Nutrient solution for promoting sympodial bamboos to root and preparation process thereof

Abstract

The invention belongs to the technical field of plant nutrient solutions. The invention provides a nutrient solution for promoting sympodial bamboos to root. The nutrient solution is prepared from the following components in parts by weight: 1 to 2 parts of major elements, 0.4 to 0.8 part of trace elements, 0.001 to 0.003 part of amino acid, 0.002 to 0.004 part of plant growth hormone, 0.2 to 0.5 part of saccharides, 0.1 to 0.2 part of vitamins, 0.0015 to 0.002 part of an osmotic regulator, 0.3 to 0.4 part of chitosan, 0.08 to 0.12 part of polyacrylamide, 0.001 to 0.003 part of a nano material and 10 to 30 parts of water. The nutrient solution improves the rooting efficiency and survival rate of sympodial bamboos through a composite system of nutrition supply, hormone regulation, structure support and permeation protection, and has good development and strong stress resistance and adaptability in growth height, leaf number, root length and the like. And the uniformity and the stability of the nutrient solution can be ensured by a segmented dissolution mode.

Description

Technical Field

[0001] This invention relates to the field of plant nutrient solution technology, and in particular to a nutrient solution that promotes root growth in clump-forming bamboo and its preparation process. Background Technology

[0002] Bamboo is an important forest resource, characterized by its strong regeneration ability, rapid growth, short maturation period, and high yield. It possesses the characteristic of being "permanently" usable after a single afforestation effort, earning it the reputation of an "industry that can't be ruined." Bamboo wood boasts excellent longitudinal strength, high elasticity, wear resistance, corrosion resistance, bending resistance, insect resistance, and flame retardancy. Compared to wood, it offers advantages such as higher hardness, greater stability, better gluing properties, and a more attractive appearance. Besides its traditional uses in building houses, making furniture, and handicrafts, bamboo is primarily used to produce bamboo-based engineered wood products and high-grade bamboo paper, with demand increasing daily.

[0003] Bamboo shoots, the young shoots of bamboo, are crisp, refreshing, and nutritious, rich in 18 kinds of amino acids and various essential trace elements. They are a natural health food and have been developed into a series of products such as fresh bamboo shoots, dried bamboo shoots, and canned bamboo shoots, becoming a major pillar product in the development and utilization of bamboo resources. Bamboo is also an evergreen species. Its crisscrossing underground rhizome system (spreading bamboo) or its well-developed rhizome system with fibrous roots (clump bamboo) has a strong function of soil and water conservation. Although the above-ground parts produce shoots every year, and even with the annual selective felling of older bamboo culms and harvesting of fresh shoots, it can still maintain a relatively stable forest structure. Therefore, bamboo forests have unparalleled advantages over other trees in terms of soil and water conservation, water source conservation, climate regulation, air purification, and environmental beautification. Therefore, the rapid propagation of superior bamboo species is of great significance for the development and utilization of valuable bamboo resources. The key to its efficient propagation and rapid forest formation lies in the rooting quality of the seedlings. A well-developed root system not only improves the survival rate of seedlings but also enhances the plant's resistance to environmental stress.

[0004] In addition, the extensive root system of clumping bamboo not only increases soil stability but also improves soil structure; it is highly adaptable to climate, resistant to drought, cold, heat, and salinity, and can grow in relatively harsh environmental conditions; its relatively stable root system can withstand strong winds and play a role in windbreak and sand fixation; at the same time, clumping bamboo can provide habitat for a variety of animals and is a major food source for animals that depend on bamboo (pandas).

