Profitable soil microorganism mixed fermentation method

A mixed fermentation and microbial technology, which is applied in the field of mixed fermentation of Issaccharomyces orientalis, Bacillus licheniformis, and soil beneficial strain Paecilomyces lilacinus, can solve the problems that the fermentation process has no special instructions, and achieve simple and efficient fermentation methods and formulas Simple, reduce the effect of chemical fertilizer and pesticide application

Active Publication Date: 2016-02-10
FUJIAN SANJU BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Chinese patent 201410617387.5 discloses a microbial powder for growing rice and corn. The added beneficial microor

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  • Profitable soil microorganism mixed fermentation method
  • Profitable soil microorganism mixed fermentation method
  • Profitable soil microorganism mixed fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1 Optimization of mixed medium formula

[0029] Take beef extract 3g / L, peptone 12g / L, glucose 20g / L, potassium dihydrogen phosphate 2g / L, zinc sulfate 0.1g / L as the basic medium, and the culture conditions are starting pH 6.5, 28°C, 180rpm shaking 3d. Three strains can be observed through microscopic examination and medium plate coating, figure 1 The colony morphology of the three bacteria on the basic medium indicates that the basic medium is suitable for the cultivation of three bacteria.

[0030] Sucrose, maltose, and soluble starch were used to replace the glucose in the basal medium with the addition amount of 20g / L respectively, to investigate their influence on the effective viable count of the three strains of mixed culture, such as figure 2 As shown, when soluble starch is used as the carbon source, the total number of effective viable bacteria of the three strains is the highest, followed by sucrose, maltose, and glucose. The effect of soluble starch, su...

Example Embodiment

[0041] Example 2 Comparison of the culturing effect of strain specific medium and mixed fermentation medium

[0042] After the activation of Isa orientalis, scrape a ring of bacteria from the inclined surface into a triangular flask filled with physiological saline, shake it and dilute to OD 600 Inoculate the following three liquid mediums known to be capable of cultivating Isa orientalis and the mixed fermentation liquid medium of the present invention at an inoculum amount of 10%. Cultivate for 48 hours at 28°C and 180 rpm, and sample to detect effective live bacteria number.

[0043] Table 3 Three kinds of known Isa yeast culture media and the mixed fermentation medium formula of the present invention

[0044]

[0045] After the activation of Bacillus licheniformis, scrape a loop of bacteria from the inclined surface and place it in an Erlenmeyer flask filled with saline, shake it and dilute to OD 600 It is 0.2, inoculated into the following three known liquid mediums capable of c...

Example Embodiment

[0052] Example 3 Quality control of mixed fermentation inoculum

[0053] In order to control the effective viable number of the initial inoculation of various bacteria in the mixed fermentation system, make the initial effective viable number of various bacteria in the known and controllable range, combining the effective viable number of seed liquid and OD 600 The relationship between the values, the number of effective viable bacteria and the OD 600 Standard curve between values.

[0054] After inoculation of Paecilomyces lilacinus, culture for 4-6 days to collect spores, wash the spores with saline to prepare a spore suspension, adjust the spore suspension to OD with sterile water 600 The spore suspension was inoculated into liquid culture medium with 10% inoculum amount, cultured at 28℃ and 180rpm for 2 days to prepare Paecilomyces lilacinus seed solution. After dilution, plate count and OD 600 Value measurement, the effective viable count (100 million / mL) and OD of Paecilomyces ...

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Abstract

The invention discloses a profitable soil microorganism mixed fermentation method. The method includes the steps of 1) activating paecilomyces lilacinus, issatchenkia orientalis and bacillus licheniformis on a PDA (potato dextrose agar) medium, a YPD (yeast extract peptone dextrose) medium and a nutrient agar medium respectively, and transferring the three activated strains to corresponding liquid media so as to prepare seed fermentation broth; 2) inoculating 10% of each of the three strains into a mixed fermentation medium, and controlling the living bacteria count of the bacillus licheniformis to be one order of magnitude higher than those of the paecilomyces lilacinus and the issatchenkia orientalis according to the relation between the living bacteria count of the seed fermentation broth and an OD600 (optical density 600) value. The profitable soil microorganism mixed fermentation method has the advantages that a mixed fermentation medium formula is simple, production cost is reduced, equipment utilization rate is increased, and accordingly the profitable soil microorganism mixed fermentation method is suitable for large-scale production of fermented products; the fermented products are capable of improving soil flora balance, promoting growth and increasing production and can be used for producing biofertilizer and biopesticide by being compounded with organic materials, soil improvement agents and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a method for mixed fermentation of beneficial soil bacteria strains Paecilomyces lilacinus, Issa orientalis and Bacillus licheniformis. Background technique [0002] With the development of agricultural intensification, policy support, and people's demands for green and organic products, modern agriculture has also encountered many thorny problems, such as soil compaction, serious soil-borne diseases, continuous cropping obstacles, and crop quality decline. A large amount of chemical fertilizers and pesticides are applied to the soil, which affects the ecological environment and human health, and microbial products are the only way to solve these problems, especially in the context of human beings facing energy crises, resource shortages, and environmental pollution (Li Jun, 2006). Microbial products are irreplaceable in improving soil fertility, improvi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N1/14C05F11/08C05G3/04A01N63/04A01N63/02A01P21/00C12R1/10C12R1/645C12R1/79C05G3/80
Inventor 余劲聪林克明
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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