The present invention discloses a yacon biscuit which is prepared by the following raw materials in parts by weight: 100 parts of flour, 12-15 parts of palm oil, 5-10 parts of yacon micro powder, 8-12 parts of eggs, 15-18 parts of milk powder, 0.5-1.5 parts of baking soda, 1-2 parts of ammonium bicarbonate, 4-6 parts of red dates, 1-5 parts of Chinese wolfberries, 2-4 parts of grape seed ultrafine powder, 10-15 parts of honey, 4-6 parts of xylitol, 3-5 parts of food additives, 5-10 parts of edible salt, and an appropriate amount of water. The preparation method is simple and environment-friendly, and the prepared biscuit is not only delicious and tasty, but also has functions of promoting gastrointestinal peristalsis, promoting digestion, regulating gastrointestinal environment, preventing oxidation, lowering blood fat, blood pressure and cholesterol, tonifying liver and moistening lungs, beautifying features and preventing fatigue, etc.