Method for inhibiting growth of plasmodiophora brassicae woronin, method for preventing and treating club roots of cruciferae crops and application of azalomycin

A technology of Brassicaceae crops, Plasmodium brassicae, applied to the prevention and treatment of clubroot of Brassicaceae crops, azamycin can solve the threat to the production of Brassicaceae crops, Plant chlorosis, no yield and no harvest and other problems

Active Publication Date: 2017-05-31
INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of light damage, the appearance of the aboveground tissue has no obvious symptoms; in the case of severe damage, nutrient absorption and water transportation are interrupted, the upper part shows malnutrition and wilting, and the plants show different degrees of chlorosis; for young seedlings, the upper leaves are withered, resulting in It dies immaturely, and its growth and development are seriously hinder...

Method used

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  • Method for inhibiting growth of plasmodiophora brassicae woronin, method for preventing and treating club roots of cruciferae crops and application of azalomycin
  • Method for inhibiting growth of plasmodiophora brassicae woronin, method for preventing and treating club roots of cruciferae crops and application of azalomycin
  • Method for inhibiting growth of plasmodiophora brassicae woronin, method for preventing and treating club roots of cruciferae crops and application of azalomycin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This example is used to illustrate the effect of using azamycin F3 to inhibit the growth of Plasmodium brassicae.

[0032] 1. Collection of Plasmodium brassicae

[0033] The diseased roots of cabbage, Chinese cabbage, cauliflower and rape that had clubroot were collected and planted in Jietou Town, Tengchong County, Yunnan Province, respectively, and the diseased root tissue sections were observed under a microscope. As a result, dormant sporangia and swimming sporangia were observed under the microscope. Resting spores were identified as Plasmodium brassicae.

[0034] Wash the collected fresh diseased roots and dry the surface moisture for later use. Squeeze 1g of the diseased root, try to avoid damaging the root tissue, release the spores and mix them with 50mL of sterile distilled water, filter through 15 layers of gauze, filter the filtrate through medium-speed filter paper again, put it in a 50mL centrifuge tube, and set it at 4500rpm Centrifuge for 10 min, disca...

Embodiment 2

[0042] This example is used to illustrate the effect of using azamycin F4 to inhibit the growth of Plasmodium brassicae.

[0043] 1. Collection of Plasmodium brassicae

[0044] With embodiment 1.

[0045] 2. The effect of azamycin F4 on the spores of Plasmodium brassicae

[0046] Follow the steps of Example 1, wherein the difference is that azamycin F4 is used instead of azamycin F3.

[0047] The results are shown in Table 2. The MIC values ​​of azamycin F4 against Plasmodium brassicae were all less than or equal to 2.5 μg / mL, indicating that azamycin F4 had a strong inhibitory effect on Plasmodium brassicae.

[0048] Table 2

[0049] source of spores Acting substance MIC value (μg / mL) kale Azamycin F4 1.0 kale sterile distilled water — Chinese cabbage Azamycin F4 2.5 Chinese cabbage sterile distilled water — brocoli Azamycin F4 1.5 brocoli sterile distilled water — rape Azamycin F4 2.0 rape sterile...

Embodiment 3

[0052] This example is used to illustrate the determination of the control effect of azamycin F3 on cabbage clubroot.

[0053] Choose healthy Chinese cabbage with consistent growth in the 8-9 leaf stage to measure the preventive and therapeutic effects.

[0054] 1. Preventive effect

[0055] Root irrigation with azamycin F3 (root irrigation at a concentration of 200 μg / mL), after 24 hours, the concentration of root irrigation inoculation was 1×10 5 Each / mL of the spore suspension of Plasmodium brassicae from Chinese cabbage prepared in Example 1, set sterile distilled water as the blank control, and the root irrigation volume of the three is 50mL / strain. After inoculation, they were placed in a 28°C incubator for moisturizing culture. Apply every 7 days.

[0056] 2. Therapeutic effect

[0057] The root irrigation inoculation concentration was 1×10 5 The spore suspension of Plasmodium brassicae from Chinese cabbage prepared in Example 1 was prepared in Example 1. Sterile d...

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Abstract

The invention relates to a method for inhibiting growth of plasmodiophora brassicae woronin, a method for preventing and treating club roots of cruciferae crops and application of azalomycin. Specifically, the invention provides the method for inhibiting the growth of the plasmodiophora brassicae woronin; the method comprises the steps of enabling the azalomycin to be in contact with the plasmodiophora brassicae woronin. The invention further provides the method for preventing and treating the club roots of the cruciferae crops and the method comprises the step of applying the azalomycin to the cruciferae crops. The invention further provides the application of the azalomycin to prevention and treatment of the club roots of the cruciferae crops. According to the method for inhibiting the growth of the plasmodiophora brassicae woronin, the method for preventing and treating the club roots of the cruciferae crops and the application of the azalomycin, the club roots of the cruciferae crops can be effectively prevented and treated; the azalomycin can be obtained through a microorganism manner so that the azalomycin has the advantage of safety to environment and the application prospect is wide.

Description

technical field [0001] The present invention relates to the field of clubroot prevention and control, in particular to a method for inhibiting the growth of Brassicaceae clubroot, a method for preventing and treating clubroot of cruciferous crops, and the use of azamycin in preventing and treating clubroot of cruciferous crops the use of. Background technique [0002] Clubroot of cruciferous crops is caused by Plasmodiophora brassicae Woronin infection. The disease was first reported in the United States in 1852, and it was widespread in the former Soviet Union in the 19th century and caused devastating disasters. In recent years, the disease has become increasingly serious worldwide. my country has reported it in Taiwan as early as 1936, and it is mainly distributed in Heilongjiang, Liaoning, Shandong, Xinjiang, Gansu, Shaanxi, Jiangsu, Zhejiang, Fujian, Jiangxi, Hubei, Hunan and other regions at present. After the plant is infected by Plasmodium brassicae, some or all o...

Claims

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Application Information

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IPC IPC(8): A01N47/44A01P3/00
CPCA01N47/44
Inventor 杨佩文李铭刚白亭亭杨明英曾莉番华彩杨群辉李家瑞郭志祥尚慧毕云青
Owner INST OF AGRI ENVIRONMENT & RESOURCES YUNNAN ACAD OF AGRI SCI
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