0009] Furthermore, the mass fraction of the glucose solution is 2-3%.
[0010] Furthermore, the concentration of the ferric sulfate solution is 0.05-0.1 mol / L.
[0011] Furthermore, the thickness of the slices of fresh bergamot fruit is 5-8 mm.
[0012] Furthermore, the polyoxyethylene lauryl ether and saponin mixed solution is specifically prepared by mixing polyoxyethylene lauryl ether and saponin at a mass ratio of 1: (1-1.5) and then centrifuging at a high speed of 8000-9000 r / min for 5-10 minutes.
[0013] Compared with the prior art, the present invention has at least the following beneficial effects: 1. The present invention complexes the iron ions in bergamot fruit enzyme, glycine and ferric sulfate. Glycine, as a small molecule organic ligand, can form a stable soluble complex with iron ions, avoid the precipitation of iron ions in the hydroponic solution, and form a complex such as glycine iron. At the same time, the organic matter degraded by the enzyme forms an organic-inorganic composite nutrition system with the glycine iron. Moreover, the antioxidant components of the enzyme can reduce root oxidative damage and balance oxidative stress when iron is excessive, thereby improving the absorption efficiency of glycine iron by the plant root system and reducing energy consumption. This combination significantly improves the biomass accumulation and nutrient absorption efficiency of cat's claw root tubers through organic-inorganic synergy, enzymatic activation and hormone regulation, thereby achieving the effect of promoting the growth of cat's claw root tubers.
[0014] 2. The present invention adds polyoxyethylene lauryl ether and saponin to the hydroponic solution. When mixed with saponin, mixed micelles can be formed, which significantly reduces the critical micelle concentration and increases the micelle-water partition coefficient, thereby improving the permeability of the cat's claw hydroponic solution. When the cat's claw hydroponic solution is circulated in the hydroponic basin, the improved permeability helps the nutrients in the cat's claw hydroponic solution to be evenly distributed around the plant roots, so that the plant roots can more effectively absorb the nutrients in the hydroponic solution. In addition, the affinity between the surface of the cat's claw root block and the nutrients can be adjusted, and the absorption of nutrients by the cat's claw root block can be promoted, thereby promoting the growth and development of the cat's claw root block.
[0015] 3. The present invention activates dry yeast with a mixture of glucose solution, calcium glycinate and maltodextrin. Calcium glycinate can form weak coordination bonds with groups in maltodextrin molecules to prevent excessive aggregation of polysaccharide chains. At the same time, calcium glycinate can also chelate with glucose molecules. Maltodextrin and glucose are complexed by calcium glycinate, and the uniformity and stability of the two in the activation system are improved at the same time, so that the maltodextrin molecules and glucose molecules are evenly distributed in the system to activate the yeast powder. In addition, maltodextrin, as a source of easily digestible carbohydrates, can provide continuous energy for yeast and help it to reproduce rapidly. Glucose is the most direct energy source for yeast and can be quickly metabolized, accelerating the initial activation of yeast powder, thereby improving the activation effect of yeast powder throughout the entire period, improving fermentation efficiency, and thereby increasing the yield of bergamot enzyme, so that the bergamot enzyme in the prepared cat's claw hydroponic solution has higher purity and a higher content proportion, thereby improving the promotion effect on the growth of cat's claw root tubers. BRIEF DESCRIPTION OF THE DRAWINGS
[0016] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the present disclosure and, together with the description, further serve to explain the principles of the present disclosure and to enable one skilled in the relevant art to make and use the present disclosure.
[0017] Figure 1 This is a photo of the hydroponic culture of cat's claw according to an embodiment of the present invention.
[0018] Figure 2 This is a photo of a root piece of cat's claw according to an embodiment of the present invention.
[0019] Figure 3 This is a photo of the whole plant of Cat's Claw according to an embodiment of the present invention. DETAILED DESCRIPTION
[0020] The following is a detailed description of a hydroponic method for promoting the growth of cat's claw root tubers provided by the present invention, in conjunction with the accompanying drawings and specific embodiments. It is also noted that, in order to make the embodiments more detailed, the following embodiments are best and preferred embodiments, and those skilled in the art may also adopt other alternative methods for implementation of some known technologies; and the accompanying drawings are only for more specific descriptions of the embodiments, and are not intended to specifically limit the present invention.
