Probiotics yoghourt powder and preparation method thereof
A technology of probiotic acid and probiotics, which is applied in the field of yogurt powder preparation, can solve the problems of ignoring the pure nature of yogurt, product biological effect discount, high shear force, etc.
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Embodiment 1
[0043] The 4 strains of probiotics used in the present invention are all purchased from the China Industrial Microorganism Culture Preservation Management Center, which are: Lactobacillus delbrueckii subsp. In the present invention, the above bacterial strains are referred to as: A bacteria, B bacteria, C bacteria, D bacteria.
[0044] 1. Selection of probiotics and preparation of medium
[0045] Bacteria A—Lactobacillus delbrueckii subsp.bulgaricus CICC6047 (Lactobacillus delbrueckii subsp.bulgaricus), culture medium: 8% by mass skim milk powder solution, sterilized at 121°C for 20 minutes and cooled to 30°C for later use.
[0046] Bacteria—Lactobacillus rhamnosus CICC22173 (Lactobacillus rhamnosus), culture medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, Tween80 1.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O 0.05g, CaCO 3 20.0g, 1.0L distilled water, pH 6.8, sterilized at 121°C fo...
Embodiment 2
[0077] 1. Selection of probiotics and preparation of medium
[0078] Bacteria A—Lactobacillus delbrueckii subsp.bulgaricus CICC6047 (Lactobacillus delbrueckii subsp.bulgaricus), culture medium: 8% by mass skim milk powder solution, sterilized at 121°C for 20 minutes and cooled to 30°C for later use.
[0079] Bacteria—Lactobacillus rhamnosus CICC22173 (Lactobacillus rhamnosus), culture medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, Tween80 1.0g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O 0.05g, CaCO 3 20.0g, 1.0L distilled water, pH 6.8, sterilized at 121°C for 20 minutes and cooled to 30°C for later use.
[0080] Bacteria C—Lactobacillus paracasei CICC6230 (Lactobacillus paracasei) medium: 10.0g casein peptone, 10.0g beef extract, 5.0g yeast powder, 5.0g glucose, 5.0g sodium acetate, 2.0g diamine citrate, 1.0 Tween80 g, K 2 HPO 4 2.0g, MgSO 4 ·7H 2 O 0.2g, MnSO 4 ·H 2 O 0.05...
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