Magnetic polymer microsphere for enzyme immobilization and preparation method thereof

A polymer, biological enzyme technology, applied in the direction of the magnetic properties of organic materials/organic magnetic materials fixed on/in organic carriers, can solve the problems of difficult recovery of biological enzymes, affecting product performance, instability, etc. The effect of reducing time and solvent consumption, improving mechanical strength and prolonging service life

Inactive Publication Date: 2008-08-27
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] There are many shortcomings in the direct use of biological enzymes as catalysts: it is unstable in high temperature, strong acid, strong alkali and organic solvents, and it is easy to lose its catalytic activity; it is difficult to recover biological enzymes, which is unreasonable economically, and it also causes difficulties in product separation and purification, which seriously affects th

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] After mixing 90 milliliters of n-heptane with 30 milliliters of tetrachlorethylene, inject it into a four-necked flask equipped with a thermometer, condenser, gas guide tube and stirring blade, add 0.25 gram of Span-60 surfactant and 0.85 gram of Tween-20 surfactant Heat up to 55°C. Under nitrogen protection and vigorous stirring, 17.5 ml of nanoscale Fe dissolved in 0.4 g of hydrophilic 3 o 4Magnetic particles, 3.2 g N,N'-methylenebisacrylamide, 0.54 g methacrylamide, 0.1 mL allyl glycidyl ether, 2.6 mL glycidyl methacrylate, and 0.55 g azobisisobutyronitrile Initiator solution in formamide. After the polymerization reaction at 55° C. for 4 hours, the stirring was stopped, and after the reaction was completed, the magnetic polymer microspheres were collected from the reaction system by applying an external magnetic field. Then the magnetic polymer microspheres can be used after washing with ethanol, soaking in n-heptane and vacuum drying, and the magnetic polymer mi...

Embodiment 2

[0031] After mixing 90 milliliters of n-heptane with 30 milliliters of tetrachlorethylene, inject it into a four-neck flask equipped with a thermometer, condenser, gas guide tube and stirring blade, add 0.34 gram of Span-60 surfactant and 0.7 gram of Tween-20 surfactant Heat up to 55°C. Under nitrogen protection and vigorous stirring, 17.5 ml of nano-scale Fe dissolved in 0.3 g of hydrophilic 3 o 4 Magnetic particles, 3.2 g N,N'-methylenebisacrylamide, 0.54 g methacrylamide, 1.35 mL allyl glycidyl ether, 1.35 mL glycidyl methacrylate, and 0.55 g azobisisobutyronitrile Initiator solution in formamide. After the polymerization reaction at 55° C. for 4 hours, the stirring was stopped, and after the reaction was completed, the magnetic polymer microspheres were collected from the reaction system by applying an external magnetic field. Then the magnetic polymer microspheres can be used after washing with ethanol, soaking in n-heptane and vacuum drying, and the magnetic polymer m...

Embodiment 3

[0033] After mixing 90 milliliters of n-heptane with 30 milliliters of tetrachlorethylene, inject it into a four-neck flask equipped with a thermometer, condenser, gas guide tube and stirring blade, add 0.34 gram of Span-60 surfactant and 0.7 gram of Tween-20 surfactant Heat up to 55°C. Under nitrogen protection and vigorous stirring, 17.5 ml of nano-scale Fe dissolved in 0.3 g of hydrophilic 3 o 4 Magnetic particles, 3.2 g N,N'-methylenebisacrylamide, 0.54 g methacrylamide, 1.8 mL allyl glycidyl ether, 0.9 mL glycidyl methacrylate, and 0.55 g azobisisobutyronitrile Initiator solution in formamide. After the polymerization reaction at 55° C. for 4 hours, the stirring was stopped, and after the reaction was completed, the magnetic polymer microspheres were collected from the reaction system by applying an external magnetic field. Then the magnetic polymer microspheres can be used after being washed with ethanol, soaked in n-heptane and vacuum dried, and the magnetic polymer ...

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Abstract

The invention discloses a magnetic polymer microsphere for enzyme immobilization and a relative preparation method, which uses hydrophilic nanometer magnetic particles as magnetic material, uses vinyl compound as functional monomer, uses composite surface activator as disperser and uses inverse suspension polymerization technique to prepare the macromolecule polymer pear carrier with narrow grain distribution and superparamagnetic and hydrophilic expoy group. The immobilization penicillin acylase prepared by the magnetic carrier has high apparent activity as 330IU/g (humidity), and the immobilization enzyme can be recovered easily to be circulated by external magnetic field, thereby improving the utilization of immobilization. The preparation method has simple process, easy operation, low production cost and support for large-scale production.

Description

technical field [0001] The invention relates to magnetic polymer microspheres used for immobilization of biological enzymes and a preparation method thereof. Specifically, a magnetic polymer microsphere with a narrow particle size distribution, a core-shell structure, superparamagnetism, hydrophilicity and High molecular polymer bead carrier containing epoxy groups. Background technique [0002] There are many shortcomings in the direct use of biological enzymes as catalysts: it is unstable in high temperature, strong acid, strong alkali and organic solvents, and it is easy to lose its catalytic activity; it is difficult to recover biological enzymes, which is unreasonable economically, and it also causes difficulties in product separation and purification, which seriously affects the product. Performance, the production process is difficult to achieve continuous operation, only one-time intermittent operation. The immobilized enzyme overcomes the above shortcomings of biol...

Claims

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Application Information

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IPC IPC(8): C08F220/56C08F222/38C08F220/32C08F216/14C08F2/44C08F2/32C08K3/22C12N11/08H01F1/42
Inventor 郭杨龙卢冠忠朱秀艳王筠松王莹詹望成王艳芹郭耘刘晓晖张志刚
Owner EAST CHINA UNIV OF SCI & TECH
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