[0021] Example 1
[0022] A hydroponic method for promoting the growth of cat's claw root tubers comprises the following steps: S1: Preparation of cat's claw hydroponic solution S1.1: Weigh 5 parts by mass of dry yeast powder and add it to 50 parts by mass of 2% glucose solution. Then add 5 parts by mass of calcium glycinate and 10 parts by mass of maltodextrin. Oscillate on a constant temperature shaker at 28°C for 3 hours to obtain activated yeast powder. S1.2: Cut fresh bergamot into thin slices with a thickness of 5 mm, place in a constant temperature drying oven at 60°C and dry for 50 h, grind and pass through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 5 parts by mass of bergamot dry powder, add 20 parts by mass of distilled water, then add 0.2 parts by mass of pectinase, let it stand for 15 minutes, then add 10 parts by mass of 2% glucose solution, heat to 100°C at a speed of 4°C / min, and stir at a speed of 50 r / min until the required temperature is reached. The mixture was placed in an autoclave at 115°C for 30 minutes, and then allowed to stand and cool to room temperature of 25°C. Activated yeast powder was inoculated and fermented in a constant temperature box for 40 hours to obtain a bergamot fruit enzyme solution. 15 parts by mass of the bergamot fruit enzyme solution and 5 parts by mass of a 0.05 mol / L ferric sulfate solution were weighed, and 4 parts by mass of glycine were added. The mixture was stirred at room temperature of 25°C for 30 minutes, and then centrifuged and concentrated at a speed of 3000 r / min for 10 minutes, and then freeze-dried for 5 hours to obtain enzyme chelated iron. S1.3: Mix pure water, calcium nitrate, potassium nitrate and magnesium nitrate in a mass ratio of 1000:0.1:0.1:0.1 to prepare a nutrient solution, then add 3% of the system mass of enzyme chelated iron to the nutrient solution, stir for 10 minutes, and then add 5% of the system mass of a mixed solution of polyoxyethylene lauryl ether and saponin, stir evenly to obtain a cat's claw hydroponic solution; wherein the mixed solution of polyoxyethylene lauryl ether and saponin is specifically prepared by mixing polyoxyethylene lauryl ether and saponin in a mass ratio of 1:1 and then high-speed centrifuging at 8000r / min for 5 minutes.
[0023] S2: Hydroponics of cat's claw root tubers After the cat's claw root tubers are propagated into seedlings, they are moved into hydroponic pots for cultivation. Cat's claw hydroponic solution is added to the hydroponic pot, and the depth of the cat's claw hydroponic solution is set to 3 cm. The root tubers of the cat's claw seedlings are wrapped with sponge blocks and placed in the hydroponic pot for cultivation at room temperature of 18°C. The cat's claw hydroponic solution is controlled to circulate in the pot and the depth of the cat's claw hydroponic solution is maintained. The light intensity is 2800 Lux, and the lighting time is 9:00-21:00 every day. Figure 1 This is a photo of the cat's claw hydroponic culture. Figure 2 Here is a photo of cat's claw root block, Figure 3 This is a photo of the whole plant of cat's claw.
[0024] Example 2
[0025] A hydroponic method for promoting the growth of cat's claw root tubers comprises the following steps: S1: Preparation of cat's claw hydroponic solution S1.1: Weigh 10 parts by mass of dry yeast powder and add it to 60 parts by mass of 3% glucose solution. Then, add 8 parts by mass of calcium glycinate and 15 parts by mass of maltodextrin. Oscillate on a constant temperature shaker at 28°C for 3 hours to obtain activated yeast powder. S1.2: Cut fresh bergamot into thin slices with a thickness of 5 mm, place in a constant temperature drying oven at 60°C and dry for 50 h, grind and pass through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 6 parts by mass of bergamot dry powder, add 22 parts by mass of distilled water, then add 0.4 parts by mass of pectinase, let it stand for 15 minutes, then add 15 parts by mass of 3% glucose solution, heat to 100°C at a speed of 4°C / min, and stir at a speed of 50 r / min until the required temperature is reached. The mixture was placed in an autoclave at 115°C for 30 minutes, and then allowed to stand and cool to room temperature of 25°C. Activated yeast powder was inoculated and fermented in a constant temperature box for 40 hours to obtain a bergamot fruit enzyme solution. 20 parts by mass of the bergamot fruit enzyme solution and 8 parts by mass of a 0.05 mol / L ferric sulfate solution were weighed, and 6 parts by mass of glycine were added. The mixture was stirred at room temperature of 25°C for 30 minutes, and then centrifuged and concentrated at a speed of 3000 r / min for 10 minutes, and then freeze-dried for 5 hours to obtain enzyme chelated iron. S1.3: Mix pure water, calcium nitrate, potassium nitrate and magnesium nitrate in a mass ratio of 1000:0.2:0.2:0.2 to prepare a nutrient solution, then add 5% of the system mass of enzyme chelated iron to the nutrient solution, stir for 10 minutes, and then add 10% of the system mass of a mixed solution of polyoxyethylene lauryl ether and saponin, stir evenly to obtain a cat's claw hydroponic solution; wherein the mixed solution of polyoxyethylene lauryl ether and saponin is specifically prepared by mixing polyoxyethylene lauryl ether and saponin in a mass ratio of 1:1 and then high-speed centrifuging at 8000r / min for 5 minutes.
