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High-efficient propagation method of seedlings of Dendrobium officinale

A technology for Dendrobium officinale and seedlings is applied in the field of propagation of medicinal plant seedlings, which can solve the problems of lack of high-quality seedlings and the like, and achieve the effects of low cost, simple operation and shortening of maturity period.

Inactive Publication Date: 2009-06-24
SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, artificial planting of Dendrobium candidum has broad prospects, but the lack of high-quality seedlings is one of the bottlenecks in the planting industry of Dendrobium candidum

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 120 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 70% for 30 seconds, sterilized in a mercuric chloride solution with a mass fraction of 0.1% for 20 minutes, rinsed with sterile water for 4 times, then cut the fruit, and used an inoculation needle to inoculate the yellow-white Powdered embryos were inoculated into seed germination medium (the medium contained 1.5g of Huabao No. 1, 0.5g of peptone, 50mL of coconut milk, 15g of sucrose, 6g of agar, 1g of activated carbon, and the rest were vitamins and muscle in MS medium. Alcohol component), seeds germinated after 10 days to form protocorms, and seedlings were formed after 80 days.

[0023] Transfer 100 seedlings with a height of 2 to 3 cm to the test tube flowering medium (each liter contains 0.5 mg of 6-benzylpurine (6-BA...

Embodiment 2

[0028] When flowering, a vigorous mother plant is selected for artificial pollination, and the fruit is ripe 110 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in alcohol with a volume fraction of 75% for 40 seconds, sterilized in a mercuric chloride solution with a mass fraction of 0.15% for 30 minutes, rinsed with sterile water for 5 times, then cut the fruit, and used an inoculation needle to inoculate the yellow-white Powdered embryos were inoculated into seed germination medium (each liter of the medium contained 1g of Huabao No. 1, 1g of peptone, 80mL of coconut milk, 25g of sucrose, 6.5g of agar, 0.8g of activated carbon, and the rest were vitamins and muscle in MS medium. Alcohol component), after 10 days, the seeds germinate and form protocorms. In order to obtain more seedlings in the production process, the protocorms are formed into mixed tissues of buds and protocorms. Bao No. 1 1g, peptone 0.5g, cocon...

Embodiment 3

[0034]When flowering, a strong mother plant is selected for artificial pollination, and the fruit is ripe 130 days after pollination, and the fruit is used as an explant for sowing. When sowing, the explants were soaked in 80% alcohol for 60 seconds, sterilized in 0.2% mercury solution for 30 minutes, rinsed with sterile water for 4 times, then cut the fruit, and inoculated the yellow-white Powdered embryos were inoculated into seed germination medium (the medium contained 1.5g of Huabao No. 1 per liter, 2g of peptone, 100mL of coconut milk, 30g of sucrose, 7g of agar, 1g of activated carbon, and the rest were vitamins and inositol in MS medium. Components), after 10 days, the seeds germinate and form protocorms. In order to obtain more seedlings during the production process, the protocorms are formed into a mixed tissue of buds and protocorms. No. 1 2g, peptone 2g, coconut juice 100mL, sucrose 20g, agar 7g, 6-benzylpurine 1.0mg, activated carbon 2g, and the rest are vitamins...

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Abstract

The invention relates to a propagating method of Dendrobium officinale Kimura et Migo seedlings, characterized in that, vigorous Dendrobium officinale Kimura et Migo maternal plant is performed with artificial pollination, the obtained fruit is sanitized and cut, seeds are used for bourgeoning culture medium, causing the seeds to bourgeon protocorm and further form young seedling, then the young seedling propagated in s test tube is cultivated by test tube blossom culture medium, the vigorous plant is selected with artificial pollination in the test tube after blossom, cut after fruit ripening with aseptic seeding, causing the seeds to develop into small plant, which is cultivated on good seedling culture medium, mature shoot is formed, the mature shoot is performed with seedling exercising in the natural light, furthermore cultivated into the seedling. The invention is simple in operation, low in cost, greatly shortened in maturing stage of the fruit of Dendrobium officinale Kimura et Migo, high in efficiency and no plant diseases and insect pests, and transplanting success ratio reaches more than 95%.

Description

technical field [0001] The invention relates to a method for propagating medicinal plant seedlings, in particular to a method for efficiently propagating seedlings of Dendrobium officinale Kimura et Migo through test tube flowering and pollination. Background technique [0002] Dendrobium officinale Kimura et Migo is a famous and precious traditional Chinese medicine commonly used in my country. It has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, moistening lung and relieving cough, etc. disease. Modern pharmacological studies have shown that Dendrobium also has the effects of anti-tumor, anti-aging, enhancing human immunity and dilating blood vessels. However, due to the depletion of natural resources due to long-term unrestrained excavation by people, and the extremely low reproductive rate of Dendrobium candidum itself, Dendrobium candidum has become one of the national key protected medicinal materials. Therefore artifi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/02C12N5/04
Inventor 陈之林曾宋君吴坤林郑枫段俊
Owner SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI
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