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Strain Am101 capable of degrading various beta-lactam antibiotics and application of strain Am101
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A technology of lactams and antibiotics, applied in the field of microorganisms, achieves the effects of wide degradation spectrum, convenient use and simple process
Active Publication Date: 2020-02-14
BEIJING INSTITUTE OF TECHNOLOGYGY
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However, there are few reported strains that can rapidly and efficiently degrade β-lactam antibiotics, especially those that degrade multiple β-lactam antibiotics at the same time
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Embodiment 1
[0020] 1. Screening of β-lactam antibiotic-degrading strain Am101
[0025] Medium No. Ⅱ: beef extract 1.5g, peptone 6.0g, yeast extract powder 6.0g, glucose 1.0g, water 1L, sterilized at 115°C for 20min. Add 10g-15g of agar to the solid medium.
[0026] Inorganic salt medium: (NH 4 ) 2 NO 4 2.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, CaCl 2 0.1g, MnSO 4 h 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, sterilized at 121°C for 15-20min. Add 10g-15g of agar to the solid medium.
[0027] Martin's ...
Embodiment 2
[0046] Growth and degradation characteristics of strain Am101
[0047] 1. Growth characteristics of strain Am101
[0048] The strain Am101 was inoculated in different solid media such as beef extract peptone medium, Martin's medium, potato medium, and inorganic salt medium. Potassium, amoxicillin, cefuroxime sodium, and ceftiofur sodium can also grow in the beef extract peptone medium, and the cultivation is easy.
[0049] The suitable culture conditions of the strain Am101 were studied by single factor test, the settings included temperature 35°C-55°C, pH value 5-9, concentration of β-lactam antibiotics 50mg / L-150mg / L, culture time 3-7 Today, it was found that the strain Am101 can grow at 55°C, and can grow at a pH value of 5-9 and a concentration of β-lactam antibiotics of 50mg / L-150mg / L.
[0050] Inoculate the strain Am101 in the beef extract peptone liquid medium, place it at 28--30°C, shake it at 150-180r / min, take samples regularly, measure the OD value at 600nm, and o...
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technical field [0001] The invention relates to a degrading strain Bacillus tequilensis Am101 capable of degrading multiple β-lactam antibiotics, belonging to the technical field of microorganisms. Background technique [0002] β-lactam antibiotics refer to a large class of antibiotics containing a β-lactam ring in their molecular structure, including penicillins with 6-APA as their nucleus and cephalosporins with 7-ACA as their nucleus. It is widely used in animal husbandry, fishery and clinical because of its broad antibacterial spectrum, strong antibacterial effect and low price. It is the most widely used class of existing antibiotics. [0003] During the fermentation and production of β-lactam antibiotics, a large amount of bacterial residue and waste water will be produced. If they are not properly treated and discharged into the environment, they may remain in soil, water and food; during the use of antibiotics, only a small amount Antibiotics are metabolized by huma...
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