[0026] S2: Hydroponics of cat's claw root tubers After the cat's claw root tubers are propagated into seedlings, they are moved into hydroponic pots for cultivation. Cat's claw hydroponic solution is added to the hydroponic pot, and the depth of the cat's claw hydroponic solution is set to 3 cm. The root tubers of the cat's claw seedlings are wrapped with sponge blocks and placed in the hydroponic pot for cultivation at room temperature of 18°C. The cat's claw hydroponic solution is controlled to circulate in the pot and the depth of the cat's claw hydroponic solution is maintained. The light intensity is 2800 Lux, and the lighting time is 9:00-21:00 every day.
[0027] Example 3
[0028] A hydroponic method for promoting the growth of cat's claw root tubers comprises the following steps: S1: Preparation of cat's claw hydroponic solution S1.1: Weigh 5 parts by mass of dry yeast powder and add it to 50 parts by mass of 2% glucose solution. Then add 5 parts by mass of calcium glycinate and 10 parts by mass of maltodextrin. Oscillate on a constant temperature shaker at 30°C for 5 hours to obtain activated yeast powder. S1.2: Cut fresh bergamot into slices with a thickness of 8 mm, place in a constant temperature drying oven at 65°C and dry for 55 h, grind and pass through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 5 parts by mass of bergamot dry powder, add 20 parts by mass of distilled water, then add 0.2 parts by mass of pectinase, let it stand for 20 min, then add 10 parts by mass of 2% glucose solution, heat to 105°C at a rate of 5°C / min, and stir at a speed of 60 r / min until the required temperature is reached. The mixture was placed in an autoclave at 120°C for 35 minutes, and then allowed to stand and cool to room temperature of 28°C. Activated yeast powder was inoculated and fermented in a constant temperature box for 48 hours to obtain a bergamot fruit enzyme solution. 15 parts by mass of the bergamot fruit enzyme solution and 5 parts by mass of a 0.05 mol / L ferric sulfate solution were weighed, and 4 parts by mass of glycine were added. The mixture was stirred at room temperature of 28°C for 35 minutes, and then centrifuged and concentrated at a speed of 3400 r / min for 15 minutes, and then freeze-dried for 6 hours to obtain enzyme chelated iron. S1.3: Pure water, calcium nitrate, potassium nitrate and magnesium nitrate are mixed in a mass ratio of 1000:0.1:0.1:0.1 to prepare a nutrient solution, and then 3% of the system weight of enzyme chelated iron is added to the nutrient solution, stirred for 20 minutes, and then 5% of the system weight of polyoxyethylene lauryl ether and saponin mixed solution is added, and stirred evenly to obtain a cat's claw hydroponic solution; wherein the polyoxyethylene lauryl ether and saponin mixed solution is specifically prepared by mixing polyoxyethylene lauryl ether and saponin in a mass ratio of 1:1 and then centrifuging at a high speed of 9000r / min for 10 minutes. S2: Hydroponics of cat's claw root tubers After the cat's claw root tubers are propagated into seedlings, they are moved into hydroponic pots for cultivation. Cat's claw hydroponic solution is added to the hydroponic pots, and the depth of the cat's claw hydroponic solution is set to 5 cm. The root tubers of the cat's claw seedlings are wrapped with sponge blocks and placed in the hydroponic pots for cultivation at room temperature of 22°C. The cat's claw hydroponic solution is controlled to circulate in the pot and the depth of the cat's claw hydroponic solution is maintained. The light intensity is 3000 Lux, and the lighting time is 9:00-21:00 every day.
