Two deodorizing strains and application thereof in preparation of composite biological deodorant

A biological deodorant and microbial strain technology, applied in the field of microbial deodorization, can solve the problems of antagonism without considering the appropriate proportion of strains, unstable product quality and use effect, false number of effective bacteria, etc., and achieve excellent ammonium removal. Root ion capacity and growth adaptability, significant odor removal effect, sensory odor reduction effect

Active Publication Date: 2018-05-18
GUANGDONG BOWOTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biological deodorization products in the domestic and foreign markets generally have problems such as low bacterial strains and high effective bacterial counts
In addition, many products have blindly

Method used

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  • Two deodorizing strains and application thereof in preparation of composite biological deodorant
  • Two deodorizing strains and application thereof in preparation of composite biological deodorant
  • Two deodorizing strains and application thereof in preparation of composite biological deodorant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation and preservation of lactic acid bacteria:

[0031] Take capers samples, add appropriate amount of saline to grind. Dilute the grinding solution to different concentrations, take 10 -6 、10 -7 , 10 -8 Gradient bacterial solution coating CaCO 3- MRS (enrichment medium: MRS agar medium containing calcium carbonate, calcium carbonate with a mass concentration of 20 g / L added to the MRS agar medium) plate, cultured anaerobically at 37° C. for 48 hours. Pick the colonies with larger calcium-dissolving circles and separate them by streaking on the MRS plate, and purify repeatedly. The purified colonies were subjected to Gram staining, microscopic examination and H 2 o 2 For the contact enzyme test, pick out spore-free, contact enzyme-negative Gram-positive strains (R1), expand the culture and preserve the glycerol tubes and freeze-dried tubes.

[0032] 16S rDNA identification of strain R1:

[0033] The genomic DNA of strain R1 was extracted, and the PCR produc...

Embodiment 2

[0035] Isolation and preservation of yeast:

[0036] Prepare the medium used for enrichment, the formula is as follows:

[0037] Enrichment medium: 20g glucose, 20g peptone, 10g yeast extract, 100mg penicillin (to exclude bacterial interference during screening), 1000mL distilled water. Take 2g of compost sample, put it into a conical flask filled with 50mL of the above-mentioned medium, culture at 28°C, 180rpm, for 7 days for enrichment.

[0038] Isolation, purification and preservation of strain J2:

[0039] For the above-mentioned enriched culture, the solid plate (PDA medium) was coated by the dilution coating method; the colony morphology was observed with the naked eye, and purified by streaking, and the purified colony was picked for microscopic examination, and the yeast was determined by morphological characteristics. For the purified strain (J2), expand the culture and preserve the glycerol tube and freeze-dry tube.

[0040] 18S ITS rDNA identification of strain J...

Embodiment 3

[0043] Quantitative detection of ammonia removal ability of strains R1 and J2:

[0044] Single bacterium liquid culture: inoculate strain R1 into MRS medium, cultivate in an anaerobic incubator at 37°C for 36 hours; inoculate strain J2 into PDB medium, cultivate at 28°C, 180rpm for 21h. The bacterial counts of the two strains reached 1×10 8 cfu / mL.

[0045] The method for detecting the ammonia removal ability of the above strains is based on the micro-diffusion method in the national food safety standard "GB 5009.228-2016 Determination of Volatile Base Nitrogen in Food". The test material is poultry manure organic fertilizer that has not been composted. Weigh 1g of poultry manure organic fertilizer in the outer chamber of each diffusion dish, and spray 1mL of the above-mentioned bacterial strain R1 or J2 bacterial solution. Take 3mL of boric acid and add two titration nitrogen indicators dropwise, repeat each treatment 6 times, seal and incubate in a 37°C incubator, and titr...

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Abstract

The invention discloses two deodorizing strains and application thereof in preparation of a composite biological deodorant. The deodorizing strains are Lactobacillus paracasei R1 and Wickerhamomyces anomalus J2. Experiments prove that compounding of the Lactobacillus paracasei R1 and Wickerhamomyces anomalus J2 in an appropriate ratio can achieve a strong deodorization effect on feces and the like, the prepared composite biological deodorant can effectively remove odor from culturing farms, toilets and other environments or places for a long term, and has good application prospect and market value.

Description

technical field [0001] The invention belongs to the technical field of microbial deodorization. More specifically, the invention relates to two bacterial strains with deodorizing effects, and the two bacterial strains are used to prepare a composite biological deodorizer for removing odors in various environments. Background technique [0002] Odor or odor is an important environmental pollutant. In addition to affecting perception and environmental experience, it also directly endangers human health. With the rapid development of large-scale and intensive animal husbandry in my country, the production of livestock and poultry manure is increasing year by year. The relatively concentrated livestock and poultry manure has brought great harm to the health of the livestock and poultry themselves, the breeders and the residents around the farm. hazards. Freshly discharged poultry manure contains NH 3 , H 2 Harmful gases such as S and indole, if not removed in time or not proce...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16B01D53/84B01D53/58B01D53/52C12R1/225C12R1/645
CPCB01D53/52B01D53/58B01D53/84C12N1/16C12N1/20C12N1/145C12N1/205C12R2001/225C12R2001/645Y02A50/20
Inventor 陈猛李安章朱红惠姚青陈美标
Owner GUANGDONG BOWOTE BIOTECH CO LTD
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