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Method for cross breeding and seedling propagation of paphiopedilum

A hybrid breeding and seedling technology, applied in horticultural methods, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of incomplete embryo development, low germination rate, and difficulty in forming seedlings of hybrid seeds of Paphiopedilum chinensis, and achieve germination. The effect of high rate, good seedling quality and fast seedling growth

Inactive Publication Date: 2009-11-04
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the incomplete development of the embryo, the hybrid seeds of Paphiopedilum are extremely difficult to germinate in the natural state, and there are also difficulties such as low germination rate and difficult seedlings when using ordinary aseptic sowing techniques.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Hybrid breeding and seedling propagation of P. malipoense and P. sukhakulii:

[0021] (1) Artificial pollination: Two kinds of Paphiopediopsis malipopsis and Paphiopedilum sushii bloom at the same time from December to March of the next year, and artificial pollination is carried out 6 days after the flowers unfold. Before pollination, the female parent and father are removed. The lip petals of the parent pollinate the pollen of the male parent to the stigma of the female parent, and the two parents perform reciprocal crossing as the father and female parent. After pollination, a label should be hung, and the label has information such as the name of the parent, the name of the mother, the date of hybridization, and the pollinator. The success rate of pollination was counted at 3 months, and there was no difference between reciprocal and reciprocal crosses, and the seed setting rate reached more than 80%;

[0022] (2) Aseptic sowing: the fruit 6 months after pollinatio...

Embodiment 2

[0026] Embodiment 2: the crossbreeding and seedling propagation of P. wardii and P. armeniacum

[0027] (1) Artificial pollination: P. chinensis and P. apricotii bloom at the same time in February, and artificial pollination is carried out 10 days after the flowers unfold. On the stigma of the original, the two parents are reciprocal crosses as father and mother. After pollination, a label should be hung, and the label has information such as the name of the parent, the name of the mother, the date of hybridization, and the pollinator. The success rate of pollination was counted at 3 months, and there was no difference between reciprocal and reciprocal crosses, and the seed setting rate reached more than 70%;

[0028] (2) Aseptic sowing: the fruit 5 months after pollination was first soaked in 80% alcohol for 60 seconds and then disinfected in 0.2% mercury solution for 20 minutes, rinsed with sterile water for 5 times and cut the fruit. The powdery embryos are inoculated on ...

Embodiment 3

[0032] Embodiment 3: the crossbreeding and the seedling propagation of P.Maudiae and P.callosum

[0033] (1) Artificial pollination: Paphiopedilum tumefaciens used in this experiment bloomed in October, while Paphiopedilum mortifolia bloomed in March of the following year. When Paphiopedilum papillium blooms, its pollen is stored in a sealed and sterilized plastic tube in a refrigerator at 4°C. After 5 months, when Paphiopedilum is in bloom, the pollen preserved at low temperature is stored in a humid room temperature ( The temperature is 20-30°C, the humidity is not less than 75%) and placed for 30 minutes, and then the viability of the pollen is measured by the triphenyltetrazolium chloride staining method, and all the pollen is found to be viable, and it is given to the stigma of Paphiopedilum superior. Tags are hung after pollination, and the tags have information such as the names of the parents, the names of the mothers, the date of hybridization and the pollinator. Th...

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Abstract

The invention relates to a method for cross breeding and seedling propagation of paphiopedilum, which comprises the steps of artificial pollination, sterile seeding, strong seedling culture, and test-tube plantlet transplant. After the artificial pollination of paphiopedilum coenospecies, a powdery embryo in hybrid fruits is inoculated to a specially prepared seed germination culture medium for culture; plantlets obtained by the sterile seeding are inoculated to a specially prepared strong seedling culture medium for strong seedling culture; and finally, the test-tube plantlet transplant is performed. The method uses unique culture media, ensures the high germination rate, quick seedling and high seedling quality of paphiopedilum hybrid seeds, has the advantages of high operability, high application value and the like, and overcomes the difficulty that the paphiopedilum hybrid seeds are extremely difficult to germinate in a natural state due to the incomplete development of embryo and other difficulties.

Description

technical field [0001] The invention relates to a plant seedling propagation method, in particular to a method for hybrid breeding and seedling propagation of Paphiopedilum. Background technique [0002] Paphiopedilum is also called slipper orchid, cinderella, etc. There are about 77 species in the world, epiphytic or terrestrial, and they are mainly distributed in tropical Asia to Pacific islands. There are 18 species recorded in the Flora of China, among which P. armeniacum and P. emersonii are endemic to my country. In recent years, P. spicerianum has been discovered. ), P. helenae (P.helenae) are also distributed in China. Paphiopedilum has a very high ornamental value due to its unique flower shape, gorgeous flower color, and long-lasting ornamental flowering period. It is a very popular high-end flower in the international flower market and has a large number of lovers in the world. However, due to the destructive excavation of wild resources due to people's excessive...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H4/00A01G31/00
Inventor 曾宋君陈之林段俊吴坤林
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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