[0029] Comparative Example 1: Comparative Example 1: Commercially available plant hydroponic nutrient solution was used, and 30 plants of cat's claw were cultured under the same conditions using the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root tubers before and after hydroponic cultivation were calculated, which was recorded as Comparative Example 1.
[0030] Comparative Example 2: Compared with Example 1, the difference of Comparative Example 2 is that calcium glycinate is not added in step S1.1, specifically "S1.1: weigh 5 parts by mass of dry yeast powder and add it to 50 parts by mass of 2% glucose solution, then add 10 parts by mass of maltodextrin, and shake on a constant temperature shaker at 28°C for 3 hours to obtain activated yeast powder", and the other steps remain unchanged. A cat's claw hydroponic solution is prepared, and 30 cat's claw plants are cultured under the same conditions according to the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root blocks before and after hydroponics are counted, which is recorded as Comparative Example 2.
[0031] Comparative Example 3: Compared with Example 1, the difference of Comparative Example 3 is that maltodextrin is not added in step S1.1, specifically "S1.1: weigh 5 parts by mass of dry yeast powder and add it to 50 parts by mass of 2% glucose solution, then add 5 parts by mass of calcium glycinate, and shake on a constant temperature shaker at 28°C for 3 hours to obtain activated yeast powder", and the other steps remain unchanged, and a cat's claw hydroponic solution is prepared, and 30 cat's claw plants are cultured under the same conditions according to the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root blocks before and after hydroponics are counted, and recorded as Comparative Example 3.
[0032] Comparative Example 4: Compared with Example 1, the difference of Comparative Example 4 is that no ferric sulfate solution is added in step S1.2, specifically "S1.2: cut the fresh bergamot into thin slices with a thickness of 5 mm, place them in a constant temperature drying oven at 60°C and dry them for 50 hours, grind them and pass them through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 5 parts by mass of bergamot dry powder, add 20 parts by mass of distilled water, add 0.2 parts by mass of pectinase, let it stand for 15 minutes, then add 10 parts by mass of 2% glucose solution, heat it to 100°C at a speed of 4°C / min, and stir it at a speed of 50 r / min until it reaches the required temperature, and then put it into a sterilizer for 115 ℃ sterilization for 30 minutes, then standing and cooling to room temperature 25℃, inoculating activated yeast powder, and fermenting in a constant temperature box for 40 hours to obtain bergamot fruit enzyme liquid, weighing 15 parts by mass of bergamot fruit enzyme liquid, and then adding 4 parts by mass of glycine, stirring at room temperature 25℃ for 30 minutes, and then centrifuging and concentrating at a speed of 3000r / min for 10 minutes, and then freeze-drying for 5 hours to obtain enzyme chelate". The enzyme chelate is used instead of enzyme chelate iron for the subsequent steps to prepare cat's claw hydroponic solution, and 30 cat's claw plants are cultured under the same conditions by the method of step S2. After culturing for 60 days, the average length and diameter of cat's claw root pieces before and after hydroculture are counted, which is recorded as comparative example 4.
[0033] Comparative Example 5: Compared with Example 1, the difference of Comparative Example 5 is that glycine is not added in step S1.2, specifically, “S1.2: cut the fresh bergamot fruit into thin slices with a thickness of 5 mm, place them in a constant temperature drying oven at 60°C and dry them for 50 hours, grind them and pass them through a 40-mesh sieve to obtain bergamot fruit dry powder, accurately weigh 5 parts by mass of bergamot fruit dry powder, add 20 parts by mass of distilled water, add 0.2 parts by mass of pectinase, let it stand for 15 minutes, then add 10 parts by mass of 2% glucose solution, heat it to 100°C at a speed of 4°C / min, and stir it at a speed of 50 r / min until it reaches the required temperature, and then put it into a sterilizer and sterilize it at 115°C for 30 minutes. in, then allowed to stand and cool to room temperature 25°C, inoculated with activated yeast powder, and fermented in a constant temperature box for 40 hours to obtain a bergamot fruit enzyme liquid, weighed 15 parts by mass of bergamot fruit enzyme liquid and 5 parts by mass of a 0.05 mol / L ferric sulfate solution, stirred at room temperature 25°C for 30 minutes, and then centrifuged and concentrated at a speed of 3000 r / min for 10 minutes, and then freeze-dried for 5 hours to obtain an enzyme mixture". The enzyme mixture was used instead of enzyme chelated iron for the subsequent steps to prepare a cat's claw hydroponic solution, and 30 cat's claw plants were cultured under the same conditions by the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root block before and after hydroponics were counted, which was recorded as comparative example 5.
