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Method for brewing grape vinegar by using fresh grape spirit residual liquid

A technology of table grapes and distilled wine, which is applied in the field of vinegar-making technology, can solve the problems of large differences in fermentation capacity of vinegar-making raw materials, lack of specificity of acetic acid bacteria, differences in acetic acid content and taste, and achieve mellow and mellow vinegar body. Long shelf life and strong flavor effects

Inactive Publication Date: 2018-07-24
四川缪氏现代农业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different types and sources of acetic acid bacteria have great differences in their ability to ferment vinegar raw materials, and the content and taste of acetic acid produced also vary greatly.
The current strains used for the production of fruit vinegar generally use acetic acid bacteria for brewing grain vinegar. The acetic acid strains used for production lack specificity, resulting in the lack of obvious fruit aroma and flavor that fruit vinegar should have.

Method used

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  • Method for brewing grape vinegar by using fresh grape spirit residual liquid
  • Method for brewing grape vinegar by using fresh grape spirit residual liquid
  • Method for brewing grape vinegar by using fresh grape spirit residual liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 obtains raffinate of grape distilled wine

[0027] 1. Isolation and purification of grape wild yeast

[0028] After the fresh grape fruit is broken, it is multiplied and cultured at 29-31° C. for 48 hours to obtain a grape wild yeast proliferation culture solution with more individuals. The obtained proliferation culture solution was diluted with sterile distilled water according to seven concentration gradients of 10 times, 20 times, 40 times, 80 times, 160 times, 320 times and 640 times, and then spread on the plate selection medium respectively (plate selection Medium formula: 4-5°Bé fresh grape pulp filtrate 24.5%, 4°Bé wort juice 24.5%, 4°Bé soybean juice 24.5%, 4°Bé potato juice 24.5%, agar 2%), each dilution concentration The gradient culture solution was used to make 4 plates, and dozens of single-cell colonies of grape wild yeast were isolated by "plate streaking method". According to the colony shape, single-cell individual shape, and growth of ...

Embodiment 2

[0068] Embodiment 2 Utilizes grape distilled wine residue to brew grape vinegar

[0069] 1. Isolation and purification of grape wild acetic acid bacteria

[0070] The fresh grape fruit is crushed and multiplied and cultured for 48 hours under the condition of 30-33° C. to obtain a multiplication culture solution of grape wild acetic acid bacteria with many individuals. The obtained proliferation culture solution was diluted with sterile distilled water according to seven concentration gradients of 10 times, 20 times, 40 times, 80 times, 160 times, 320 times and 640 times, and then spread on the plate selection medium respectively (plate selection medium) Culture medium formula: 3-4Bé grape juice 90 ml, yeast extract 1% calcium carbonate 2%, 65% alcohol 10 ml, agar 2.2%), each concentration gradient of the culture solution is made 4 plates, using the "plate streaking method" "Isolated dozens of single-cell colonies of Acetobacter viticum.

[0071] According to the colony shap...

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PUM

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Abstract

The invention discloses a method for brewing grape vinegar by using fresh grape spirit residual liquid. The method comprises the following steps: (1) preparing the grape spirit residual liquid: A, removing kernels and stalks from grape fruits, and then pulping the grape fruits to obtain grape pulp; B, inoculating grape wine yeast into the grape pulp in the step A, uniformly stirring the grape wineyeast and the grape pulp for alcohol fermentation to obtain fermented liquid; C, filtering the fermented liquid, distilling the obtained fermented filtrate to obtain distilled spirit, and taking theresidual liquid obtained by distillation as a raw material for brewing the grape vinegar; (2) inoculating grape oxidization acetic bacteria seed suspension which is 4 to 6 percent of the weight of thegrape spirit residual liquid, and uniformly stirring the grape spirit residual liquid and the seed suspension, wherein grape oxidization acetic bacteria are Miao 1.1610 with a collection number of CGMCC NO. 14778; (3) placing the inoculated grape spirit residual liquid at 31 to 33 DEG C, and feeding oxygen for fermentation for about 20 to 25 days to obtain fermented liquid; (4) disinfecting, clarifying and filtering the fermented liquid to obtain the grape vinegar.

Description

technical field [0001] The invention relates to a method for preparing fruit vinegar, in particular to a method for brewing grape vinegar by using the leftover liquid of distilled wine from table grapes, and belongs to the field of vinegar brewing technology. Background technique [0002] In the production of fruit distilled wine, the residual liquid after distillation is large and rich in nutrients. Direct discharge will inevitably seriously pollute the environment. The cost of harmless treatment is extremely high and the effect is not ideal. This is an objective problem in the industry. The large-scale pollution of water and soil caused by it not only seriously restricts the development of the industry, but also directly puts pressure on the environment. [0003] In the vinegar industry, alcohol dehydrogenase secreted by acetic acid bacteria oxidizes ethanol to acetaldehyde in an aerobic environment, and then oxidizes acetaldehyde to acetic acid (acetic acid). However, ac...

Claims

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Application Information

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IPC IPC(8): C12J1/02C12G1/022C12R1/865C12R1/02
CPCC12G1/0203C12J1/02
Inventor 杨廷元缪敏喻言田怀宇黄靖淞
Owner 四川缪氏现代农业发展有限公司
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