[0005] Currently, rooting techniques for clump-forming bamboo mainly rely on traditional methods, such as treating cuttings with single plant growth hormones (e.g., naphthaleneacetic acid, indolebutyric acid) or cultivating them using basic mineral nutrient solutions. However, the study "Experimental Research on the Introduction and Cultivation of Lei Bamboo" (from World Bamboo and Rattan Newsletter, 2005, Issue 3) compared the effects of a single plant hormone (indolebutyric acid) and a compound regulator (ABT3 rooting powder) on transplanted Lei Bamboo mother bamboo, revealing the limitations of single hormone treatment: 1. Significant differences in rooting effect: Mother bamboo treated with ABT3 (containing multiple nutrients and plant growth regulators) showed a 29% increase in rooting and rhizome length compared to the single indolebutyric acid treatment, with increased taproot length and a significantly increased number of lateral roots. In contrast, the root system treated with single indolebutyric acid grew slowly and was weak, making it difficult to form a robust root network. 2. Stress resistance and transplant survival rate: The transplant survival rate of mother bamboo treated with ABT3 was 4% higher than that treated with indolebutyric acid, and the shoot emergence rate was increased by 52%. However, mother bamboo treated with a single hormone suffered from poor root development, resulting in low resistance to drought and nutrient absorption, leading to a high mortality rate after transplanting and poor overall resistance after maturity. 3. The Importance of Synergistic Nutritional Effects: Studies indicate that the advantage of ABT3 stems from its complex formula (containing trace elements, organic matter, and multiple hormones), which provides comprehensive nutritional support to the root system. In contrast, single hormones (such as indolebutyric acid) can only induce root primordia formation in the short term, but lack sustained nutrient supply, resulting in insufficient resistance of newly formed roots. In other words, although single plant growth hormone treatment can induce root primordia formation in bamboo plants in a short time, the lack of comprehensive nutritional support leads to weak new roots, poor resistance, and a high mortality rate after transplanting, demonstrating the limited effectiveness of single plant hormone use. The study, "Research on Sprouting Technology of Secondary Branches of Clump-forming Bamboo in a Sprouting Nursery" (from Forestry Science Research, Vol. 2, 2000), systematically verified the limitations of traditional mineral nutrient solutions in the rooting of bamboo plants through orthogonal experimental design, and revealed the necessity of compound nutrient regulation and osmotic pressure regulation: 1. Deficiencies in the composition of traditional nutrient solutions: The study found that the sprouting rate of secondary branches of clump-forming bamboo relying solely on basic mineral nutrient solutions (such as those containing only nitrogen, phosphorus, and potassium) was significantly lower than that of treatments with added trace elements and physiologically active substances. For example, after adding MS nutrient solution containing 2 mg / L 6-BA + 1 mg / L NAA, the annual sprouting rate of secondary branches of green bamboo reached 142,500 branches / hectare, while the sprouting rate of the control group (basic mineral nutrient solution only) was less than 60% of that. 2. Synergistic effect of trace elements and amino acids: The MS nutrient solution used in the study not only contained nitrogen, phosphorus, and potassium, but also added amino acids such as glycine and inositol, as well as physiologically active substances. The comparison revealed that the treatment groups with added ingredients exhibited more complete root development, with increased taproot length and a significantly greater number of lateral roots. Furthermore, the article also mentioned that the root system of clump-forming bamboo is extremely sensitive to the osmotic pressure of the nutrient solution.In the experiment, when the osmotic pressure of the nutrient solution exceeded 0.4 MPa (such as in a high-concentration basic mineral nutrient solution), root water imbalance led to a 20-30% decrease in rooting rate. However, by gradually reducing the mannitol concentration (from 0.6 mol / L to 0.4 mol / L), the protoplast division rate increased from 30% to 45%, and root development was more robust. This indicates that while traditional basic mineral nutrient solutions provide essential elements such as nitrogen, phosphorus, and potassium, they fail to fully meet the needs of bamboo plants for trace elements, amino acids, and physiologically active substances during rooting, resulting in slow rooting and incomplete root development. The lack of an effective osmotic regulation mechanism in the nutrient solution easily leads to root water imbalance, thus affecting rooting efficiency. The article "Establishment of a Rapid Propagation System for Tissue Culture of *Phyllostachys pubescens*" (from the World Bamboo and Rattan Newsletter, Issue 1, 2022) uses *Phyllostachys pubescens* as material. By comparing different hormone combinations and nutrient solution formulations, it demonstrates that comprehensive regulation is superior to single treatment: 1. Synergistic effect of compound hormones: In the adventitious bud induction stage, the combination of 6-BA (3.00 mg / L) and TDZ (0.05 mg / L) induced 4.80 adventitious buds per explant, significantly higher than single hormone treatment. This indicates that the synergistic effect of multiple growth regulators can overcome the limitations of single hormones. 2. Nutritional synergy in the rooting stage: In the rooting medium, the combination of NAA (0.10 mg / L) and 6-BA (0.30 mg / L) resulted in a 100% rooting rate for tissue culture seedlings, with an average of 1.70 roots, a root length of 1.30 cm, and robust root systems with strong resistance. In contrast, while using only NAA (0.50 mg / L) achieved a 100% rooting rate, the roots were thin and the mortality rate after transplanting was high. This indicates that the synergistic effect of multiple growth regulators can better promote root development in bamboo plants and reduce mortality.

[0006] Furthermore, traditional nutrient solutions for promoting root growth in clump-forming bamboo often involve mixing various components in a single step, which can easily lead to problems such as metal ion precipitation and insufficient dissolution of polymers. This results in poor stability of the nutrient solution and reduced activity of the active ingredients. Therefore, researching and developing a nutrient solution that is nutritionally comprehensive, functionally synergistic, and highly stable for promoting root growth in clump-forming bamboo, along with its preparation process, is of great significance for improving the propagation efficiency and planting benefits of clump-forming bamboo. Summary of the Invention

[0007] The purpose of this invention is to provide a nutrient solution for promoting root growth of clump-forming bamboo and its preparation process, addressing the shortcomings of existing technologies.

