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Profitable soil microorganism mixed fermentation method

A mixed fermentation and microbial technology, which is applied in the field of mixed fermentation of Issaccharomyces orientalis, Bacillus licheniformis, and soil beneficial strain Paecilomyces lilacinus, can solve the problems that the fermentation process has no special instructions, and achieve simple and efficient fermentation methods and formulas Simple, reduce the effect of chemical fertilizer and pesticide application

Active Publication Date: 2016-02-10
FUJIAN SANJU BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 201410617387.5 discloses a microbial powder for growing rice and corn. The added beneficial microorganisms involve Bacillus licheniformis and yeast, but there is no special description for the fermentation process

Method used

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  • Profitable soil microorganism mixed fermentation method
  • Profitable soil microorganism mixed fermentation method
  • Profitable soil microorganism mixed fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Mixed medium formulation optimization

[0029]With beef extract 3g / L, peptone 12g / L, glucose 20g / L, potassium dihydrogen phosphate 2g / L, and zinc sulfate 0.1g / L as the basal medium, the culture conditions were initial pH 6.5, 28°C, 180rpm shaking 3d. Three strains of bacteria could be observed through microscopic examination and medium plate coating, figure 1 It is the colony morphology of the three strains of bacteria on the basal medium, indicating that the basal medium is suitable for the cultivation of the three strains of bacteria.

[0030] Substituting sucrose, maltose, and soluble starch for glucose in the basal medium with the addition of 20g / L respectively, investigated their influence on the effective number of viable bacteria of the three strains of mixed culture, as figure 2 As shown, when soluble starch was used as carbon source, the total effective number of viable bacteria of the three strains was the highest, followed by sucrose, maltose and...

Embodiment 2

[0041] The culture effect comparison of embodiment 2 bacterial strain specific medium and mixed fermentation medium

[0042] After the activation of Issaccharomyces orientalis, scrape a ring of bacteria from the slope and put it in a triangular flask filled with normal saline, shake it well and dilute to OD 600 0.2, with 10% of the inoculum amount, inoculated respectively in the following three liquid culture mediums known to cultivate Issahia orientalis and the mixed fermentation liquid medium of the present invention, cultivated at 28°C and 180rpm for 48h, and took samples to detect effective viable bacteria number.

[0043] The substratum of three kinds of known oriental Issa yeasts of table 3 and the formula of mixed fermentation medium of the present invention

[0044]

[0045] After activation of Bacillus licheniformis, scrape a ring of bacteria from the inclined plane and put it in a triangular flask filled with normal saline, shake well and dilute to OD 600 0.2, i...

Embodiment 3

[0052] The quality control of embodiment 3 mixed fermentation inoculum size

[0053] In order to control the effective number of viable bacteria in the initial inoculation of various bacteria in the mixed fermentation system, so that the initial effective number of viable bacteria of various bacteria is within the known controllable range, the effective number of viable bacteria in the seed solution and the OD 600 The relationship between the values, the number of effective viable bacteria and OD of the three bacterial seed solutions were made 600 Standard curve between values.

[0054] After inoculation of Paecilomyces lilacinus, culture 4-6 days to collect spores, wash the spores with normal saline to prepare spore suspension, adjust the spore suspension to OD with sterile water 600 was 0.2, inoculated the spore suspension into the liquid medium with 10% inoculum, cultivated it at 28°C and 180rpm for 2 days, and prepared Paecilomyces lilacinus seed liquid, which was counted...

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Abstract

The invention discloses a profitable soil microorganism mixed fermentation method. The method includes the steps of 1) activating paecilomyces lilacinus, issatchenkia orientalis and bacillus licheniformis on a PDA (potato dextrose agar) medium, a YPD (yeast extract peptone dextrose) medium and a nutrient agar medium respectively, and transferring the three activated strains to corresponding liquid media so as to prepare seed fermentation broth; 2) inoculating 10% of each of the three strains into a mixed fermentation medium, and controlling the living bacteria count of the bacillus licheniformis to be one order of magnitude higher than those of the paecilomyces lilacinus and the issatchenkia orientalis according to the relation between the living bacteria count of the seed fermentation broth and an OD600 (optical density 600) value. The profitable soil microorganism mixed fermentation method has the advantages that a mixed fermentation medium formula is simple, production cost is reduced, equipment utilization rate is increased, and accordingly the profitable soil microorganism mixed fermentation method is suitable for large-scale production of fermented products; the fermented products are capable of improving soil flora balance, promoting growth and increasing production and can be used for producing biofertilizer and biopesticide by being compounded with organic materials, soil improvement agents and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a method for mixed fermentation of beneficial soil bacteria strains Paecilomyces lilacinus, Issa orientalis and Bacillus licheniformis. Background technique [0002] With the development of agricultural intensification, policy support, and people's demands for green and organic products, modern agriculture has also encountered many thorny problems, such as soil compaction, serious soil-borne diseases, continuous cropping obstacles, and crop quality decline. A large amount of chemical fertilizers and pesticides are applied to the soil, which affects the ecological environment and human health, and microbial products are the only way to solve these problems, especially in the context of human beings facing energy crises, resource shortages, and environmental pollution (Li Jun, 2006). Microbial products are irreplaceable in improving soil fertility, improvi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C05G3/04A01N63/04A01N63/02A01P21/00C12R1/10C12R1/645C12R1/79C05G3/80
Inventor 余劲聪林克明
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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