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Method for preparing liquid complex enzyme for feeding

A liquid compound enzyme and filtrate technology, applied in the application, animal feed, animal feed and other directions, can solve problems such as increasing the cost of feed enzymes, and achieve the effects of protection effectiveness, feed quality improvement and cost reduction

Active Publication Date: 2009-01-28
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Adoption of this technology will greatly increase the cost of feed enzymes

Method used

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  • Method for preparing liquid complex enzyme for feeding
  • Method for preparing liquid complex enzyme for feeding
  • Method for preparing liquid complex enzyme for feeding

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] First, the solid fermented koji is broken, and the koji block is crushed to below 5mm particle size with a crusher, and water is added to the koji according to the weight ratio of koji: water=1:1, and the koji is stirred and moistened; then the wet koji Squeeze with a press to obtain filtrate and slag, and the slag can be used as solid feed enzyme after drying. Add its 5% weight diatomite filter aid in the squeezed filtrate, obtain the filtrate after filtering with a plate-and-frame filter; Glycerin stabilizer and potassium sorbate preservative are added into the liquid, and the liquid compound enzyme for feeding can be obtained by stirring evenly. Its enzyme components include xylanase, pectinase, mannanase, β-glucanase, cellulase, protease and other components. After determination, the activities of several enzyme components in this example are: xylanase 200000U / ml, pectinase 20000U / ml, mannanase 1000U / ml, β-glucanase 150000U / ml, fiber Sulfase 3000U / ml, protease 100...

Embodiment 2

[0026] The process is the same as in Example 1, 313kg of solid fermented koji material is crushed, and 313kg of water is added to stir. After squeezing, 315kg of filtrate and 308kg of slag are obtained; 311kg of filtrate is obtained by filtration, and 78kg of protective agent is added to obtain 389kg of liquid compound enzyme for feed. Among them, xylanase 241167u / ml, pectinase 80645u / ml.

Embodiment 3

[0028] The process is the same as in Example 1, 625kg of solid fermented koji material is crushed, and 625kg of water is added to stir. After squeezing, 630kg of filtrate and 617kg of slag are obtained; 626kg of filtrate is obtained by filtration, and 157kg of protective agent is added to obtain 783kg of liquid compound enzyme for feed. Among them, xylanase 255633u / ml pectinase 81632u / ml.

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Abstract

The invention discloses a method for preparing a liquid compound enzyme. The method comprises the steps that: strain is selected to carry out solid fermentation on materials; the solid fermentation materials are smashed and added with a certain amount of water for blending and wetting; then filtrate and dregs are obtained by pressing the mixture; after the dregs are dried, solid feed enzyme is obtained; the pressed filtrate is filtered, then antisepticise stabilizing agent is added in filter clogging liquid to obtain liquid compound enzyme used in fodder. The application of the method ensures that all enzymes in the prepared liquid compound enzyme are mutual complement, independent, has non-antagonism and the activity is not affected; moreover, the cost of the liquid compound enzyme can be reduced.

Description

Technical field [0001] The invention relates to a preparation method of a compound enzyme for feed, in particular provides a method for preparing a liquid compound enzyme for feed from a solid fermented compound enzyme koji material, and belongs to the field of biotechnology. Background technique [0002] Poultry and livestock are mostly fed in the form of powder or pellets. When using powder, the enzyme can be added to the premix, mixed well, and fed directly. If pelleted feed is used for feeding, the problem of protein denaturation will be involved when the pelleted feed is pelletized at high temperature. Enzyme is an active protein, which is sensitive to the environment like all proteins, and will be denatured and inactivated at high temperature. Before the feed enters the cooling system after pelleting, the temperature of the feed is high, up to 90°C. Such a high temperature will cause the activity of the endogenous enzymes of the feed itself and the activities of the ...

Claims

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Application Information

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IPC IPC(8): A23K1/165A23K20/189
Inventor 黄炳亮陈刚范志恒
Owner QINGDAO VLAND BIOTECH GRP
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