[0008] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0009] This invention provides a nutrient solution for promoting root growth in clump-forming bamboo, comprising the following components in parts by weight:

[0010] 1-2 parts macroelements, 0.4-0.8 parts microelements, 0.001-0.003 parts amino acids, 0.002-0.004 parts plant growth hormones, 0.2-0.5 parts sugars, 0.1-0.2 parts vitamins, 0.0015-0.002 parts osmotic regulators, 0.3-0.4 parts chitosan, 0.08-0.12 parts polyacrylamide, 0.001-0.003 parts nanomaterials, and 10-30 parts water.

[0011] Preferably, the macroelements include ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate;

[0012] The mass ratio of ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate is 2-3:15-25:5-12:5-10:3-10.

[0013] Preferably, the trace elements include manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate; the mass ratio of manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate is 1–2: 0.5–0.8: 0.4–0.6: 2–4: 0.1–0.3: 0.01–0.05: 0.2–0.4.

[0014] Preferably, the amino acid is one or more of glycine, glutamic acid and phenylalanine; the sugar is glucose and / or sucrose.

[0015] Preferably, the plant growth hormone is aminoethyl ester, brassinolide, naphthaleneacetic acid, or indolebutyric acid; the osmotic regulator is one or more of mannitol, sorbitol, and polyethylene glycol.

[0016] Preferably, the vitamin is one or more of vitamin B1, vitamin B6 and vitamin E.

[0017] Preferably, the nanomaterial is nano-silicon or nano-calcium.

[0018] The present invention also provides a process for preparing the nutrient solution for promoting root growth of clump-forming bamboo, comprising the following steps:

[0019] 1) Mix a large amount of elements with some water to obtain solution A;

[0020] 3) Mix the trace elements and the remaining water to obtain solution B;

[0021] 3) Mix solution A and solution B, and add amino acids, plant growth hormone and polyacrylamide to the mixture in sequence to obtain solution C;

[0022] 4) Mix solution C, sugars, vitamins, osmotic regulators, chitosan and nanomaterials to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0023] Preferably, the mixing speed in steps 1) to 4) is 400 to 600 r / min, the mixing temperature is 20 to 30°C, and the mixing time is 25 to 30 min.

[0024] Preferably, the mass ratio of the water in step 1) to the remaining water in step 2) is 2-3:1-2.

[0025] The beneficial effects of this invention include the following:

[0026] 1) The nutrient solution described in this invention provides a balanced supply of macro-elements and a precise ratio of micro-elements. It also introduces various components such as chitosan, polyacrylamide, osmotic regulators, vitamins, amino acids, and nanomaterials to form a composite system of "nutrient supply + hormone regulation + structural support + osmotic protection", which is beneficial to improving the rooting efficiency of clump-forming bamboo.

[0027] 2) The nutrient solution described in this invention has a reasonable combination of substances, which can provide comprehensive nutrients for clumping bamboo, enabling it to take root quickly, significantly improving its rooting rate and growth rate, enhancing the resistance of bamboo seedlings, and enabling it to better adapt to harsh environmental conditions. It is an ideal promoter for the propagation and cultivation of clumping bamboo.

[0028] 3) Under different adverse conditions (such as drought, high temperature, low temperature, salinity, etc.), the clump-forming bamboo seedlings using the nutrient solution described in this invention not only have a high rooting rate and survival rate, but also show good development in terms of growth height, number of leaves, and root length, and have strong stress resistance and adaptability.

[0029] 4) The preparation process of this invention adopts a segmented dissolution method, which can avoid the formation of precipitates between metal ions (such as calcium and magnesium) and phosphate ions, ensuring the uniformity and stability of the nutrient solution. Moreover, the preparation process is simple and suitable for mass production and wide application. Detailed Implementation

[0030] This invention provides a nutrient solution for promoting root growth in clump-forming bamboo, comprising the following components in parts by weight:

[0031] 1-2 parts macroelements, 0.4-0.8 parts microelements, 0.001-0.003 parts amino acids, 0.002-0.004 parts plant growth hormones, 0.2-0.5 parts sugars, 0.1-0.2 parts vitamins, 0.0015-0.002 parts osmotic regulators, 0.3-0.4 parts chitosan, 0.08-0.12 parts polyacrylamide, 0.001-0.003 parts nanomaterials, and 10-30 parts water.

[0032] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 1 to 2 parts of macroelements, preferably 1.2 to 1.8 parts, more preferably 1.4 to 1.6 parts, and even more preferably 1.5 parts.