[0034] Comparative Example 6: Compared with Example 1, the difference of Comparative Example 6 is that polyoxyethylene lauryl ether is not added in step S1.3, specifically "S1.3: pure water, calcium nitrate, potassium nitrate and magnesium nitrate are mixed in a mass ratio of 1000:0.1:0.1:0.1 to prepare a nutrient solution, and then 3% of the system mass of enzyme chelated iron is added to the nutrient solution, stirred for 10 minutes, and then 5% of the system mass of saponin is added, and stirred evenly to obtain a cat's claw hydroponic solution", and the remaining steps remain unchanged, and a cat's claw hydroponic solution is prepared, and 30 cat's claw plants are cultured under the same conditions by the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root blocks before and after hydroponics are counted, which is recorded as Comparative Example 6.
[0035] The cat's claw hydroponic solution prepared in Examples 1-3 was used to culture 30 cat's claw plants under the same conditions using the method of step S2. After 60 days of culture, the average length and diameter of the cat's claw root pieces before and after hydroponic culture were counted, and the data were compared with those of Comparative Examples 1-6, as shown in Table 1.
[0036] Table 1: Data statistics results Initial length (mm) Initial diameter (mm) Length after 60 days (mm) Diameter after 60 days (mm) Example 1 6.2 2.1 9.6 2.8 Example 2 6.3 2.1 9.8 2.8 Example 3 6.2 2.1 9.7 2.8 Comparative Example 1 6.2 2.1 8.6 2.6 Comparative Example 2 6.2 2.1 8.4 2.4 Comparative Example 3 6.3 2.1 8.5 2.5 Comparative Example 4 6.3 2.1 8.4 2.2 Comparative Example 5 6.2 2.1 8.2 2.2 Comparative Example 6 6.2 2.1 8.6 2.6 As can be seen from Table 1, the initial lengths of Examples 1-3 and Comparative Examples 1-6 are 6.2-6.3mm, and the initial diameters are both 2.1mm. After 60 days of hydroponic cultivation, the lengths of Examples 1-3 increase to 9.6-9.8mm, and the diameter increases to 2.8mm. Comparative Example 1 is a commercially available plant hydroponic nutrient solution. It can be seen that after cultivation with the commercially available hydroponic nutrient solution, the length and diameter of the cat's claw root piece also increase, with the average length increasing from 6.2mm to 8.6mm, and the average diameter increasing from 2.1mm to 2.6mm. The average length and diameter increase of Examples 1-3 are higher than those of Comparative Example 1. It can be seen that the cat's claw hydroponic solution prepared by the present invention has a better promoting effect on the growth of the cat's claw root piece. Comparative Examples 2-6 are the experimental results of the present invention in which some raw material components are removed. It can be seen that the raw material combination of the present invention has a synergistic effect, and can only play a better role when used together. A single component cannot achieve the excellent effect achieved by the embodiment.
[0037] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the present invention. Anyone skilled in the art may modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by one of ordinary skill in the art without departing from the spirit and technical principles disclosed herein are intended to be covered by the claims of the present invention.
