Poliovirus vaccine for oral administration
A polio and virus vaccine technology, applied in the biological field, can solve the problems that affect the effectiveness and safety of vaccines, are not as good as liquid preparations for young children, vaccine storage conditions and validity period restrictions, etc. Effects of immune response stimulation capacity, effectiveness and active protection
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Embodiment 1
[0038] The preparation of embodiment 1 poliovirus antigen
[0039] Poliovirus (hereinafter referred to as poliovirus) strains sabin type I, sabin type II, and sabin type III provided by ATCC of the United States to Beijing Kexing Zhongwei Biotechnology Co., Ltd. were adapted to grow in Vero cells and serially passaged , put it in an incubator at 37.5±1.0°C for 3-5 days to harvest the virus liquid, clarify and filter it with CCID 50 The virus titer value was detected by the method. The above three types of poliovirus harvested in 10 7 -10 8 CCID 50 / ml range.
[0040] The stock solutions of various types of monovalent polioviruses were prepared for the antigenic components of vaccine preparations.
Embodiment 2
[0041] Preparation of Example 2 Poliovirus Vaccine
[0042] (1) Prepare 3M phosphate buffer solution (in which the molar concentration ratio of potassium dihydrogen phosphate and dipotassium hydrogen phosphate is 1:2) and 4M sodium succinate solution stock solution, filter and sterilize, and store at 4°C for later use;
[0043] (2) Mix 10ml of phosphate buffer prepared in step (1) with 15ml of sodium succinate solution;
[0044] (3) Add 150ml of 60% sucrose solution to (2);
[0045] (4) Add 100ml of the antigen solution in Example 1 to (3), the antigen solution is a mixture of sabin type I, type II and type III;
[0046] (5) Use virus-free MEM culture medium to supplement the volume to 300ml; the pH of the vaccine is 7;
[0047] (6) Filter and dispense, 3ml per dose, 100 doses in total.
[0048] Each dose of the vaccine prepared in this embodiment contains the following ingredients: each of the above three serotype polioviruses contains 10 7 -10 8 CCID 50 , 30% sucrose, ...
Embodiment 3
[0049] Example 3 Antacid Effect of Poliovirus Vaccine Containing Different Concentrations of Sucrose
[0050] (1) Preparation of vaccine preparations
[0051] Prepare 4 groups of vaccines containing different sucrose concentrations: Prepare 4 groups of 10ml 3M phosphate buffer solution (the molar concentration ratio of potassium dihydrogen phosphate and dipotassium hydrogen phosphate is 1:2) and 15ml 4M sodium succinate solution, and add to the 4 groups Sucrose solutions containing 30g, 90g, 150g, and 180g of sucrose were added to the mixed solution to prepare 4 kinds of protective agents containing different concentrations of sucrose (phosphate solution + sodium succinate solution + sucrose solution), and added to the 4 groups of protective agents The 100ml antigenic component in Example 1 (same as Example 2, mixed with 3 serotypes) was filled up to 300ml with virus-free MEM culture solution, filtered and sterilized, and divided into 3ml per dose to prepare 100 doses of vacc...
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