[0033] In this invention, the macroelements preferably include ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate;

[0034] The preferred mass ratio of ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate is 2-3:15-25:5-12:5-10:3-10, more preferably 2.4-2.6:18-22:7-10:6-8:5-8, and even more preferably 2.5:20:9:7:6.

[0035] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.4 to 0.8 parts of trace elements, preferably 0.5 to 0.7 parts, and more preferably 0.6 parts.

[0036] In this invention, the trace elements preferably include manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate;

[0037] The preferred mass ratio of manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate is 1–2:0.5–0.8:0.4–0.6:2–4:0.1–0.3:0.01–0.05:0.2–0.4, more preferably 1.4–1.6:0.6–0.7:0.45–0.55:2.5–3.5:0.15–0.25:0.02–0.04:0.25–0.35, and even more preferably 1.5:0.65:0.5:3:0.2:0.03:0.3.

[0038] In this invention, the ratio of ferrous sulfate to manganese sulfate in the trace elements is 2-4:1-2, and the iron content is relatively high, which can prevent yellowing caused by insufficient chlorophyll synthesis during the rooting process of clump bamboo.

[0039] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.001 to 0.003 parts of amino acids, preferably 0.0015 to 0.0025 parts, and more preferably 0.002 parts.

[0040] In this invention, the macroelements are the "building materials" of the root system, supporting the rapid growth and differentiation of the roots; the microelements are the "regulators" of the root system, activating metabolic processes and enhancing the physiological functions of the roots. Both types of elements are indispensable. Improper ratios or lack of certain elements will result in slow rooting, weak or deformed roots. It is necessary to reasonably match the types, concentrations and forms of elements.

[0041] In this invention, the amino acid is preferably one or more of glycine, glutamic acid, and phenylalanine.

[0042] In this invention, the amino acids can promote the development of the root system of clump-forming bamboo, improve stress resistance, and promote nutrient absorption.

[0043] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.2 to 0.5 parts of sugar, preferably 0.3 to 0.4 parts, and more preferably 0.35 parts.

[0044] In this invention, the sugars are preferably glucose and / or sucrose.

[0045] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.002 to 0.004 parts of plant growth hormone, preferably 0.0025 to 0.0035 parts, and more preferably 0.003 parts.

[0046] In this invention, the plant growth hormone is preferably aminoethyl ester, brassinolide, naphthaleneacetic acid, or indolebutyric acid.

[0047] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.0015 to 0.002 parts of an osmosis regulator, preferably 0.0016 to 0.0018 parts, and more preferably 0.0017 parts.

[0048] In this invention, the osmosis regulator is preferably one or more of mannitol, sorbitol, and polyethylene glycol.

[0049] In this invention, the osmotic regulator can regulate the osmotic pressure around the roots, prevent water loss, and adapt to the physiological characteristics of the clump-forming bamboo root system.

[0050] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.1 to 0.2 parts of vitamins, preferably 0.12 to 0.18 parts, more preferably 0.14 to 0.16 parts, and even more preferably 0.15 parts.

[0051] In this invention, the vitamin is preferably one or more of vitamin B1, vitamin B6 and vitamin E.

[0052] In this invention, the vitamins can directly participate in the metabolism of root cells of clump-forming bamboo, accelerating its rooting process.

[0053] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.3 to 0.4 parts of chitosan, preferably 0.32 to 0.38 parts, more preferably 0.34 to 0.36 parts, and even more preferably 0.35 parts.

[0054] In this invention, the chitosan has biocompatibility and antibacterial properties, which can protect the roots of clump-forming bamboo from microbial attack.

[0055] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.08 to 0.12 parts of polyacrylamide, preferably 0.09 to 0.11 parts, and more preferably 0.1 parts.

[0056] In this invention, the polyacrylamide, as a high molecular polymer, can increase the viscosity of the nutrient solution, prolong the contact time of the roots of clump-forming bamboo, and improve the nutrient adsorption efficiency.

[0057] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 0.001 to 0.003 parts of nanomaterials, preferably 0.0015 to 0.0025 parts, and more preferably 0.002 parts.

[0058] In this invention, the nanomaterial is preferably nano-silicon or nano-calcium.

[0059] In this invention, the nanomaterial can enhance the absorption rate of nutrients by bamboo seedlings, help them absorb water and mineral elements more effectively, improve the stability of nutrient solution and the growth rate of plants, and enhance their stress resistance.

[0060] The nutrient solution for promoting root growth of clump-forming bamboo of the present invention contains 10-30 parts water, preferably 15-25 parts, more preferably 18-22 parts, and even more preferably 20 parts.

