Aldehyde group mesoporous molecular sieve used for immobilization of biological enzyme and preparation method thereof

A mesoporous molecular sieve and aldehyde-based mesoporous technology, which is applied in the direction of being fixed on or in an inorganic carrier, can solve the problems of loss of catalytic activity of free enzymes, and achieve the effects of improving operational stability, activity and performance.

Inactive Publication Date: 2010-10-06
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method uses a large amount of organic solvents in the process of preparing immobilized enzymes, which easily causes the free enzymes to lose their catalytic activity.

Method used

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  • Aldehyde group mesoporous molecular sieve used for immobilization of biological enzyme and preparation method thereof
  • Aldehyde group mesoporous molecular sieve used for immobilization of biological enzyme and preparation method thereof
  • Aldehyde group mesoporous molecular sieve used for immobilization of biological enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] At room temperature, 2.0g Pluronic P123 (EO 20 PO 70 EO 20 ,M av =5800) was dissolved in 75mL of 1.6mol / L HCl solution; after P123 was completely dissolved, 0.023g of ammonium fluoride and 3.0g of 1,3,5-trimethylbenzene were added, then the solution was heated to 35°C and continued to stir for 45min , add 4.4g tetraethyl orthosilicate, and continue to stir at 35°C for 20 hours; transfer the solution into a hydrothermal crystallization kettle with a polytetrafluoroethylene liner, and conduct hydrothermal crystallization at 100°C for 24 hours; After the solution was cooled to room temperature, it was filtered to obtain a white solid, dried overnight in an oven at 100°C, and then calcined in a muffle furnace at a temperature programmed to 550°C for 8 hours to obtain a mesoporous molecular sieve.

[0036] use 60 Schematic diagram of the functionalization of aldehyde groups on the surface of mesoporous molecular sieves and the immobilization of enzymes by Co-γ-ray pre-ir...

Embodiment 2

[0038] The preparation method of the mesoporous molecular sieve is the same as that of Example 1. Put 1.0g of the above-mentioned mesoporous molecular sieve in 60 Co-γ-ray pre-irradiation, the irradiation dose is 24kGy, the sample is transferred to 50mL 90% acrolein aqueous solution after pre-irradiation, placed in an ice-water bath, vacuumed, filled with nitrogen, and then placed in a water bath at 30 °C React for 30 hours; filter the mesoporous molecular sieve, extract with ethanol in a Soxhlet extractor to remove acrolein polymer, and then dry in a vacuum oven at 75°C for 18 hours to obtain an aldehyde-based mesoporous molecular sieve. The above-mentioned aldehyde-based mesoporous molecular sieve was used to immobilize penicillin acylase, and the activity of the obtained immobilized enzyme was 8760 U / g. After 10 cycles of use, the immobilized enzyme retained 81% of the initial activity.

Embodiment 3

[0040] The preparation method of the mesoporous molecular sieve is the same as that of Example 1. Put 1.0g of the above-mentioned mesoporous molecular sieve in 60 Co-γ-ray pre-irradiation, the irradiation dose is 120kGy, the sample is transferred to 200mL 10% acrolein aqueous solution after pre-irradiation, placed in an ice-water bath, vacuumed, filled with nitrogen, and then placed in a water bath at 80 °C React for 10 hours; filter the mesoporous molecular sieve, extract with ethanol in a Soxhlet extractor to remove the acrolein polymer, and then dry in a vacuum oven at 100° C. for 12 hours to obtain the aldehyde-based mesoporous molecular sieve. The above-mentioned aldehyde-based mesoporous molecular sieve was used to immobilize penicillin acylase, and the activity of the obtained immobilized enzyme was 8920 U / g. After 10 cycles of use, the immobilized enzyme retained 80% of the initial activity.

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Abstract

The invention discloses an aldehyde group mesoporous molecular sieve used for immobilization of a biological enzyme and a preparation method thereof. In the preparation method, a 60 Co-gamma ray pre-irradiation grafting acrolein technique or gamma-aldehyde propyl trimethoxy silane is used for performing functionalized modification on the surface of the mesoporous molecular sieve, an aldehyde propyl functional group with a shorter chain length is introduced to the surface of the mesoporous molecular sieve, the influences, caused by the surface functionalizing process, on the aperture, the specific area and the pore volume of the mesoporous molecular sieve are reduced as much as possible, and further activation is not needed to ensure that the biological enzyme is directly immobilized on the surface of the mesoporous molecular sieve in a covalent bonding mode, and the performance of the immobilized enzyme is improved. The aldehyde group mesoporous molecular sieve can be applied to the immobilization of water-soluble enzymes such as penicillin acylase, glucose isomerase, transglucosidase, trypsase, amylase and the like, and is particularly suitable for the immobilization of the penicillin acylase; and the obtained immobilized enzyme has the activity of 8895 U / g, and after 10 times of recycling, the immobilized enzyme maintains 92 percent of the initial activity.

Description

technical field [0001] The invention relates to an aldehyde-based mesoporous molecular sieve for immobilization of biological enzymes and a preparation method thereof, specifically, using 60 Co-γ-ray pre-irradiation grafting acrolein technology or γ-aldehyde propyl trimethoxysilane functional modification on the surface of mesoporous molecular sieves, introducing short-chain aldehyde propyl functional groups (-CH 2 -CH 2 -CHO), and then immobilize biological enzymes on the surface of mesoporous molecular sieves in a covalent manner to improve the performance of the immobilized enzymes. Background technique [0002] There are many deficiencies in using free enzyme directly in the catalytic process, such as instability in high temperature, strong acid, strong alkali and organic solvent, and easy loss of catalytic activity; free enzyme recovery is difficult, economically unreasonable, and the product is difficult to separate and purify, seriously Affect product quality; the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14
Inventor 郭杨龙高振源田程程卢冠忠詹望成郭耘王筠松王艳芹刘晓晖张志刚
Owner EAST CHINA UNIV OF SCI & TECH
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