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Immobilized penicillin amidase carrier and its preparing method

A technology of penicillin acylase and immobilized carrier, which is applied in the direction of fixing on/in organic carrier, which can solve the problems of complex preparation of raw materials and difficulty in obtaining preparation costs, and achieve cheap and easy-to-obtain raw materials, apparent enzyme The effect of high activity and easy industrial production

Inactive Publication Date: 2003-04-09
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved in the present invention is to disclose a penicillin acylase immobilized carrier and its preparation method, so as to overcome the defects of complex preparation of raw materials, difficulty in obtaining and high preparation cost in the prior art, so as to meet the needs of relevant people

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] After mixing 870 milliliters of n-heptane with 280 milliliters of tetrachlorethylene, inject it into a four-necked flask equipped with a thermometer, airway and stirrer, add 0.4 grams of surfactant Span-60 and 50 grams of calcium stearate Heat to 55°C. After mixing evenly, add 160 milliliters of formaldehyde with 32 grams of N, N'-methylenebisacrylamide, 10 grams of acrylamide, 20 grams of glycidyl methacrylate and 0.5 grams of initiator azobisisobutyronitrile. Amide solution. Pure nitrogen was passed through, and the stirring was stopped after 6 hours of polymerization. The product was filtered, washed with acetone, extracted, and then vacuum-dried to constant weight. The apparent enzyme activity of penicillin G potassium salt hydrolysis to prepare 6-APA by immobilized penicillin acylase (IME) on this carrier was measured by p-dimethylaminobenzaldehyde (PDAB) method, which was 110 IU / g.

Embodiment 2

[0024] The difference from Example 1 is that a water separator is installed on the four-necked flask, and the diluent in the monomer phase is a mixed solution of methanol and water instead of formamide. 112 grams of methanol and 40 grams of water were added to the polymerization system, and the amounts and preparation conditions of the remaining components were the same as in Example 1. The corresponding apparent enzyme activity of the immobilized penicillin acylase was measured to be 125IU / g.

Embodiment 3

[0026] The reaction device is the same as in Example 2. Add 850 ml of cyclohexane into a four-necked bottle, add 0.6 g of Span-60 and 32 g of calcium stearate, and heat to 55°C. After the Span-60 dissolution system reaches uniform mixing, add 156 ml of 60wt% A mixture solution of 28 grams of N,N'-methylene bisacrylamide, 20 grams of glycidyl methacrylate and 0.5 grams of azobisisobutyronitrile dissolved in methanol aqueous solution was uniformly dispersed and then passed into nitrogen. After 6 hours of polymerization, most of the water enters the water separator and is separated from the system, and the reaction is stopped at this time. The aftertreatment of product is with embodiment 1. The corresponding apparent enzyme activity of immobilized penicillin acylase was measured to be 92IU / g.

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Abstract

The present invention discloses a kind of immobilized penicillin amidase carrier and its preparation process. The immobilized pencillin amidase carrier is prepared with surfactant as dispersant and vinyl compound as monomer and through crosslinking polymerization in reverse suspension technology. The carrier immobilized pencillin amidase has an apparat enzyme activity in preparing 6-APA with benzyl pencillin potassium as high as 125 IU / g. The polymer bead carrier containing epoxy group of the present invention has high apparent enzyme activity, simple technological process, easy operation andcheap material and may be used in industrial production.

Description

technical field [0001] The present invention relates to an enzyme immobilization carrier, in particular to a penicillin acylase immobilization carrier, more specifically to a preparation method for a penicillin acylase immobilization carrier of a bead cross-linked polymer containing epoxy groups . Background technique [0002] β-lactam antibiotics, such as semi-synthetic penicillin and semi-synthetic cephalosporin, are currently widely used antibiotics. The traditional semi-synthetic chemical method has many steps, complicated process, low yield and serious pollution. The immobilized penicillin acylase is used for the semi-synthetic modification of antibiotics. Due to the high operational stability of the immobilized enzyme, the production process is easy to achieve continuous operation and controllability. After the reaction, it is easy to separate from the product and can be used repeatedly. Because of the high quality and low production cost of the obtained product, it ...

Claims

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Application Information

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IPC IPC(8): C08F2/18C08F20/00C12N11/08
Inventor 卢冠忠薛屏乌云高娃郭杨龙王筠松
Owner EAST CHINA UNIV OF SCI & TECH
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