Recovering method of effective components in amoxicillin enzymatic synthesis mother liquor by utilizing nanofiltration

Abstract

The invention discloses a recovering method of effective components in amoxicillin enzymatic synthesis mother liquor by utilizing nanofiltration. The method comprises the following steps: (1) regulating pH of the mother liquor; (2) hydrolyzing amoxicillin in the mother liquor by utilizing penicillin acylase, thus only D-hydroxyphenylglycine and 6-amino-penicillanic acid (6-amino-penicillanic acid) exist in the other liquor; (3) preparing concentrated mother liquor by nanofiltration; and (4) separating and recovering the D-hydroxyphenylglycine and the 6-amino-penicillanic acid (6-amino-penicillanic acid) by isoelectric point crystallization. The method has the advantages that: the recycling rate of an inactive lateral chain can be improved regarding certain amoxicillin synthetase with highhydrolysis activity, so that the enzymatic synthesis of amoxicillin is more economical.

Description

technical field [0001] The invention relates to a method for recovering active ingredients in the mother liquor of amoxicillin synthesized by enzymatic method, in particular to a method for concentrating by nanofiltration for the inactive side chain D-hydroxyphenylglycine and mother nucleus in the mother liquor of amoxicillin synthesized by enzymatic method A recovery method for 6-aminopenicillanic acid. Background technique [0002] Generally, there are two methods for synthesizing amoxicillin: chemical synthesis and enzymatic synthesis. The chemical synthesis of amoxicillin involves more chemical reaction steps and harsh reaction conditions, such as a low temperature environment of -50 degrees. During the reaction, organic solvents and chemical reagents, such as dichloromethane, N, N-dimethyl Acetamide, pyridine, triethylamine, acetone, pivaloyl chloride, etc., are not friendly to the environment. [0003] The enzymatic synthesis of amoxicillin can be completed by stirri...

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Problems solved by technology

[0008] To recover D-hydroxyphenylglycine and 6-aminopenicillanic acid in the mother liquor of amoxicillin, it is necessary to realize the separation of amoxicillin, D-hydroxyphenylglycine and 6-aminopenicillanic acid in the mother liquor. However, in the chemical structure The above three substances all contain amino and carboxyl groups, which are amphoteric compounds. The isoelectric points of the three substances are relatively close, so the separation can not be achieved by purely relying on isoelectric crystallization.

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