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CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and application thereof

A technology of cephalosporins and antibiotics, applied in the field of microorganisms, can solve problems such as scarcity of degrading strains

Active Publication Date: 2018-04-24
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few research reports on the biodegradation of cephalosporin antibiotics at present, and the degrading strains screened are even scarcer, mainly bacteria, which can only degrade one of the cephalosporin antibiotics.

Method used

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  • CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and application thereof
  • CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and application thereof
  • CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Screening of cephalosporin antibiotic degrading strain CM1

[0025] 1. Material preparation

[0026] The source of bacteria samples: fresh bacteria residues from an antibiotic manufacturer.

[0027] Potato dextrose medium (PDA): 200g potatoes, 20g glucose, 1L distilled water, natural pH, sterilized at 115°C for 30min. Add 10-15g of agar to the solid medium.

[0028] Martin's Medium: KH 2 PO 4 1.0g, MgSO 4 7H 2 O 0.5g, glucose 10.0g, peptone 5.0g, distilled water 1L, pH natural, sterilized at 115°C for 15min. Add 10g-15g of agar to the solid medium.

[0029] Inorganic salt medium: (NH 4 ) 2 NO 4 1.0g, K 2 HPO 4 0.5g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.5g, NaCl 0.2g, CaCl 2 0.1g, MnSO 4 h 2 O 0.01g, FeSO 4 ·7H 2 O 0.01g, distilled water 1L, pH7.0, sterilize at 121℃ for 15-20min. Add 10g-15g of agar to the solid medium.

[0030] Medium No. Ⅱ: beef extract 1.5g, peptone 6.0g, yeast extract powder 6.0g, glucose 1.0g, water 1L, sterilized at 115°C...

Embodiment 2

[0064] Growth and degradation characteristics of strain CM1

[0065] 1. Growth of strain CM1

[0066] 1. Growth of strain CM1 on different media

[0067] It has been found through experiments that the strain CM1 can grow in various media, such as Martin's medium, potato medium, Chase medium, and inorganic salt medium. Fast growing and easy to cultivate. Streak the strain CM1 on the potato solid medium, and culture it at room temperature (20°C-37°C) for 5d-10d, the strain CM1 can cover a 9cm plate, and its growth curve is shown in Figure 4 .

[0068] 2. Growth experiment of strain CM1 on medium with different concentrations of cephalosporin antibiotics

[0069] Strain CM1 can grow well in the medium with cephalosporin antibiotics as the only carbon source. Prepare cephradine, cephalexin, and cefuroxime sodium as the sole carbon source and the concentrations of the three cephalosporin antibiotics are 50mg / L, 100mg / L, 500mg / L, 1000mg / L, 5000mg / L, and 10000mg / L respectively....

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Abstract

The invention relates to CM1 (Galactomyces candidum) capable of degrading cephalosporin antibiotics and belongs to the technical field of microorganisms. The bacterial strain is preserved in the common microorganism center of the Chinese microorganism bacterial strain preservation management committee on November 10, 2017, and the preservation number is CGMCC NO.14633. The bacterial strain is easyto culture, grows quickly, and has the function of degrading cephalosporin antibiotics, and meanwhile the toxicity and inhibition effects of cephalosporin antibiotics on gram-positive bacteria can bedegraded and removed. The CM1 can be applied to fungicide production and treatment of waste of cephalosporin antibiotics production enterprises and residual cephalosporin antibiotics in the environment, and the harm of cephalosporin antibiotics to the environment and people is reduced.

Description

technical field [0001] The invention relates to a strain of Geotrichum candidum (Galactomyces candidum) CM1 capable of degrading cephalosporin antibiotics, belonging to the technical field of microorganisms. Background technique [0002] Cephalosporin antibiotics are an important class of β-lactam antibiotics discovered after penicillin. They have the advantages of strong antibacterial activity, broad antibacterial spectrum and resistance to penicillinase, and are widely used in the prevention and treatment of human diseases. According to reports, after antibiotics enter the human body and animals, they cannot be completely metabolized and absorbed. 75%-90% of antibiotics will enter the environment with excreta in the form of drug prototypes or metabolites, and the toxicity of some antibiotic metabolites is even higher than matrix. On the other hand, during the fermentation process of cephalosporin antibiotics, a large amount of bacteria residue and waste water will be prod...

Claims

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Application Information

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IPC IPC(8): C12N1/14A62D3/02C12R1/645A62D101/28
CPCA62D3/02A62D2101/28C12N1/145C12R2001/645
Inventor 李艳菊宫宇杨正礼张爱平王梓诺
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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