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Anti-plasmodiophoa-brassicae-woron inbred line breeding method

A cabbage clubroot disease and anti clubroot disease technology, which is applied in the directions of botanical equipment and methods, biochemical equipment and methods, and microbial assay/inspection, etc. Resistance to clubroot and other problems such as clubroot and Chinese cabbage, to achieve the effect of saving space and high breeding efficiency

Active Publication Date: 2017-03-15
LIAONING ACAD OF AGRI SCI
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Problems solved by technology

[0006] It can be seen from the upper right that conventional breeding methods take a long time to select materials, and it is slow to obtain pure lines. At present, there is still a lack of excellent clubroot-resistant Chinese cabbage inbred lines and excellent clubroot-resistant Chinese cabbage varieties.

Method used

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  • Anti-plasmodiophoa-brassicae-woron inbred line breeding method

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Embodiment

[0019] In the present invention, firstly, a hybrid species resistant to clubroot of Chinese cabbage is searched for, and then self-crossed to obtain F 2 Generation seeds, and then start the following breeding work:

[0020] S1. Seed soaking and germination: Put the seeds to be treated into a petri dish with absorbent cotton and filter paper in the first ten days of January, soak the seeds in water at about 30°C for 2-3 hours, and then germinate them at room temperature (about 30°C) for 12-3 hours. After 24 hours, the radicles of most of the seeds break through the seed coat, and then put them into the refrigerator to start vernalization.

[0021] S2. Vernalization treatment: control the temperature of the refrigerator at 0-3°C, rinse with water every 2 days, and perform vernalization treatment for 20-25 days. The radicle length of the vernalized seeds is generally 1 to 1.5 cm, and most of the seed coats have fallen off.

[0022] S3. Inoculation identification: (1) Bacterial ...

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Abstract

The invention discloses an anti-plasmodiophoa-brassicae-woron inbred line breeding method which comprises the steps of seed soaking for germination hastening, vernalization, inoculation and identification, and inbred pollination. The method specifically comprises the following steps: in spring, carrying out carrying out seed soaking for germination hastening and vernalization on an F2-generation anti-plasmodiophora-brassicae breeding material, carrying out inoculationand identification by a bacterium soil process, permanently growing a disease-resistant individual plant into a flowerpot, and carrying out normal culture and management; after the plant bolts and blooms, carrying out inbred pollination, and collecting the seeds; and in next spring, continuing seed soaking for germination hastening, vernalization, inoculation and identification and inbred pollination, and after 3-5 generations of selection, breeding the anti-plasmodiophoa-brassicae Chinese cabbage inbred line with regular characters. The method can enhance the selection efficiency and accelerate the breeding progress.

Description

technical field [0001] The invention belongs to the technical field of heterosis utilization of Chinese cabbage, and in particular relates to a method for cultivating clubroot-resistant inbred lines of Chinese cabbage. Background technique [0002] Chinese cabbage originates in my country and is one of the vegetable crops loved by the people. With the improvement of people's living standards, the people have put forward higher requirements for the quality, resistance and growth period of Chinese cabbage; at the same time, climate change and the development of diseases have raised many new problems for the scientific research and production of Chinese cabbage. . Especially in recent years, clubroot of Chinese cabbage has become more and more serious, which has become an important factor limiting the improvement of Chinese cabbage yield and quality. Chinese cabbage clubroot is an important disease of Brassicaceae crops caused by Plasmodiophora brassicae. The disease is a so...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04C12Q1/18
CPCA01H1/02A01H1/04C12Q1/18
Inventor 王鑫吴海东王丽丽何明田云蒋欣陶李颖杨晓飞
Owner LIAONING ACAD OF AGRI SCI
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