Bio-synthesis method of nano zinc oxide by utilizing lactic acid bacteria and nano zinc oxide composite feed addictive

A technology of nano-zinc oxide and biosynthesis, which is applied in the field of microbial fermentation and nano-technology, can solve problems such as unseen technical reports, achieve the effects of reducing pollution, improving the utilization rate of nutrients, and preventing zinc poisoning in animals

Active Publication Date: 2014-02-26
BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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  • Bio-synthesis method of nano zinc oxide by utilizing lactic acid bacteria and nano zinc oxide composite feed addictive
  • Bio-synthesis method of nano zinc oxide by utilizing lactic acid bacteria and nano zinc oxide composite feed addictive
  • Bio-synthesis method of nano zinc oxide by utilizing lactic acid bacteria and nano zinc oxide composite feed addictive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] The preparation method of the nano-zinc oxide product biosynthesized by the highly zinc-resistant Lactobacillus salivarius L3 obtained after screening domestication from the weaned suckling pig feces in June 2011 by the inventor comprises the following steps: the high zinc-resistant Lactobacillus salivarius L3 The strain was deposited in the China Center for Type Culture Collection on April 8, 2013, and the preservation number is CCTCCM2013126.

[0049] S1. Fermentation culture of Lactobacillus. The seed medium and fermentation medium are MRS liquid medium, the pH value is 6.2-6.4, sterilized by moist heat at 121°C for 20-30min, and cooled to 37°C for later use. After activating the glycerol strains for 12 to 16 hours, insert the fermentation medium with an inoculum of 3% to 5% (V / V). After 10 hours of cultivation, add an equal volume of fresh medium to continue the cultivation for 8 hours, and the number of cells reaches 10. 9 CFU / ml, to obtain the fermentation broth;...

Embodiment 2

[0057] Utilize Lactobacillus plantarum (purchased from China Industrial Microorganism Culture Collection Management Center) to prepare nano-zinc oxide steps as follows:

[0058] S1. Fermentation and cultivation of Lactobacillus plantarum. After activating the glycerol strains for 16 to 24 hours, insert the fermentation medium with an inoculum of 3% to 5% V / V, add the same amount of fresh medium after 14 hours of cultivation and continue to cultivate for 12 hours, and the number of cells reaches 10 9 CFU / ml.

[0059] S2. Slowly add an equal amount of 0.35MZnCl to the fermentation broth while stirring 2 solution.

[0060] S3. After adjusting the pH value of the mixture to 6.0, place it in an 80° C. water bath and heat it for 25 minutes to incubate.

[0061] S4. After hatching, put them into a 37° C. incubator to age for 9 hours.

[0062] S5. After aging, the cells and nano-zinc oxide precipitates are collected by centrifugation.

[0063] S6. The centrifuged product is calci...

Embodiment 3

[0066] Utilize Lactobacillus plantarum (purchased from China Industrial Microorganism Culture Collection Management Center) to prepare nano-zinc oxide steps as follows:

[0067] S1. Fermentation and cultivation of Lactobacillus plantarum. After activating the glycerol strains for 16 to 24 hours, insert the fermentation medium with an inoculum of 3% to 5% V / V, add the same amount of fresh medium after 14 hours of cultivation and continue to cultivate for 12 hours, and the number of cells reaches 10 9 CFU / ml.

[0068] S2. Slowly add an equal amount of 0.35MZnSO to the fermentation broth while stirring 4 liquid.

[0069] S3. After adjusting the pH value of the mixture to 6.0, place it in an 80° C. water bath and heat it for 25 minutes to incubate.

[0070] S4. After hatching, put them into a 37° C. incubator to age for 9 hours.

[0071] S5. After aging, the cells and nano-zinc oxide precipitates are collected by centrifugation.

[0072] S6. The centrifuged product was dried ...

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Abstract

The invention relates to the technical fields of microorganism fermentation and nano, and provides a bio-synthesis method of nano zinc oxide by utilizing lactic acid bacteria and a nano zinc oxide composite feed addictive. The invention utilizes lactic acid bacteria to synthesize nano grade zinc oxide, and then develops a novel feed addictive which is rich in nano zinc oxide. The addictive is applied to a feed for suckling pigs, and the addition amount is less than 1000 ppm (by the weight of zinc oxide). The addictive can effectively prevent animals from zinc poisoning and reduce the environmental pollution caused by zinc, can also effectively prevent weaned pigs from diarrhea and improve the health level of intestinal tracts through the co-function of lactic acid bacteria and zinc oxide, and can even wholly or partially replace the antibiotic to reduce the antibiotic residue so as to improve the food safety.

Description

technical field [0001] The invention relates to the fields of microbial fermentation and nanotechnology, and more specifically relates to a method for biosynthesizing nano-zinc oxide by lactic acid bacteria and a nano-zinc oxide compound feed additive. Background technique [0002] Nano zinc oxide has unique properties. Nano-zinc oxide is a new type of high-function fine inorganic product, also known as ultra-fine zinc oxide, its particle size is generally less than 100nm. Due to the miniaturization of particle size and the increase of specific surface area, nano zinc oxide has surface effects, small size effects and macroscopic quantum tunneling effects that its bulk materials do not have. [0003] Nano zinc oxide can be used as a feed additive. Adding nano-zinc oxide to animal feed as a feed additive, its high biological activity and absorption rate can effectively reduce diarrhea, reduce the ratio of feed to meat, and has no pollution to the environment. It is an ideal ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/225C12R1/25C12R1/01
Inventor 胡文锋刘小阳庞旭吴俊松郑育鹏林晓慧
Owner BIOFORTE BIOTECHNOLOGY (SHENZHEN) CO LTD
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