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Application of lactobacillus paracasei having antiallergic effect, functional food composition and preparation method of functional food composition

A technology for functional food and secondary cheese, which can be used in food preparation, medical preparations containing active ingredients, applications, etc., and can solve problems such as infant allergies

Active Publication Date: 2015-04-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] β-lactoglobulin has a function similar to immunoglobulin for calves, but it is the main allergen for infants, which is likely to cause allergies in infants (the digestive system of infants has not yet fully developed, β-lactoglobulin is more prone to " The whole "was absorbed and judged as a pathogen by the immune system)

Method used

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  • Application of lactobacillus paracasei having antiallergic effect, functional food composition and preparation method of functional food composition
  • Application of lactobacillus paracasei having antiallergic effect, functional food composition and preparation method of functional food composition
  • Application of lactobacillus paracasei having antiallergic effect, functional food composition and preparation method of functional food composition

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preparation example Construction

[0034] In the third aspect, the present invention also provides a method for preparing a functional food composition, the method comprising: adding the cells of Lactobacillus paracasei with the preservation number CGMCC No. 9800 to the food.

[0035] The method for preparing the functional food composition provided by the invention comprises adding the live and / or dead bacteria of Lactobacillus paracasei to food.

[0036]According to the present invention, the preparation method of the dead Lactobacillus paracasei is not particularly limited, for example, the above-mentioned cultured live Lactobacillus paracasei can be killed by heating or radiation. The conditions for killing by heating may include: the temperature is 65-85°C, and the time is 0.5-1.5h.

[0037] According to the present invention, although adding the thalline of the Lactobacillus paracasei to the food can achieve the purpose of the present invention, that is, after eating, it can regulate the immunity of the b...

Embodiment 1

[0046] This example illustrates the impact of CGMCC No.9800 on allergic reactions

[0047] 1.1 Establishment of animal model

[0048] Experimental animals: BALB / c mice, female, clean grade, 6-8 weeks old, purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd. They were raised in the animal room of the School of Food Science and Technology, China Agricultural University. The temperature of the feeding environment was kept at 25±2°C, the light was alternated every day for 12 hours, the mice were fed with standard mouse feed, and they had free access to drinking water. The method of random grouping was adopted to set up blank group (CON), allergy group (BLG), bacteria control group (D) and bacteria experimental group (A), with 8 mice in each group.

[0049] Cultivation of bacterial strains: the preservation number is CGMCC No.9800 and the control bacteria are cultured in MRS liquid medium, the culture condition is 37°C, 12 hours, after the culture, the bac...

Embodiment 2

[0076] This example illustrates the effect of CGMCC No.9800 on the differentiation and maturity of dendritic cells

[0077] Cultivation of bacterial strains: The preservation number is CGMCC No.9800 and the bacteria used for the control are all cultured in MRS liquid medium. The culture condition is 37 ° C for 12 hours. Lethal for 0.5 hours.

[0078] 2.1 Preparation of bone marrow-derived dendritic cells

[0079] Take BALB / c mice aged 6-8 weeks and weighing 18-22 g, half male and half male. They were sacrificed by dislocation of the cervical spine, and immediately immersed in 75% alcohol for 5 minutes. The femur and tibia were aseptically separated in an ultra-clean bench, and the muscles and tendons were removed as much as possible, and then placed in sterile PBS buffer solution with pH 7.2. Cut off both ends of the femur, draw 1 mL of RPMI 1640 culture solution with pH 7.2 with a sterile syringe, and wash the bone marrow, most of the bone marrow cells can be washed out. T...

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Abstract

The invention discloses application of lactobacillus paracasei of which the preservation number is CGMCC No.9800 in regulation of the immunologic function of the human body. The application does not include treatment on diseases of the human body or the animal body. The invention also discloses a functional food composition containing the lactobacillus paracasei and a preparation method of the functional food composition. Compared with other congeneric lactobacillus paracasei, the lactobacillus paracasei of which the preservation number is CGMCC No.9800 can be used for regulating the immunologic function of the human body more effectively; experiments show that the lactobacillus paracasei of which the preservation number is CGMCC No.9800 has a better treatment effect on an anaphylactic reaction caused by beta-lactoglobulin and can be used for promoting the differentiation of dendritic cells more effectively and reducing the maturity of the dendritic cells.

Description

technical field [0001] The present invention relates to the application of Lactobacillus paracasei, in particular to the application of Lactobacillus paracasei CGMCC No.9800 with anti-allergic effect in regulating the immune function of the body, and the present invention also relates to a functional food composition and its preparation method. Background technique [0002] Immunity is a physiological function of the human body, which involves non-specific immunity and specific immunity. Non-specific immunity does not require prior contact with antigens. Once pathogens enter the body, they will quickly eliminate pathogens. Both phagocytes and natural killer cells have clearance and killing functions, and are important members of the non-specific immune system. Specific immunity has immune cells (T lymphocytes and B lymphocytes) that specifically recognize foreign substances and have immune memory capabilities. The most important information transducers between the immune s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A23L1/29A61P37/02A61P37/08A61P43/00A23L33/00
Inventor 任发政杨景郭慧媛张昊赵亮刘松玲
Owner CHINA AGRI UNIV
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