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Diaphorobacter strain and application thereof

A technology of bacteria strains and bacteria agents, applied in the field of Diaphorobacter strains, can solve the problems of difficult wastewater discharge and other problems, and achieve the effects of reducing production and use costs, protecting the ecological environment, and being easy to use

Inactive Publication Date: 2010-11-24
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional wastewater treatment is difficult to discharge wastewater containing the above components up to standard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, the acquisition and identification of bacterial strain SG-51

[0026] 1. Obtaining of strain SG-51

[0027] In April 2008, 2g of coking wastewater activated sludge was taken from Shaoguan, Guangdong, and added to a basal salt medium containing pyridine and phenol to cultivate for 7 days. Days later, single colonies were picked and streaked for purification and preserved.

[0028] 2. Identification of strain SG-51

[0029] Morphological characteristics: The bacteria are short rod-shaped.

[0030] Physiological and biochemical characteristics: Gram-stain-positive bacteria (Gram-stain positive); catalase, nitrate reduction reaction is positive; gelatin hydrolysis, nitrite reduction reaction, methyl red reaction, glucose fermentation acid production, oxidase , V.P. reaction, and indole reaction are negative; starch cannot be hydrolyzed, and citric acid and tartaric acid cannot be used;

[0031] Molecular identification results: See sequence 1 in the sequen...

Embodiment 2

[0034] Embodiment 2, the degradation of bacterial strain SG-51 to different compounds in basal salt liquid medium

[0035] Basal salt liquid medium: mix 1.0g NaCl, 1.0g NH 4 NO 3 , 1.5g K 2 HPO 4 , 0.5g KH 2 PO 4 and 0.2g MgSO 4 ·7H 2 O was made up to 1L with water.

[0036] Pick a single colony of SG-51 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial solution.

[0037]Into 100ml of solution A (or solution B or solution C or solution D or solution E or solution F or solution G or solution H), insert 2ml of fresh bacterial solution, and culture it on a shaker at 30°C (170 rpm), after 3 days (72 hours) Samples were taken to determine the degradation rate of the compound. Degradation rate=(1-the amount of the compound at the end of the reaction / the amount of the compound at the beginning of the reaction)×100%.

[0038] The preparation method of solution A: add phenol into the basic salt liquid medium, and the final...

Embodiment 3

[0049] Example 3. Degradation of different compounds by bacterial strain SG-51 in coking wastewater

[0050] Pick a single colony of SG-51 and put it in 3ml LB liquid medium, shake it at 30°C and 165 rpm for 24 hours to obtain fresh bacterial solution.

[0051] Measure 100ml of coking wastewater, and detect the concentration (initial concentration) of phenol and cresol therein. The concentration of phenol is 400mg / L; the concentration of cresol is 100mg / L.

[0052] Inject 2ml of fresh bacterial solution, mix well, place in a shaker at 30°C and 165 rpm for culture, and take a sample after 4 days (96 hours) to determine the degradation rate of the compound. The results are shown in Table 2.

[0053] Table 2 Degradation characteristics of strain SG-51 to different compounds in coking wastewater (4 days)

[0054]

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PUM

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Abstract

The invention discloses a Diaphorobacter strain and application thereof. The strain is Diaphorobacter sp. SG-51 CGMCC No.3511. A bacterial agent provided by the invention has the active ingredient of the strain Diaphorobacter sp. SG-51 CGMCC No.3511. The strain has wide degradation spectrum and has the degradation rate for various toxic compounds of over 90 percent. The bacterial agent is a novel aromatic organic pollutant degradation bacterial agent, and has the advantages of low production and use cost, convenient use, wide degradation spectrum and good removing effect. The strain and the bacterial agent are suitable for treating wastewater generated in the modern coking and vaporization processes, and organic wastewater containing phenol, methylphenol, parachlorophenol and pyridine generated in other production processes of the petrochemical industry, and can ensure that corresponding organic matter residue meets the wastewater emission standard. The invention successfully solves the problem of low removing effect on phenol and derivatives thereof, and benzene or other heterocyclic compounds from the wastewater treatment, reduces workload in the production process and using process, reduces production and use cost, and has significance for protecting ecological environment and protecting human health.

Description

technical field [0001] The invention relates to a Diaphorobacter bacterial strain and application thereof. Background technique [0002] Pyridine, phenol, cresol and their derivatives are important chemical raw materials, and are also the main components of coking wastewater and vaporization wastewater generated during coal coking and vaporization. Studies have found that these compounds are harmful to humans and environmental organisms. Traditional wastewater treatment is difficult to discharge wastewater containing the above components up to standard. Microorganisms are often used as an important tool for environmental pollution remediation because of their powerful degradation functions and strong adaptability. Contents of the invention [0003] The purpose of the present invention is to provide a Diaphorobacter bacterial strain and application thereof. [0004] The bacterial strain (Diaphorobacter sp.) provided by the present invention, isolated from the coking waste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/01C02F101/38C02F101/34C02F103/34A62D101/28A62D101/26
Inventor 吴晓磊孙纪全汤岳琴刘伟强赵晶晶郭鹏
Owner PEKING UNIV
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