[0061] The present invention also provides a process for preparing the nutrient solution for promoting root growth of clump-forming bamboo, comprising the following steps:

[0062] 1) Mix a large amount of elements with some water to obtain solution A;

[0063] 2) Mix the trace elements and the remaining water to obtain solution B;

[0064] 3) Mix solution A and solution B, and add amino acids, plant growth hormone and polyacrylamide to the mixture in sequence to obtain solution C;

[0065] 4) Mix solution C, sugars, vitamins, osmotic regulators, chitosan and nanomaterials to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0066] In this invention, the mixing speed in steps 1) to 4) is preferably 400 to 600 r / min, more preferably 450 to 550 r / min, and even more preferably 500 r / min; the mixing temperature is preferably 20 to 30°C, more preferably 25°C; and the mixing time is preferably 25 to 30 min, more preferably 26 to 28 min, and even more preferably 27 min.

[0067] In this invention, the mass ratio of the partial water in step 1) to the remaining water in step 2) is preferably 2-3:1-2, more preferably 2.4-2.6:1.4-1.6, and even more preferably 2.5:1.5.

[0068] In this invention, by optimizing the mixing speed, mixing temperature and mixing time, it is possible to ensure that the polymer (polyacrylamide) is fully dissolved, while avoiding excessively high temperatures that could lead to the inactivation of plant growth hormones (e.g., indolebutyric acid).

[0069] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0070] The nano-silicon used in the examples is nano-silicon element liquid fertilizer (Silicon Gold Treasure) produced by Jiangsu Shidai Jingu Technology Co., Ltd.; the nano-calcium is nano-suspended calcium produced by Lianyungang Weinuo Biotechnology Co., Ltd.

[0071] Example 1

[0072] Mix 100g of macroelements (including 4g ammonium nitrate, 32g potassium nitrate, 24g calcium chloride, 20g magnesium sulfate, and 20g sodium dihydrogen phosphate) and 600g of deionized water in a mixer at 20℃ and 500r / min for 25min to obtain solution A. Mix 40g of microelements (including 10g manganese sulfate, 4g zinc sulfate, 3g copper sulfate, 20g ferrous sulfate, 1.5g cobalt chloride, 0.25g boric acid, and 1.25g sodium molybdate) and 400g of deionized water in a mixer at 25℃ and 600r / min for 30min to obtain solution B. Mix solutions A and B in a mixer at 30℃ and 500r / min for 25min. Then, sequentially add 0.1g glycine, 0.2g amino ester, and 8g polyacrylamide to the mixture and stir until homogeneous to obtain solution C.

[0073] Solution C, 20g sucrose, 10g vitamin B1, 0.15g mannitol, 30g chitosan and 0.1g nano-silicon were mixed in a mixer at 20℃ and 400r / min for 28min to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0074] Example 2

[0075] Mix 150g of macroelements (including 9g ammonium nitrate, 75g potassium nitrate, 24g calcium chloride, 15g magnesium sulfate, and 27g sodium dihydrogen phosphate) and 1000g of deionized water in a mixer at 25℃ and 400r / min for 30min to obtain solution A. Mix 60g of microelements (including 12g manganese sulfate, 7.2g zinc sulfate, 4.8g copper sulfate, 30g ferrous sulfate, 2.4g cobalt chloride, 0.24g boric acid, and 3.36g sodium molybdate) and 1000g of deionized water in a mixer at 30℃ and 500r / min for 25min to obtain solution B. Mix solutions A and B in a mixer at 20℃ and 600r / min for 26min. Then, sequentially add 0.2g glutamic acid, 0.3g brassinolide, and 10g polyacrylamide to the mixture and stir until homogeneous to obtain solution C.

[0076] Solution C, 40g glucose, 15g vitamin B6, 0.18g sorbitol, 35g chitosan and 0.2g nano-silicon were mixed in a mixer at 25℃ and 500r / min for 25min to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0077] Example 3

[0078] 200g of macroelements (including 10g ammonium nitrate, 100g potassium nitrate, 43g calcium chloride, 30g magnesium sulfate, and 17g sodium dihydrogen phosphate) and 2000g of deionized water were mixed in a mixer at 30℃ and 600r / min for 28min to obtain solution A. 80g of microelements (including 20g manganese sulfate, 8g zinc sulfate, 6g copper sulfate, 40g ferrous sulfate, 3g cobalt chloride, 0.5g boric acid, and 2.5g sodium molybdate) and 1000g of deionized water were mixed in a mixer at 20℃ and 400r / min for 28min to obtain solution B. Solutions A and B were mixed in a mixer at 25℃ and 400r / min for 30min. Then, 0.3g phenylalanine, 0.4g naphthaleneacetic acid, and 12g polyacrylamide were added sequentially to the mixture and stirred until homogeneous to obtain solution C.

