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Streptomyces cuspidosporus and application thereof in prevention and treatment of plasmodiophora brassicae

A technology of Streptomyces oxysporum, cruciferous family, applied in the application, bacteria, fungicides and other directions, can solve the problem of Streptomyces sp.

Active Publication Date: 2014-07-09
HUBEI BIOPESTICIDE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there is no Streptomyces pesticide registered for the control of cruciferous clubroot

Method used

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  • Streptomyces cuspidosporus and application thereof in prevention and treatment of plasmodiophora brassicae
  • Streptomyces cuspidosporus and application thereof in prevention and treatment of plasmodiophora brassicae
  • Streptomyces cuspidosporus and application thereof in prevention and treatment of plasmodiophora brassicae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Isolation and screening of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 strains

[0052] Soil samples were taken and separated, purified and preserved by conventional methods. We isolated 30,000 strains of actinomycetes from the soil. Using the method of lead compound activity tracking, combined with the HPLC-MS natural product compound database, we selected 109 strains of actinomycetes with relatively low toxicity, easy cultivation and industrialization tendency, and tested them through Chinese cabbage root. See table 1 for the pot experiment and field experiment of swollen disease. Finally, it was determined that WS-24926 and WS-29246 were the best, with the smallest disease index, and the corrected control effects were 95% and 79%, respectively.

[0053] Pot test implementation steps: After activating each test strain, adopt the secondary shake flask fermentation method, the primary shake flask fermentation uses the seed medium, th...

Embodiment 2

[0069] Example 2 Identification of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246

[0070] After morphological observation, physiological and biochemical experiments and molecular biological identification of the screened strains, it was finally determined that WS-24926 was Streptomyces scabella and WS-29246 was Streptomyces oxysporum.

[0071] The colony morphology of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 strains on the ISP-2 plate of the present invention are shown in the attached figure 1 , figure 2 .

[0072] 16S rDNA sequence analysis: Genomic DNA of Streptomyces scabiei WS-24926 and Streptomyces cuspidosporus WS-29246 were extracted and amplified by PCR in a 50 μL system. Using primers:

[0073] PrimerA: 5'-AGAGTTTGATCCTGGCTCAG-3';

[0074] Primer B: 5'-TACGGCTACCTTGTTACGACTT-3'.

[0075] Reaction conditions: 35 cycles of pre-denaturation at 94°C for 5 min, 1 min at 94°C, 1 min at 55.5°C, 3 min at 72°C, and ext...

Embodiment 3

[0078] Get 4kg of xanthan gum and add 112kg of water to quickly shear and mix thoroughly, then add 800kg of the standby fermented bacteria liquid (take each of the three standby fermented bacteria liquids once, and three kinds of Streptomyces can be prepared in this embodiment Bio-control suspending agent), 4kg sodium lignosulfonate, 20kg alkylbenzenesulfonate, 30kg sorbate, 20kg potassium sorbate, 10kg propylene glycol, to obtain the mixed material, stir and disperse evenly, pass through a horizontal sand mill After the secondary grinding, the particle size test shows that more than 90% of the material has a fineness of less than 5 μm, stop grinding and stir evenly, and pack it into a finished product, that is, the biocontrol suspending agent is obtained.

[0079] For clubroot of alpine off-season Chinese cabbage, the root irrigation method was adopted, with 100 mL of root irrigation at the time of transplanting, then once every 10 days, and three times during the entire growt...

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Abstract

The invention discloses streptomyces cuspidosporus and an application thereof in prevention and treatment of plasmodiophora brassicae. The preservation number of the streptomyces cuspidosporus WS-29246 is CCTCC (China Center For Type Culture Collection) NO: M2012413. The streptomyces cuspidosporus fermented generates a fermented liquid with a strong bacteriostatic action. The fermented liquid can be used for preparing a biocontrol suspension and a biocontrol slow release capsule for resisting plasmodiophora brassicae for biologically preventing and treating plasmodiophora brassicae.

Description

[0001] The present invention is a divisional application of the Chinese invention patent "Streptomyces and its application in the prevention and treatment of cruciferous clubroot" with application number 2013100497448. technical field [0002] The invention relates to the technical field of biological pesticides, in particular to Streptomyces oxysporum and its application in preventing and treating clubroot of Cruciferae. Background technique [0003] Cruciferous clubroot (plasmodiophoa brassicae Woron) is a worldwide soil-borne disease. The pathogen is Plasmodiophoa brassicae, which belongs to an obligate parasitic lower flagellate (some scholars also classify it as slime mold) , which cannot be cultured artificially. The main hazards are cruciferous vegetables such as: Chinese cabbage, cabbage, radish, etc. The pathogen survives winter and summer in the soil or undecomposed manure with sporangia. It has strong resistance to adverse environments and can generally survive f...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N25/04A01N25/28A01P3/00C12R1/465
Inventor 龙同万中义王开梅杨自文曹春霞张志刚张亚妮闵勇廖先清方伟江爱兵朱志刚姚经武张遵霞
Owner HUBEI BIOPESTICIDE ENG RES CENT
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