Method for rapidly growing and cultivating dendrobium officinale

Abstract

The invention discloses a method for rapidly growing and cultivating dendrobium officinale, which comprises the following steps of: taking back mature shadow fruit seeds in the country of origin for wild dendrobium officinale, and placing the seeds in a clean culture dish; after natural crack, pouring the seeds into a clean beaker, sealing the clean beaker, and storing the beaker in a low temperature box; performing a certain sterilization procedure on the stored seeds, placing the seeds in an MS minimal medium, performing seed germination cultivation, wherein the seeds gradually become green and form protocorm, and observing that needles appear in the protocorm by using magnifying glass; transferring the protocorm into the MS minimal medium, performing protocorm proliferation and differentiation culture, observing that plantlets are differentiated from the protocorm in a bottle along the production of partial new protocorm; and transferring the plantlets into the MS minimal medium, and performing strong seedling and rooting culture, seedling hardening and transplanting. The survival rate is over 97 percent without the seedling hardening process, and the yield is doubled. The growth speed is improved by 50 percent, the content of each component is equivalent to that of the wild dendrobium officinale, and the contents of amino acid and microelement are higher than those of the wild dendrobium officinale.

Description

technical field [0001] The invention belongs to the field of propagation of dendrobium candidum, and more specifically relates to a method for rapid growth and cultivation of dendrobium candidum. Background technique [0002] Dendrobium officinale is commonly known as human fairy grass. Its main components are alkaloids and polysaccharides. It is also rich in amino acids and trace elements. Modern clinical applications and medicinal studies have confirmed that Dendrobium officinale can treat throat diseases, gastrointestinal diseases, cataracts, and cardiovascular diseases. , diabetes and inhibition of tumor growth have significant curative effects. The demand in the domestic and foreign markets continues to increase, and the price has soared. Due to the slow growth and low output of wild Dendrobium candidum, coupled with people's continuous digging, wild Dendrobium candidum is facing a state of extinction. It cannot meet the market demand. The Chinese pharmaceutical industr...

AI Technical Summary

Problems solved by technology

[0002] Dendrobium officinale is commonly known as immortal grass in the world. Its main components are alkaloids and polysaccharides. It is also rich in amino acids and trace elements. Modern clinical application and medicinal research have confirmed that Dendrobium officinale can treat throat diseases, gastrointestinal diseases, cataracts, and cardiovascular diseases. , diabetes and inhibition of tumor growth have significant curative effects. The demand in the domestic and foreign markets continues to increase, and the price has soared. Due to the slow growth and low output of wild Dendrobium candidum, coupled with people's continuous digging, wild Dendrobium candidum is facing extinction. It cannot meet the market demand. The Chinese pharmaceutical industry lists Dendrobium candidum as a kind of key protected rare and endangered species, and encourages artificial large-scale cultivation of Dendrobium candidum. At present, some scientific research institutions and business units in China are focusing on tissue culture and artificial large-scale cultivation of Dendrobium candidum Cultivation techniques have been explored and tested, and some achievements have been made. However, raw materials such as chemicals, chemicals, and pesticides were used during the tissue culture and cultivation period, and there are still problems such as slow propagation speed of Dendrobium candidum seedlings, and the survival rate of tissue cultured seedlings and trained seedlings transplanted. The main problems such as low growth rate, low yield, residual chemical components, and low effective components have seriously restricted the industrialization and large-scale development of Dendrobium officinale.