[0079] Solution C, 50g sucrose, 20g vitamin E, 0.2g polyethylene glycol, 40g chitosan and 0.3g nano-silicon were mixed in a mixer at 30℃ and 600r / min for 30min to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0080] Example 4

[0081] 120g of macroelements (including 6g ammonium nitrate, 45g potassium nitrate, 15g calcium chloride, 24g magnesium sulfate, and 30g sodium dihydrogen phosphate) and 1500g of deionized water were mixed in a mixer at 30℃ and 600 rpm for 26 min to obtain solution A. 60g of microelements (including 10g manganese sulfate, 8g zinc sulfate, 4g copper sulfate, 32g ferrous sulfate, 2g cobalt chloride, 0.5g boric acid, and 3.5g sodium molybdate) and 500g of deionized water were mixed in a mixer at 20℃ and 400 rpm for 25 min to obtain solution B. Solutions A and B were mixed in a mixer at 25℃ and 400 rpm for 30 min. Then, 0.25g phenylalanine, 0.25g indolebutyric acid, and 10g polyacrylamide were added sequentially to the mixture and stirred until homogeneous to obtain solution C.

[0082] Solution C, 35g sucrose, 15g vitamin E, 0.18g mannitol, 35g chitosan and 0.15g nano calcium were mixed in a mixer at 30℃ and 600r / min for 25min to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

[0083] Example 5

[0084] 180g of macroelements (including 9g ammonium nitrate, 75g potassium nitrate, 36g calcium chloride, 30g magnesium sulfate, and 30g sodium dihydrogen phosphate) and 900g of deionized water were mixed in a mixer at 30℃ and 550 rpm for 24 min to obtain solution A. 50g of microelements (including 10g manganese sulfate, 8g zinc sulfate, 5g copper sulfate, 20g ferrous sulfate, 3g cobalt chloride, 0.5g boric acid, and 3.5g sodium molybdate) and 900g of deionized water were mixed in a mixer at 25℃ and 450 rpm for 25 min to obtain solution B. Solutions A and B were mixed in a mixer at 25℃ and 400 rpm for 30 min. Then, 0.28g phenylalanine, 0.3g indolebutyric acid, and 10g polyacrylamide were added sequentially to the mixture and stirred until homogeneous to obtain solution C.

[0085] Solution C, 38g sucrose, 15g vitamin E, 0.1g mannitol, 0.1g sorbitol, 35g chitosan and 0.12g nano calcium were mixed in a mixer at 30℃ and 600r / min for 25min to obtain a nutrient solution that promotes rooting of clump-forming bamboo.

[0086] Comparative Example 1

[0087] In Example 1, the mass of ammonium nitrate in the macroelements was modified to 32g, the mass of potassium nitrate was modified to 4g, mannitol was omitted, and other conditions remained unchanged, resulting in a nutrient solution that promotes root growth in clump-forming bamboo.

[0088] Comparative Example 2

[0089] In Example 2, the mass of manganese sulfate in the trace elements was modified to 30g, the mass of ferrous sulfate was modified to 12g, glutamic acid and chitosan were omitted, and other conditions remained unchanged, to obtain a nutrient solution that promotes rooting of clump-forming bamboo.

[0090] Comparative Example 3

[0091] Omit the sucrose and naphthaleneacetic acid from Example 3, adjust the mixing temperature of solution A and solution B to 50°C, adjust the mixing time to 5 min, and keep other conditions unchanged to obtain a nutrient solution that promotes rooting of clump-forming bamboo.

[0092] Comparative Example 4

[0093] The polyacrylamide in Example 4 was omitted, the mass of vitamin E was changed to 0.5g, the method of adding the feed in stages was changed to one-time mixing, and other conditions remained unchanged, to obtain a nutrient solution that promotes the rooting of clump-forming bamboo.

[0094] Comparative Example 5

[0095] The indolebutyric acid in Example 5 was omitted. The mixing temperature of solution A and solution B was adjusted to 5°C and the mixing time was adjusted to 60 min, while other conditions remained unchanged, to obtain a nutrient solution that promotes rooting of clump-forming bamboo.

[0096] Comparative Example 6

[0097] Indolebutyric acid with the same mass as the nutrient solution in Example 1.

[0098] Comparative Example 7

[0099] The glycine, amino acid ester, polyacrylamide, sucrose, chitosan, vitamin B1, mannitol and nano-silicon in Example 1 are omitted, while other conditions remain unchanged.

[0100] Comparative Example 8

[0101] The mannitol and nano-silicon in Example 1 were omitted. The mixing speed of solution A and solution B was adjusted to 900 r / min and the mixing time was adjusted to 5 min, while other conditions remained unchanged.