Claims
1. A hydroponic method for promoting the growth of cat's claw root tubers, characterized in that: The steps include: S1: Preparation of cat's claw hydroponic solution Weigh dry yeast powder and add it to a glucose solution, then add calcium glycinate and maltodextrin to obtain activated yeast powder, slice fresh bergamot fruit into thin slices, dry the slices to obtain bergamot fruit dry powder, weigh bergamot fruit dry powder, add distilled water, add pectinase, let stand, then add glucose solution, heat and stir, sterilize, let stand and cool, inoculate activated yeast powder and ferment to obtain bergamot fruit enzyme liquid, weigh bergamot fruit enzyme liquid and ferric sulfate solution, then add glycine, stir, centrifuge and concentrate, freeze-dry to obtain enzyme chelated iron, mix purified water, calcium nitrate, potassium nitrate and magnesium nitrate to obtain a nutrient solution, add enzyme chelated iron to the nutrient solution, then add a mixture of polyoxyethylene lauryl ether and saponin, and stir evenly to obtain a cat's claw hydroponic solution; S2: Hydroponics of cat's claw root tubers After the cat's claw rhizomes are propagated into seedlings, they are moved into hydroponic pots for cultivation. Add cat's claw hydroponic solution into the hydroponic pots and cultivate at room temperature.
2. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 1, characterized in that: Step S1: preparing a cat's claw hydroponic solution, comprising the following steps: S1.1: Weigh 5-10 parts by weight of dry yeast powder and add it to 50-60 parts by weight of glucose solution. Then add 5-8 parts by weight of calcium glycinate and 10-15 parts by weight of maltodextrin. Oscillate on a constant temperature shaker at 28-30°C for 3-5 hours to obtain activated yeast powder. S1.2: Cut fresh bergamot into thin slices, place in a constant temperature drying oven at 60-65℃ and dry for 50-55h, crush and pass through a 40-mesh sieve to obtain bergamot dry powder, accurately weigh 5-6 parts by mass of bergamot dry powder, add 20-22 parts by mass of distilled water, then add 0.2-0.4 parts by mass of pectinase, let stand for 15-20min, then add 10-15 parts by mass of glucose solution, heat to 100-105℃ at a speed of 4-5℃ / min, and stir at a speed of 50-60r / min until the required temperature is reached, then place Sterilize in an autoclave at 115-120°C for 30-35 minutes, then cool to room temperature of 25-28°C, inoculate with activated yeast powder, and ferment in a constant temperature box for 40-48 hours to obtain bergamot fruit enzyme liquid. Weigh 15-20 parts by weight of bergamot fruit enzyme liquid and 5-8 parts by weight of ferric sulfate solution, then add 4-6 parts by weight of glycine, stir at room temperature of 25-28°C for 30-35 minutes, then centrifuge and concentrate at a speed of 3000-3400 r / min for 10-15 minutes, and then freeze-dry for 5-6 hours to obtain enzyme chelated iron; S1.3: Mix purified water, calcium nitrate, potassium nitrate and magnesium nitrate in a mass ratio of 1000: (0.1-0.2): (0.1-0.2): (0.1-0.2) to prepare a nutrient solution, then add 3-5% of the system weight of enzyme-chelated iron to the nutrient solution, stir for 10-20 minutes, and then add 5-10% of the system weight of a mixture of polyoxyethylene lauryl ether and saponin, stir evenly to obtain a cat's claw hydroponic solution.
3. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 2, characterized in that: Step S2 is hydroponically cultivating cat's claw root tubers, comprising the following steps: After the cat's claw root tubers are propagated into seedlings, they are moved into hydroponic pots for cultivation. Cat's claw hydroponic solution is added to the hydroponic pot, and the depth of the cat's claw hydroponic solution is set to 3-5 cm. The root tubers of the cat's claw seedlings are wrapped with sponge blocks and placed in the hydroponic pot for cultivation at room temperature of 18-22°C. The cat's claw hydroponic solution is controlled to circulate in the pot and the depth of the cat's claw hydroponic solution is maintained. The light intensity is 2800-3000 Lux, and the lighting time is 9:00-21:00 every day.
4. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 3, characterized in that: The mass fraction of glucose solution is 2-3%.
5. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 4, characterized in that: The concentration of the ferric sulfate solution is 0.05-0.1 mol / L.
6. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 5, characterized in that: The thickness of fresh bergamot slices is 5-8mm.
7. A hydroponic method for promoting the growth of cat's claw root tubers according to claim 6, characterized in that: The polyoxyethylene lauryl ether and saponin mixed solution is prepared by mixing polyoxyethylene lauryl ether and saponin at a mass ratio of 1: (1-1.5) and then centrifuging at a high speed of 8000-9000 r / min for 5-10 minutes.