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Strain capable of simultaneously degrading two isomers of chiral herbicide quizalofop-p-ethyl and application of strain

A technology of herbicide and degrading bacteria, which is applied in the field of biotechnology, to achieve low production and use costs, wide application potential and value, and the effect of protecting the ecological environment

Active Publication Date: 2021-12-17
HUAIBEI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the (S)-type enantiomer has no herbicidal activity, there is a phenomenon of mutual conversion between the two isomers in the environment

Method used

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  • Strain capable of simultaneously degrading two isomers of chiral herbicide quizalofop-p-ethyl and application of strain
  • Strain capable of simultaneously degrading two isomers of chiral herbicide quizalofop-p-ethyl and application of strain
  • Strain capable of simultaneously degrading two isomers of chiral herbicide quizalofop-p-ethyl and application of strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1, isolation and identification of bacterial strain

[0040] The invention provides a bacterial strain capable of efficiently degrading two chiral isomers of a chiral herbicide (R,S)-QE and the bacterial agent produced therefrom. The bacterial strain used is a Gram-negative bacterial strain S1, which is isolated from Nanjing, Jiangsu In the soil of an abandoned pesticide factory. The specific isolation and screening methods for strains are as follows:

[0041] Take 10.0 g of soil sample and add it to 100 mL of liquid inorganic salt medium (hereinafter referred to as MSM) containing 30 mg / L (R,S)-QE, and cultivate it on a shaker at 30°C and 180 rpm for 7 days, and inoculate at 15% (v / v) Transplanted into fresh same medium, enriched and subcultured four times. Use an ultraviolet spectrophotometer to scan in the range of 200-350nm to detect the degradation effect of the fifth-generation enrichment solution. Dilute the effective enrichment solution on LB solid...

Embodiment 2

[0043] Embodiment 2, laboratory degradation experiment

[0044] 2.1 Preparation of seed solution

[0045] Introduce strain S1 into 100 mL LB medium, culture on a shaker at 30°C and 180 rpm, collect the bacteria by centrifugation at 6,000 rpm after 48 h, wash the bacteria twice with sterilized MSM, and finally wash them with 10 mL of sterilized MSM Resuspended and used as seed solution for later use.

[0046] 2.2 Degradation of chiral herbicide (R,S)-QE by strain S1

[0047] Inoculate the strain S1 into 100 mL MSM containing 30 mg / L (R,S)-QE at a 5% inoculum amount, cultivate on a shaker at 30°C and 180 rpm, and sample 3 mL every 12 hours until the fourth day . The residual amount of (R,S)-QE was detected, the degradation rate was calculated, and the time-degradation curve of the chiral herbicide (R,S)-QE of strain S1 was drawn. Such as image 3 , strain S1 could completely degrade 30mg / L (R,S)-QE within 96 h. The results of liquid chromatography showed that strain S1 cou...

Embodiment 3

[0058] Embodiment 3, soil degradation experiment

[0059] The vegetable garden soil was taken as the test soil sample. Pass the soil sample through a 2mm sieve, take a certain amount of (R,S)-QE, fenoxaprop-p-p-p, and fluazifop-p-p-pf powder respectively and dissolve them in 10mL of acetone, then soak diatomaceous earth to make the pesticides completely adsorption. The soaked diatomaceous earth is placed in a fume hood to dry, and it is mixed into the soil so that the concentration of the pesticide in the soil is about 30mg / kg. Take 500g of each soil sample and culture it in a constant temperature incubator at 30°C. Inoculate the S1 seed solution with 10% inoculum amount, and use no inoculation as a control. The water holding capacity of the soil is maintained at 60%. After culturing for 7 days, 3 samples were taken for each treatment, and each sample weighed 20 g. Samples were extracted with twice the volume of dichloromethane and placed in a mechanical shaker for 2 hours ...

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Abstract

The invention discloses a strain capable of simultaneously degrading two isomers of chiral herbicide quizalofop-p-ethyl and application of the strain. The strain S1 is identified as Brevundimonas sp. and deposited in the China Center for Type Culture Collection on May 24, 2021, and the strain preservation number is CCTCC M 2021604. The degrading strain S1 disclosed by the invention can degrade the two isomers of the aryloxy phenoxypropionate herbicide quizalofop-p-ethyl, quizalofop-p-ethyl, fluazifop-p-butyl, fluazifop-p-butyl or fenoxaprop-p-ethyl and the like in a broad-spectrum manner, a degrading bacterial agent product can reduce the residual quantity of (R, S)-QE and the like in soil by 88.6% or above, the problems of pollution of the aryloxy phenoxypropionate herbicide in soil and water environments, phytotoxicity to crops and the like in the agricultural production process can be effectively solved, and the ecological environment is protected.

Description

technical field [0001] The invention relates to a degrading bacterial strain capable of simultaneously degrading two isomers of the chiral herbicide quizalofop and the bacterial agent produced therefrom, which belongs to the high-tech field of biology and uses microorganisms to efficiently degrade herbicide residues, and is suitable for soil, Microbiologically enhanced restoration of water bodies and sludge. Background technique [0002] The widespread use of chemical pesticides (including herbicides, fungicides and insecticides) has played an irreplaceable role in increasing agricultural production and ensuring income, and the use of chemical herbicides has ranked first. According to statistics, about 40% of the commonly used pesticides in my country have a chiral structure (the molecular structure of the compound and its mirror image cannot overlap each other), and herbicides with a chiral structure (chiral herbicides) account for a large proportion. Studies have shown th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02A62D101/04A62D101/28C12R1/01
CPCC12N1/20A62D3/02A62D2101/04A62D2101/28
Inventor 张龙宋嫚李峰毛振波王光利刘远
Owner HUAIBEI NORMAL UNIVERSITY
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