[0102] (I) Verifying the rooting effects of different nutrient solutions on clump-forming bamboo

[0103] Select green bamboo cuttings with uniform growth, cut them into 40cm sections, bundle 50 cuttings together, rinse with running water for 30 minutes, then disinfect the surface with an 80% ethanol solution. Next, remove the cuttings and immerse them in a 20% mercuric chloride solution for further disinfection. After disinfection, retain 1-2 buds on each cutting, cut the bottom flat, and insert 100 cuttings evenly into the nutrient solutions prepared in Examples 1-5 and Comparative Examples 1-8, with a distance of 10cm between adjacent cuttings. Cultivate for 20 days under the following environmental conditions: temperature 25℃, humidity 82%, light intensity 2000 Lux, and light duration 6h / d. Remove diseased and rotten plants promptly during cultivation. Rooting rate, average number of roots, and average root length are then statistically analyzed, and the results are shown in Table 1.

[0104] Rooting rate = number of rooted cuttings / total number of cuttings × 100%; average number of roots = total number of rooted cuttings / total number of rooted cuttings. The statistics of rooting rate and average number of roots are compiled by professional technicians.

[0105] Table 1. Rooting status of green bamboo

[0106]

[0107] As shown in Table 1, the nutrient solution for promoting root growth of clump-forming bamboo in this invention provides a balanced supply of macro-elements, a precise ratio of micro-elements, and introduces additional components such as chitosan, polyacrylamide, osmotic regulators, vitamins, and amino acids to form a composite system of "nutrient supply + hormone regulation + structural support + osmotic protection," which is beneficial to improving the rooting efficiency of clump-forming bamboo.

[0108] The nutrient solution of this invention has a reasonable combination of substances, which can provide comprehensive nutrients for clumping bamboo, enabling it to take root quickly and significantly improving its rooting rate and growth speed.

[0109] As can be seen from Example 1 and Comparative Example 1, in this invention, the ratio of high potassium and low nitrogen in macronutrients can promote the elongation and differentiation of root cells in clump-forming bamboo.

[0110] As can be seen from Example 2 and Comparative Example 2, in this invention, the precise proportion of each component in the trace elements, as well as the addition of amino acids and chitosan, can promote the rooting of clump-forming bamboo.

[0111] As can be seen from Example 3 and Comparative Example 3, in this invention, the combination of plant growth hormone and sugar can both induce root primordia differentiation through hormones and provide energy through sugars, thereby accelerating the development of the root system of clump-forming bamboo.

[0112] As can be seen from Example 4 and Comparative Example 4, the preparation process of the present invention adopts a segmented dissolution method, which can avoid the formation of precipitates between metal ions (such as calcium and magnesium) and phosphate ions, ensure the uniformity and stability of the nutrient solution, and help improve the rooting efficiency of clump bamboo.

[0113] As can be seen from Example 5 and Comparative Example 5, the application of plant growth hormones in rooting nutrient solution can promote root formation and growth.

[0114] As can be seen from Example 1 and Comparative Examples 6-7, single plant growth hormones and traditional basic mineral nutrient solutions lack comprehensive nutritional support, resulting in slow rooting speed and incomplete root development.

[0115] As shown in Example 1 and Comparative Example 8, nanomaterials can enhance the absorption rate of nutrients by bamboo seedlings, help them absorb water and mineral elements more effectively, improve the stability of nutrient solution and the growth rate of plants, and increase their rooting rate.

[0116] (II) Verifying the survival rate of rooted clump-forming bamboo seedlings after transplanting

[0117] The rooted green bamboo seedlings of Examples 1-5 and Comparative Examples 1-8 were moved to natural room temperature for 7 days to harden off. Then, the seedlings were soaked in 1000 times diluted carbendazim solution for 5 hours and transplanted onto a "coconut coir + river sand + soil" substrate with 80% shading and kept moist. The transplant survival rate was counted after one week. The results are shown in Table 2.

[0118] Table 2 Survival rate of green bamboo seedlings after transplanting

[0119]

[0120]

[0121] (III) Conduct stress resistance and adaptability tests on rooted clump-forming bamboo seedlings.

[0122] 1. Drought resistance test

[0123] The rooted green bamboo seedlings of Examples 1-5 were divided into 4 groups of 20 plants each. They were then placed in a control group (normal water supply), a mild drought group (watered once every 2 days), a moderate drought group (watered once every 4 days), and a severe drought group (watered once every 7 days). The growth status of the bamboo seedlings was recorded after 14 days, and the results are shown in Table 3.

[0124] Table 3. Results of drought resistance test on green bamboo seedlings

[0125]

[0126]

[0127] 2. High temperature resistance test

[0128] The rooted green bamboo seedlings of Examples 1-5 were divided into 3 groups of 20 plants each. They were then placed in the control group (room temperature), high temperature group 1 (30℃), and high temperature group 2 (35℃). After 7 days, the growth status of the bamboo seedlings was recorded. The results are shown in Table 4.

[0129] Table 4. Results of the high-temperature resistance test for green bamboo seedlings

[0130]

[0131]

[0132] 3. Cold resistance test

[0133] The rooted green bamboo seedlings of Examples 1-5 were divided into 4 groups of 20 plants each. They were then placed in the control group (room temperature), low temperature group 1 (15℃), low temperature group 2 (10℃), and low temperature group 3 (5℃). After 7 days, the growth status of the bamboo seedlings was recorded. The results are shown in Table 5.

[0134] Table 5 Results of cold resistance test for green bamboo seedlings

[0135]

[0136]

[0137] 4. Salt and alkali resistance test

[0138] The rooted green bamboo seedlings of Examples 1-5 were divided into 3 groups of 20 plants each. They were then placed in the control group (ordinary soil), the low-salt-alkali group (ordinary soil + 1% sodium chloride solution), and the high-salt-alkali group (ordinary soil + 3% sodium chloride solution). The growth status of the bamboo seedlings was recorded after 14 days. The results are shown in Table 6.

[0139] Table 6 Results of salt and alkali tolerance test for green bamboo seedlings

[0140]

[0141] As shown in Tables 2-6, the nutrient solution for promoting root growth of clump-forming bamboo described in this invention exhibits significant effects. Under different adverse conditions (e.g., drought, high temperature, low temperature, salinity, etc.), clump-forming bamboo seedlings using this nutrient solution not only show higher rooting and survival rates but also demonstrate better development in terms of growth height, number of leaves, and root length. Furthermore, when bamboo seedlings encounter environmental stress, plants using this nutrient solution exhibit stronger resistance and adaptability.

[0142] The nutrient solution of this invention can significantly improve the rooting effect, survival rate and stress resistance of clumping bamboo, and is an ideal promoter for the propagation and cultivation of clumping bamboo.

[0143] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A nutrient solution for promoting root growth in clump-forming bamboo, characterized in that, The components comprise the following parts by weight: 1-2 parts macroelements, 0.4-0.8 parts microelements, 0.001-0.003 parts amino acids, 0.002-0.004 parts plant growth hormones, 0.2-0.5 parts sugars, 0.1-0.2 parts vitamins, 0.0015-0.002 parts osmotic regulators, 0.3-0.4 parts chitosan, 0.08-0.12 parts polyacrylamide, 0.001-0.003 parts nanomaterials, and 10-30 parts water.

2. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 1, characterized in that, The macroelements include ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate; The mass ratio of ammonium nitrate, potassium nitrate, calcium chloride, magnesium sulfate, and sodium dihydrogen phosphate is 2-3:15-25:5-12:5-10:3-10.

3. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 1 or 2, characterized in that, The trace elements include manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate. The mass ratio of manganese sulfate, zinc sulfate, copper sulfate, ferrous sulfate, cobalt chloride, boric acid, and sodium molybdate is 1–2: 0.5–0.8: 0.4–0.6: 2–4: 0.1–0.3: 0.01–0.05: 0.2–0.

4.

4. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 3, characterized in that, The amino acid is one or more of glycine, glutamic acid and phenylalanine; The sugars are glucose and / or sucrose.

5. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 4, characterized in that, The plant growth hormone is amino acid ester, brassinolide, naphthaleneacetic acid, or indolebutyric acid; The osmotic regulator is one or more of mannitol, sorbitol, and polyethylene glycol.

6. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 4 or 5, characterized in that, The vitamin mentioned is one or more of vitamin B1, vitamin B6, and vitamin E.

7. The nutrient solution for promoting root growth of clump-forming bamboo according to claim 6, characterized in that, The nanomaterial is nano-silicon or nano-calcium.

8. The preparation process of the nutrient solution for promoting root growth of clump-forming bamboo according to any one of claims 1 to 7, characterized in that, It includes the following steps: 1) Mix a large amount of elements with some water to obtain solution A; 2) Mix the trace elements and the remaining water to obtain solution B; 3) Mix solution A and solution B, and add amino acids, plant growth hormone and polyacrylamide to the mixture in sequence to obtain solution C; 4) Mix solution C, sugars, vitamins, osmotic regulators, chitosan and nanomaterials to obtain a nutrient solution that promotes root growth in clump-forming bamboo.

9. The preparation process according to claim 8, characterized in that, The mixing speed in steps 1) to 4) is 400 to 600 r / min, the mixing temperature is 20 to 30 °C, and the mixing time is 25 to 30 min.

10. The preparation process according to claim 9, characterized in that, The mass ratio of the water in step 1) to the remaining water in step 2) is 2-3:1-2.

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